Brat1 (NM_001276287) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA881470; Baa; Baat1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229071 representing NM_001276287 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCATTTTGCACTCTCGGGCCTAATTCCCGTAGCGGAGGGATGCGGGGGCGGGATTAGAACCCCTA GGCTAACTCGCAGTCGCCGAGAGCGCACCTCTTACCCGGATTTGGGAGGAACTGCCTGCCTGATCATGGA CCCAGAATGCTCCAGGCTCCTCCCGGCTCTCTGTGCTGTTTTGGCAGATCCCAGACAGCTGGTGGCAGAT GACACCTGCTTGGAGAAACTGCTGGACTGGTTTAAAACAGTGACAGAGGCAGAGTCTAGCCTCCAACTAC TACAGGACCATCCCTGCTTAATGGAGCTCCTGTCCCATGTGCTGAAGCCACAGGACGTGAGCCCTAGGGT CCTCTCCTTTGCTCTGCGCCTTGTTGGGGTCTTCGCAGCCCAGGAAGACTGTTTTGAGTACCTTCAGCAG GGAGAGTTGTTGCTGGGGCTCTTTGGGGAGTCAGGTGCCCCCGGCTGGGCAGCCTGGAGCATCCCAAGTG TGCGCAGCGGCTGGATCCAGGGTCTGTGCTACCTGGCACACCACCCTAGCGCCCTGCACTTCCTGGCTGA CAGTGGTGCTGTGGACACGCTCTTCTCCTTGCAGGGAGACCCCAGCCTGTTCGTCGCCTCAGCAGCCAGC CAGCTCCTAGTACATATCCTGGCTCTGTCCATGCAAGGTGGAGCCCCAGGGTCCCCCGTCCCTGAAGCTG CTGCTTGGCCTATGTGTGCCCAGAAGATTGTGAACCATGTGGATGAGTCCCTGCATGCCAAAGCCACCCC CCAGGTCACACAGGCCTTGAATGTCCTGACTACGACCTTCGGGCGCTGCCATAACCCCTGGACAGGGGTC CTCTGGGAGCGGCTAAGTCCCCCTGTTGCCCGCCTGTTTGAGAGAGACCCCATTCCAGCCGTGCACGCGC TCATGGACCTTCTTCTTAGTGTGGCCAGGTCGCCTGTGTTGAATTTTGCAGCCTGTGGCCTGTGGGAGAT GCTGGCCCAGACTCTGAGCCGCCTGAGCCCCATACAAGCTGGGCCTCTAGCCCTGGGGACCCTGAAACTT CAGCACTGTCCCCAGGAATTGAGGACCCAGGCCTTTGGAGTCCTCCTACAGCCACTGGCCTGTATCCTGA AAGCTACCACTCAGGCCCCTGGACCTCCAGGCTTGCTGGATGGGACTGTGGGTAGCTTGCTGACTGTGGA TATACTCTTGGCTTCAAAGTCAGCCTGTGTGGGACTCCTTTGCCAGACTCTGGCTCACCTGGAGGAGCTG CAGATGCTGCCCCAGTGCCCCTCACCCTGGCCACAGGTGCATCTGCTGCAAGCTGCTTTGACTATATTGC ATCTCTGTGATGGCTCAGCGGACCCCAGCTCCAGTGCAGGAGGCCGTCTCTGTGGGACTCTGGGTGGCTG TGTTCGTGTCCAGCGAGCAGCCCTTGACTTCTTGGGGACCCTGTCTCAGGGGACAAGCCCCCTGGAGTTG GTTCTGGAGGTATTTGCTGTTCTCCTGAAGACCCTGGAGAGCCCAGAGTCCAGCCCCATGGTCCTAAAGA AGGCCTTCCAGGCCACACTCAGATGGCTCCAGAACCCACACAAGACCCCCAGCAGCTCTGATCTCAGCTC CGACGCCCTGCTGTTTCTCGGAGAGCTGTTCCCCATACTACAGAAGCGCCTATGCAGCCCATGTTGGGAG GTGAGGGACTCTGCCCTGGAGTTCCTGACGCATCTGATCCGACACTGGGGAGGGCAGGCTGACTTCAGAG AGGCACTGCGTTCCTCAGAAGTACCCACACTTGCCCTCCAGCTTCTCCAAGACCCAGAGAGTTACGTCCG AGCAAGTGCCGTGGGTGCCGCTGGGCAGCTCTCCAGCCAGGGTCTGCAGGCCGCTCCCGCTAGCCCCGAG AACTCGCAGGCCCAACAGGGCCTACTCATGGACCTTATGCATATCCTGTCCACTGACTCAGAGGGCTTCC CTCGAAGGGCTGTGTTACGGGTCTTCACGGACTGGCTGAGGGATGGCCATGCTGACGTGGTTCGAGACAC GGAGTGGTTCGTGGCCACTGTTCTCCAGGCAGTGAGCCGGGATCTGGACTGGGAGGTCCGAGTGCAGGGT TTGGAGCTGGCACGGGTGTTCCTCACCCAGGCACTGGGCCAGCCCAGCCTCCACTGTCCCTATACAGTGG GCCTGCCCAGGGCCTCCTCTCCCCGCCCACACCCGGAATTCTTGCAGACTCTCTGCCGTCTGCCGCTCTT TGAGTTTGCCTTTTGTGCCTTGCTTGACTGTGACCGACCGGTGGCCCAAAAGGCCTGTGACCTGCTCCTC TTCTTGAGGGACAAGACAGTTCCCTGCAGTAGCCCTCGGGAGGCAGGGGACAGCCCCAACTCAGCCTCAG TGGAGGCTGCCCTGCAGAGGTGGCGGGAAGGAGAGCAGGCCCAGCCCCTAGGGGACCTGGATCCTGAGGC CATGCTAGCCATCCTCAGGGCCTTAGACCTGGAGGGCCTGCAGGGCAGGCTGGCCAAGAGCAGCGACCAT GTGGAGAAGAGCCCACAGTCCCTGCTGCAGGACATGCTGGCCACGGTGGGTGTGTTGGAGGAGAACGAAG CTGACTGCTACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276287
Insert Size	2604 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276287.1, NP_001263216.1
RefSeq Size	3451 bp
RefSeq ORF	2604 bp
Locus ID	231841
UniProt ID	Q8C3R1
Gene Summary	A similar gene in human encodes a Breast Cancer 1 (BRCA1) interacting protein that is involved in cell cycle checkpoint signaling. The similar human protein is localized to DNA double strand breaks caused by ionizing radiation, and regulates cellular DNA damage response through interactions with Ataxia Telangiectasia Mutated (ATM) and DNA-dependent Protein Kinase. A pseudogene of this gene is located on chromosome 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (3) represents the longest transcript and encodes the longer isoform (3).

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