Dsc1 (NM_001291804) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110020A10Rik; AI507491; Dsc
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229105 representing NM_001291804 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGGCCTGTGCTGCCCCAGGGAGCACCTTCTCTAAGCAGCTCCTTTTCTTTCTCCTGGTTCTGG TATTATTTTGTGATGCCTGTCAAAAAGTTTCCCTTCACGTTCCCTCTCATCTTAAGGCTGAAACACCTGT AGGCAAAGTGAATCTGGAAGAGTGTCTCAAATCACCCAGCCTGATCCTGTCCAGTGACCCAGCCTTCAGA ATTCTAGAAGACGGCACAATTTACACAACACATGACCTGCTTTTGTCTTCTGAAAAGAGGGGGTTCTCCA TCTTGCTCTCAGATGGTCAAGGGCAGGAACAAAAGAAGCTAGAAGTTGTACTGTCAGCAAGAGAAAAAAA GGTTTTTAGGAAAAGACATACCAAAGAGCCAGTACACAACCGCAGTAAGAGAAGATGGGCTCCTATTCCA TGTTCACTGATGGAGAACTCTTTGGGTCCATTTCCACAACACATTCAGCAGATCCAATCTGATGCTGCCC AGAATTACACCATCTTTTACTCTATCAGTGGACCAGGAGTAGACAAAGAGCCTTACAATTTGTTCTACAT AGAGAAAGACACCGGGGACATCTATTGTACCCGAAGCATTGACCGTGAACAGTATGACCAATTTTTGGTA TATGGATATGCAACAACTGCTGATGGCTATGCCCCAGACTACCCTCTCCCCCTGCTGTTTAAGGTTGAAG ATGACAATGACAATGCCCCATATTTTGAAACCAAATTAACAGTTTTTAGTGTGCCTGAAAATTGCCGATC TGGAACTTCAGTGGGACAAGTGACTGCCATAGACAAGGATGAGCCAGGAACTCTACACACTCGTCTGAAG TACAAAATCTTACAACAAATCCCAGACCAGCCAAAGCACTTCTCCATCCACCCAGACACAGGTGTGATCA CTACCACCACACCTTTACTGGACAGAGAGAAATGTGACACTTACAAGCTAGTGATGGAGGTACGAGACAT GGGGGGCCAGCCCTTTGGTCTGTTCACCACAGGAACAATCACCATCTCACTGGAGGATGAGAATGACAAC TCGCCATACTTCACCCAAACTTCTTATACTACAGAAGTAGAAGAAAACAGAATTGATGTTGAGATTCTGA GAATGGTGGTGCACGACCAAGATTTGCCCAACACCCCCCACTCGAAGGCGGTATACACAATCCTAAAGGG GAACGAAAACGGGAACTTCAAAATCACAACGGATCCAAATACAAATGAAGGGGTCTTGTGTGTTGTCAAG CCACTGAACTATGAAGTCAGCCGCCAAGTCACTCTGCAAATTGGTGTCCTTAATGAAGCCCAGTTCACTA ACGCTGCCAACGCACAACCACCAACAATGTGCACCACAACTGTCACTGTGAAAATTAAAGACAGAGATGA GGGCCCTGAGTGCCAGCCACCAGTCAAAGTCATTCAGAGCAAAGATGGACTGCCAGCGGGCCAAGAGCTC CTGGGCTACAAAGCGGTGGACCCAGAGACAAGCAGTGGTGAAGGCTTAAGGTATGAGATGGTAGGAGATG AAGATAATTGGTTTGAGATTAACAAAATCACCGGAGACTTGAGAACTGTGAAAGTACTGGACAGAGAGTC GAAGTTTGTGAAAAACAACCAGTACAATATTTCAGTAGTGGCGACAGATACAGCTGGCCGATCTTGCACA GGAACACTGGTAGTTCTTCTGGAAGATTTTAATGACCACCCACCACAGATTGACAAGGAAGTGACCATTT GTCAGCAGGAGAAGGATTTTGCTGTTTTGGAACCTATAGATTTAGATGGGCCAGATAATGGTCCACCTTT TCAGTTCCTTCTGGATAATTCTTCCAGCAAACTTTGGACTCTAGAATCACAGGATGGTAAACGTGCCATT CTTCGTCAACGACACAATCTTAATTATAACTATTATTCTGTGCCAATCCAAATACAAGACAGGCATGGTT TTTCCGCAAAACATGTATTATCAGTGCGAGTGTGTGACTGTACAACTCCAACAGAATGTAGAATGGCTGT TAAGGAAGAGAGGGATGCTAAACCAAATATAATCCTTGGGAAATGGGCCATTCTTGCCATGGTCCTGGGC TCTGCATTGCTACTGTGCATTTTGTTTACATGCTTCTGTGTGACCACTACCAAGAGAACAGTCAAGAAAT GCTTCCCAGATGATGTAGCTCAGCAAAATTTAATTGTATCAAATACTGAAGGACCAGGAGAAGAAGTGAC GGAAGCCAATATTAGACTCCCCACACAGACAGCCAACATCTGTGATACAAGCATGTCTGTTGGGACTCTT GGTGGTCAAGGAATCAAAACACAGCAAAGTTTTGAGATGGTCAAAGGAGGCCACACCTTGGAGTCCCACA AGGGAGGAGTGCTAGGAGCAGCAGAGCCTGGCCGATATGCTTACACAGACTGGCAAACTTTCACTCAACC TCGGCTTGGTGAAAAGGTGTATTTGTGTGGGCAAGCTGAAGAACACAAGCATTGTGAAGACTACGTTCGC CCATATAACTATGAAGGCAAAGGCTCCATGGCTGGCTCTGTGGGCTGCTGCAGTGATCGGCAGGAGGAAG AAGGGCTAGAGTTTTTAGATCAATTGGAACCAAAATTTAGGACATTAGCAAAGACATGTGTGAAGAAATA A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291804
Insert Size	2661 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291804.1, NP_001278733.1
RefSeq Size	4961 bp
RefSeq ORF	2661 bp
Locus ID	13505
UniProt ID	P55849
Gene Summary	This gene encodes a member of the cadherin family of proteins that mediates adhesion in desmosomes. The encoded preproprotein undergoes proteolytic processing to generate the mature, functional protein. Mice lacking the encoded protein exhibit epidermal fragility together with defects of epidermal barrier and differentiation. The neonatal mice lacking the encoded protein exhibit epidermal lesions and older mice develop chronic dermatitis. This gene is located in a cluster of desmosomal cadherin genes on chromosome 18. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Jan 2016] Transcript Variant: This variant (1, also known as Dsc1a) represents the shorter transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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