Tmem2 (NM_001033759) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3110012M15Rik; mKIAA1412
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229573 representing NM_001033759 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGCCGCTGGTTCCAGGGGCCACTCCCCTGCTTTCCTGCAACCTCAAAACGGAAACGGCCACCGCT CACCTGGTTATGTTCCCGGGAAGGTGGTTCCCTTACGTCCCGCGCCTCCTCCAAAGAACCACGCCTCCGC CAAACTGACCTCCAGGAGCCAAGACGCCCCAGCGACCTTTGCTTTCTCCCCCGAGGAGCAGCGGACTCCG AGCGAGAGCCGAAAGCGGAAGAGGCACAAGAACACCTTCATCTGCTTCGCCATAACCAGCTTCTCGTTCT TTGTGGCGCTTGCGGTCATTTTGGGAATATCCTCAAAATACGCTCCAGATGAGAATTGTCCAGATCAAAA CCCTCGTCTCAGGAACTGGGATCCAGGACAAGATTCTGCGAAGCACATTGTTATCAAGGAGGGGGATTTG TTCCGCCTGACCTCAGATGCAACAGTGGACTCTATAGTCATTCAGGATGGAGGACTGCTTGTGTTTGGGG ATGATAAAGATGGATCCAAGAATATAACTTTGAGGACTCGATACATCCTGATCCAGGATGGTGGGGCGCT TCATATTGGAGCAGAAAAATGCCGCTATAGATCCAAAGCGACAATTACCTTGTATGGCAAGTCAGATGAA CGTGAAAGTATGCCAATATTTGGCAAGAAGTTTATTGGCGTGGAGGCTGGCGGGACACTGGAGTTACACG GGGCACAGAGGACATCATGGACGATGCTGGCGAGGACTCTGCATTCTTCAGGCTTGCCCTTTGGGTCTTA TGCCTTCGAGAAGGACTTTTCCCGGGGCCTTAATGTGAGGGTCATTGATCAAGACACAGCCAGAGTTTTA GAAAACGAGAAGTTCGATACCCACGAGTACCACAATGAGAGCAGGAGGCTGCAGGAGTTTTTGAGAGCTC AGGAGCCCGGCCGGATTGTGGCTATTGCTGTTGGAGACTCAGCAGTCAAGAGCCTGTTACAGGGAACAAT CCAGATGATCCAGGATCGGTTGGGGAGTAAACTGATCCAAGGTCTGGGCTACAGGCAAGCTTGGGCCTTA GTTGGTGTCATCGATGGTGGAAGTTCTTCCTGCAATGAATCTGTGAGAAACTATGAAAACCATAGCACTG GGGGGAAGGCCCTGGCTCAAGGAGAATTCTACACCCTGGACGGTCAGAAGTTCTCTGTGACAGCCTACAG TGAATGGAGTCAAGGAATCTCCCTCTCAGGCTTCCGGGTGGACATTGCGGATGGAGTGAAGCTTCACCTG TTGGACGACGTCAGCACTTGGGAAGCTGGAGACCGAATTGTGGTGGCCAGCACCGACTATTCCATGTACC AAGCAGAGGAGCTAACTCTTCTCCGCTGCCCCGAGTGCAGCCGTTCTCAGGTCAAAGTCAAAGAGATCCC CCAGTACCTGCACGTCGGTGAGATCATAGACGGGATAGACATGAGAGCTGAGGTTGGACTTCTCACCCGC AACATTGTGATCCAAGGAGAGATGGAGGATTCATGCTATGCAGAAAATCACTGCCAGTTCTTCGATTATG ACACCTTTGGGGGACACGTCATGATAGAGAAGAATTTTACTTCAGTCCATCTTTCTTATGTGGAATTGAA ACACATGGGCCAGCAGCATATGGGCCGGTATCCTGTGCATTTTCACCTGTGTGGAGATGTAGATTCTAAA GGAGGGTACAGCCAGCCAGCGTCTGTGGATGGCCTGTCTGTTCATCACAGCTTTTCAAGGTGCATCACCG TGCACGGGACCAGTGGCTTGCTAATCAAAGACACCATTGGCTTCGACACACTAGGTCATTGCTTCTTTTT GGAAGATGGTGTTGAACAGCGAAATATTTTGTATCATAACTTGGGGCTTCTTACCAAGCCGGGCACTCTC CTCCCAACGGACAGGAACAGCTCCATGTGCACCGTCATGCGTGATGGAGTGTTTGGAAACTACGTCCCTG TGCCTACCACAGACTGCATGGCTGTTTCAACTTTCTGGATTGCCCACCCCAACAACCACCTGATCAATAA CGCAGCTGCAGGCTCACAGGATGCTGGAATATGGTATTTATTCCACAAGGAACCAACTGGGGAGTCCAGT GGGCTGCAGCTCCTGGAGAAGCCAGAACTCACTCCGCTGGGGATATTTTATAACAACAGGGTTCATTCAA ATTTTAAGGCTGGTTTATTTGTGGACAAAGGTGTCAAAACGACCAACGCCAGTGCATCTGACCCAAGGGA GTACCTCTGCCTGGATAACAGTGCAAGGTTTCGGCCCCATCAGGATGCAGACCCTGAGAAGCCACGCGTT GCAGCCATCATCGACAGGCTCATTGCTTTTAAGAACAATGACAACGGAGCCTGGGTCAGAGGAGGAGATA TTATTGTTCAGAATTCAGCATTTGCAGATAACGGAAAGGGGCTGACCTTTGCCAGTGATGGGAGCTTCCC AAGCGATGAAGGGTCCAGTCAGGAGGTGACAGAGTCCCTCTTTGTCGGGGAGAGTAGGAATTACGGCTTT CAAGGTGGCCAAAACAAGTACATGGGCACAGGGGGAATCGATCAGAAGCCTCGGACGCTACCCAGGAACA GGACATTCCCAATCCGAGGCTTTCAGATTTACGATGGGCCTATTCATCTCACAAAGAGTACTTTCAAAAA GTATGTGCCAACTCCAGACAGGTACAGCAGTGCAATTGGCTTCCTCATGAAGAATTCCTGGCAGACAACA CCCAGGAATAACGTTTCCCTTGTGAAGTTTGGTCCACAGGTCTCTCTGAACGTCTTCTTTGGAAAGCCTG GCCCCTGGTTTGAGGATTGTGAGCTGGACGGCGATAAGAACTCCATCTTCCATGACATTGATGGCTCTGT GACAGGATACAAGGACACCTATGTGGGAAGGATGGACAACTACCTGATCCGCCATCCAAACTGTGTCAAT GTTACTAAGTGGAATGCAGTGATCTGTAGCGGGACCTACGCCCAGGTGTATGTTCAGACCTGGAACACTC CGAATCTTAGCATGATCATCACACGGGATGAATACCCATCCCACCCAATGGTGCTTCGGGGTATCAACCA GAGGGCCATCTCTCCACAGTACCAGCCTGTTGTCATGCTGGAGAAGGGCTACACCATCCACTGGAATGGT CCAGCGCCACGAACCACTTTCTTGTACCTCGTGAACTTCAACAAGGATGACTGGATCCGGGTTGGCCTCT GTTACCCAGCCAACACGAGCTTTCAGGTTACCGTTGGCTTTCTGCAGCGGCAGAACGGCTCATTATCTAG AATTGAGGACTATGAGCCTGCGCGGTCGATGGAGGAACTGCAGAAGAAGCCGTCTGAGAGAAAATTCTAT TTTGACTCTGGCACAGGGTTATTGTTTCTGTATCTCAGAGCCCACAGCCACAGAGATGGCCACAGTTACT GTTCATCTCAGGGATGTGAAAGAGTCAAGATTCAGGCAGCAACAGACTCCAAAGATATCAGTAACTGCAT GGCCAAAGCGTACCCGCAGTATTACAAGAAGCCCTCGGCGGTCAAGCGCATGCCGGCCATGCTCACGGGG CTCTGTCAAGGCTGTGGAACCCACCAGATGGTGTTTACCAGTGACCCTCACAAAAGCTACCTCCCTGTAC GATTCCAGTCACCTGGGAAAGCAGAAATTCAGCGAGGAGATCCATCCATTATTTCCGTCAATGGTACTGA CTTTACCTTCCGGAGTGCGGGTGCCCTCCTCCTCATTGTGGATGCCTGCAGTGTGCCCTTCCGGGTGAAG GAAAAGCGGATGTTCCTTTCTGCAGACGTCAGTCACATGGAAGAATATTTCAAGGCTAGCATCCCCCCAA GGTCAATCGTTCTGTTGAGCACAAGAGGAGAAATTAAGCAGCTGAATATCTCGGATTCATTAGCAGTTCT GGGACTGGCCAAGCCAGCACATCTGTACAGCAAAGGTAGTGTCGTATTTTTGGGATTCAGCGGAAACTTC GCACCGTCATGGACGAAGCTGTTCACCAGTCCCGATGAGCAGGGCCTCGGAGTGCTTGAGCAGTTTCTCC CGTTGCAGATGGAGGAATATGGGTGCTCCCGGACCGGCAGTGTCCACAGAAGAGACCTTGACCTGTTACA GCAGGCTTTGAAAGTGCTTTAG ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-NotI
ACCN	NM_001033759
Insert Size	4152 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001033759.2, NP_001028931.1
RefSeq Size	6620 bp
RefSeq ORF	4152 bp
Locus ID	83921
UniProt ID	Q5FWI3
Gene Summary	Cell surface hyaluronidase that mediates the initial cleavage of extracellular high-molecular-weight hyaluronan into intermediate-size hyaluronan of approximately 5 kDa fragments (By similarity). Acts as a regulator of angiogenesis and heart morphogenesis by mediating degradation of extracellular hyaluronan, thereby regulating VEGF signaling (By similarity). Is very specific to hyaluronan; not able to cleave chondroitin sulfate or dermatan sulfate (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein.

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