Usp47 (NM_177249) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4930502N04Rik; A630020C16Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229572 representing NM_177249 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCCCGGCGAGGAGAACCAACTGGTCCCGAAGGAGGATGTGTTTTGGAGATGCAGACAAAATATCT TTGATGAAATGAAGAAGAAATTTTTACAGATAGAAAATGCTGCTGAAGAACCTAGAGTCTTATGTATAAT ACAAGACACTACTAACTCGAAGACAGTGAGTGAACGGATCACATTAAATTTACCAGCATCTACCCCAGTC CGAAAGCTCTTTGAAGATGTGGCCAACAAAGTAGGCTACATAAATGGAACCTTTGATTTGACCAGGGAAA ATGGAGTCACTACTGCTGACATGGCACCACTGGATCACACCAGTGACAAGTCTCTTCTTGATGCTAATTT TGAGCCAGGAAAGAAGAACTTTCTGCATTTGACAGATAAAGATGGTGAACCGCCTCAGATGTTGTTGGAG GACTCCAATAACGTGGATGACAGCGTGCATGACAGGTTCATAGGTCCACTGCCAAGAGAAGGTTCTGTGG CCTCTACCAATGATTATGTCAGCCAGAACTACTCCTATTCATCAATTTTGAACAAGTCAGAAACTGGTTA TGTGGGATTAGTAAACCAGGCGATGACGTGCTATTTGAATAGCCTTTTGCAAACACTTTTTATGACTCCT GAATTTAGGAATGCATTATATAAGTGGGAATTTGAAGACTCTGAAGAAGATCCTGTAACGAGTATCCCAT ACCAGCTTCAAAGGCTTTTTGTTTTGTTACAAACCAGCAAAAAGAGAGCAATTGAGACCACAGATGTTAC AAGGAGCTTTGGATGGGATAGCAGTGAGGCTTGGCAACAGCATGATGTACAAGAACTGTGCCGTGTTATG TTTGATGCTTTGGAACAGAAATGGAAGCAAACAGAACAGGCTGATCTTATAAATGAGCTGTACCAAGGCA AGCTGAAAGATTATGTGAGGTGTCTGGAATGCGGTTATGAAGGCTGGAGAATTGACACGTATCTTGATAT TCCACTGGTCATCCGACCTTATGGGTCCAGCCAAGCGTTTGCTAGTGTGGAAGAAGCGTTGCATGCATTT ATTCAACCAGAGATCTTGGATGGCCCAAATCAATATTTTTGTGAACGTTGTAAGAAGAAGTGTGATGCTC GGAAGGGCCTTCGGTTTTTGCATTTCCCCTATCTGCTGACCTTACAGTTGAAAAGGTTTGATTTTGATTA TACAACCATGCATAGAATTAAACTGAATGATCGAATGTCATTTCCGGAGGAGCTGGATATGAGCACATTT ATTGATATTGAAGATGAGAAATCTCCTCAGACTGAAAGTTGCACTGATAGTGGAGCAGAAAATGAAGGCA GTTGCCACAGCGACCAGATGAGTAATGATTTCTCCACGGATGATGCTGTTGATGAAGGGATATGCCTGGA GAGCAGTAGTGGGAGTGAGAAGATTTCAAAACCTGGCCTGGAAAAGAACTCCCTGATGTATGAGCTCTTC TCAGTGATGGTGCATTCAGGGAGTGCTGCTGGAGGACACTATTATGCTTGCATAAAGTCTTTCAGTGACG ATCAATGGTACAGTTTCAATGACCAACATGTGAGCAGGATAACACAAGAAGACATTAAGAAGACACATGG TGGGTCTTCAGGAAGCAGAGGCTACTACTCCAGTGCTTTTGCAAGCTCAACAAATGCATATATGCTGATA TACAGACTGAAGGATCCAACAAGAAATGCAAAGTTTCTAGAAGTGGACGAGTACCCAGAACACATTAAGA ATCTGGTGCAGAAGGAGAGAGAGCTGGAAGAGCAGGAAAAGAGGCAACGAGAGATTGAACGCAACACGTG CAAGATAAAATTATTTTGTTTGCATCCTGTGAAACAAGTAATGATGGAAAACAAGCTGGAGGTTCATAAG GATAAGACGCTAAAGGAAGCCGTTGAAATGGCTTATAAGATGATGGATTTAGAAGATGTGATTCCCTTGG ATTGCTGTCGCCTGGTAAAATATGACGAGTTTCATGATTACCTGGAACGATCGTATGAGGGAGAAGAAGA TACACCAATGGGGTTGCTTCTAGGAGGAGTCAAGTCCACATACATGTTTGATCTGCTGTTGGAAACGAGA AAGCCTGATCAAATTTTTCAGTCTTACAAACCTGGAGAAGTGATGGTGAAAGTTCATGTTGTTGATCTGA AAGCAGAGACTGTCGCTGCTCCTGTAACAGTGCGTGCGTATTTAAATCAGACAGTTACAGAGTTTAAACA GCTGATCTCAAAGGCCACGCACTTACCTGCAGACTCCATGAGGATAGTGCTGGAGCGCTGCTACAATGAC CTGCGCCTTCTCAGCATGCCCAGCAAGACCCTGAAGGCTGAGGGCTTCTTCAGAAGTAACAAGGTGTTTG TTGAAAGCTCTGAGACAGTGGATCACCAGGCAGCCTTTACAGACTCCCACTTGTGGAAACTCCTGGATCG ACATGCAAATACAATCCGGTTATTTGTTTTGCTGCCTGAACAATCTCCAGGATCATATTCGAAAAGGACA GCATACCAGAAAGCTGGAGGTGATTCTGGTAATGTGGACGATGATTGTGAAAGAGTCAAAGGACCTGCAG GGAATTTAAAGTCTGTGGATGCTATTCTAGAAGAGAGCACTGAAAAACTGAAAAGCTTGTCACTGCAGCA GCAGCAGCAAGATGGGGACAACGGAGACAGCAGCAAAAGCACTGAGACAAGTGACTTTGAAAATATTGAA TCCCCTCTCAATGAGAGGGGCTCTTCCACCTCTGTGGATAATCGAGAACTTGAACAGCACATTCAGACTT CTGACCCAGAAAACTTTCAGTCTGAGGAACGCTCGGACTCAGATGTGAATAATGACCGGAGTACAAGTTC GGTAGACAGTGATATTCTTAGCTCCAGTCACAGCAGTGACACTTTGTGCAATGCAGACAGCGCTCAGATT CCTCTGGCCAATGGACTTGACTCTCACAGTATCACAAGTAGTAGAAGAACTAAAGCAAACGAGGGGAAAA AGGAGACCTGGGACACAGCTGAAGAAGACTCTGGGACTGACAGTGAATATGACGAGAGTGGCAAGAGCAG GGGGGACATGCAGTACATGTACTTCAAAGCTGACCCCTACACTGCGGATGAAGGCTCTGGGGAAGGACAC AAATGGTTGATGGTGCATGTTGATAAGAGAATCACCCTGGCAGCTTTCAAACAGCATTTAGAACCTTTTG TTGGAGTCCTCTCCTCTCACTTCAAGGTTTTTCGAGTGTACACCAGCAATCAAGAGTTTGAGACCGTCCG GTTGAATGAGACCCTTTCATCATTTTCAGATGACAATAAGATCACAATCCGACTGGGGAGAGCACTGAAG AAGGGAGAGTACAGAGTTAAAGTGTGCCAGCTCTTAGTCAATGAGCAAGAGCCATGCAAGTTTCTGCTAG ACGCTGTGTTTGCTAAAGGCATGACTGTGCGGCAATCAAAAGAGGAGCTGATTCCTCAACTCAGGGAGCA GTGTGGCTTAGACCTCAGCATCGACAGGTTTCGTCTAAGGAAGAAAACATGGAAGAATCCTGGCACTGTC TTTTTGGATTATCATATTTATGAAGAAGATATTAATATTTCCAGCAACTGGGAAGTTTTCCTGGAAGTTC TTGATGGTGTGGAGAAGATGAAGTCCATGTCACAGCTTGCCATCTTAACAAGACGCTGGAGGCCTGCAGA GATGAAGTTGGACCCCTTCCAGGAGCTTGTGTTGGAAAGCAATAGTGTTGATGAACTCCGAGAGAAGCTA AGTGAAATCAGTGGGATTCCTTTGGAAGACATTGAATTTGCCAAGGGTAGGGGAACCTTCCCCTGTGATA TTTCTGTCCTTGATATTCATCAAGATTTGGACTGGAATCCTAAAGTCTCTACCCTGAATGTTTGGCCTCT TTATATCTGTGATGATGGTGCCGTCATATTTTATAGGGATAGAACAGAAGAAGTGATGGAATTGACAGAT GAGCAAAGAAATGAATTGATGAAAAAGGAAAGCAGCCGACTGCAGAAGACGGGACATCGTGTGACATACT CCCCTCGCAAAGAGAAAGCACTCAAGATCTACCTGGATGGGGCGCCAAATAAAGATGTGGCTCAAGACTG A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_177249
Insert Size	4131 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_177249.3, NP_796223.2
RefSeq Size	5600 bp
RefSeq ORF	4131 bp
Locus ID	74996
UniProt ID	Q8BY87
Gene Summary	Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER) (By similarity). Acts as a regulator of cell growth and genome integrity. May also indirectly regulate CDC25A expression at a transcriptional level.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 3 encode protein isoforms of identical length but with distinct amino acid sequences.

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