Lrrc7 (NM_001291452) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AK122498; densin; mKIAA1365
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229604 representing NM_001291452 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTGCTTGGAGATGACCACCAAACGGAAGCTCATTGGCCGCCTGGTGCCATGCCGCTGCTTCCGTG GAGAGGAAGAGATCATCTCAGTGTTGGATTACTCCCACTGCAGCCTGCAACAGGTGCCCAAGGAGGTCTT CAACTTCGAACGCACACTAGAAGAGCTCTATCTGGATGCCAATCAGATTGAGGAGCTACCTAAGCAATTG TTCAACTGTCAAGCTCTACGGAAACTAAGCATTCCTGATAATGACCTCTCAAGTCTGCCAACCTCCATTG CTAGCCTAGTTAACCTGAAAGAACTCGACATCAGTAAAAACGGTGTACAAGAATTTCCAGAAAACATAAA GTGCTGTAAGTGTTTAACCATAATTGAAGCCAGTGTCAATCCCATTTCTAAACTACCCGATGGCTTCACG CAGCTTCTTAACCTGACCCAGCTCTACCTGAATGACGCATTCCTCGAATTTCTTCCAGCTAATTTTGGAA GACTTGTCAAATTGCGAATACTGGAGTTAAGAGAAAATCACTTGAAAACTCTACCAAAGTCAATGCACAA GTTAGCCCAGCTGGAAAGACTTGACCTAGGCAATAATGAATTCAGCGAGCTGCCTGAAGTTCTGGATCAA ATACAGAATTTGAGGGAGCTGTGGATGGATAACAATGCATTGCAAGTGCTGCCTGGGTCTATAGGGAAGT TAAAGATGTTGGTATACCTGGATATGTCAAAAAACAGGATAGAAACGGTTGATATGGATATTTCTGGATG TGAAGCCCTCGAGGACCTCTTACTATCATCCAATATGTTGCAACAGCTGCCTGACTCAATAGGATTGCTG AAAAAGTTAACAACTCTAAAAGTAGATGACAATCAACTTACAATGCTACCCAACACAATTGGAAACTTAT CTTTATTAGAAGAATTTGACTGCAGCTGCAACGAGCTGGAGTCCCTCCCTCCCACCATTGGTTACCTGCA CAGCCTTCGAACATTAGCGGTTGACGAGAATTTCCTCCCAGAGTTGCCAAGAGAAATTGGAAGCTGTAAG AATGTCACCGTTATGTCTCTACGCTCCAATAAGCTAGAATTTCTTCCTGAAGAAATTGGGCAGATGCAGA GACTGCGTGTCTTAAATCTAAGTGATAATAGGTTGAAGAATTTGCCATTCTCTTTCACCAAACTCAAAGA GCTTGCAGCATTGTGGCTTTCTGACAATCAGTCCAAGGCCCTTATCCCTCTGCAAACAGAAGCCCACCCA GAAACCAAGCAAAGGGTACTAACCAACTACATGTTTCCCCAGCAGCCCCGTGGTGACGAAGATTTCCAGT CAGACAGCGACAGCTTCAACCCCACCCTGTGGGAGGAGCAGAGACAACAGCGCATGACTGTTGCCTTTGA ATTTGAGGATAAAAAAGAAGATGACGAAAGTGCTGGGAAAGTTAAGGCTCTCTCCTGCCAAGCCCCCTGG GACAGGGGCCAGCGTGGGATTACTCTCCAACCTGCCAGGCTGTCTGGCGATTGCTGCACACCATGGGCCA GGTGTGATCAGCAGATCCAAGATATGCCCGTCCCCCAGAGTGACCCCCAGCTGGCATGGGGTTGTATAAG TGGCCTCCAGCAGGAGAGGAGCATGTGTGCTCCATTGCCAGTGGCAGCACAGTCCACCACTCTTCCCTCT CTAAGTGGCAGACAGGTTGAAATAAACCTAAAACGATATCCAACTCCTTACCCGGAGGATTTAAAGAATA TGGTAAAATCTGTTCAAAATCTGGTGGGTAAGCCAAGCCATGGAGTGCGTGTTGAGAACTCAAATCCAAC AGCTAACACGGAGCAAACTGTGAAAGAAAAGTTTGAACACAAGTGGCCGGTGGCCCCAAAGGAGATTACA GTGGAGGATTCTTTTGTTCATCCAGCTAATGAAATGAGGATTGGGGAACTTCACCCTTCATTAGCTGAGA CCCCTCTGTACCCACCCAAACTTGTTCTGCTAGGGAAGGACAAAAAAGAATCAACTGATGAGTCTGAAGT TGACAAAACTCACTGTCTGAATAACAGTGTCTCCTCAGGCACCTACTCAGACTACTCGCCCTCCCAAGCT TCCTCGGCATCCTCTAACACCCGGATGAAAGTGGGGTCCTTGCAGGCAACAGCTAAAGATGCCGTCCATA ACTCTTTGTGGGGTAACAGGATTGCACCACCTTTCCCACAGCCTCTAGATGCAAAGCCATTACTCAGCCA ACGGGAGGCTGTCCCTCCAGGGAACATCCCCCAGCGTCCTGATAGGCTGCCAATGAGTGATGCTTTCCCT GACAACTGGACTGATGGCTCCCATTACGACAACACTGGTTTTGTCTCCGAGGAAGCCGCAGGCGAAAATG CCAACAATAACCCTCTCTTAAGTTCCAAGGCTAGAAGCGTACCTGCTCATGGGCGCAGGCCTTTAATTAG GCAAGAAAGGATTGTCGGTGTTCCCCTGGAACTGGAGCAGTCCACACACAGACACACACCAGAAACAGAA GTGCCTCCTTCCAATCCCTGGCAGAATTGGACCAGAACTCCCAGTCCATTTGAAGATAGGACTGCTTTTC CTTCCAAATTAGAAACAACCCCTACTACCAGTCCCTTGCCTGAAAGAAAAGACCACATGAAGGAGCCTAC AGAGACACCAGGTCCCTTTTCTCCGGGAGTGCCATGGGAATACCACGACCCCACCCCTAACAGGAGTCTC GGTAATGTCTTTTCTCAAATCCACTGCCGCCCAGATTCGTCTAAAGGTGTTATCGCAATTAGCAAAAGCA CAGAGAGGCTTTCCCCGCTCATGAAAGATATAAAGTCCAACAAGTTCAAAAAGTCCCAGAGTATTGATGA GATTGACGTCGGTACCTACAAGGTCTATAACATTCCACTAGAAAACTATGCCTCTGGGAGCGATCACTTA GGAAGCCATGAGCGGCCAGACAAGTTCTTGGGACCAGAGCATGGCATGTCCAGTATGTCCAGGAGCCAGT CAGTACCCATGTTGGATGACGAGATGCTCATGTATGGAAGTAGTAAAGGCCCACCCCAACAAAAAGCGTC TATGACTAAGAAGGTCTATCAGTTTGACCAAAGCTTCAACCCACAAGGAGCGGTGGAAGTAAAAGCCGAG AAGAGGATTCCACCCCCTTTTGCACACAATTCTGAGTATGTACAGCAGCCCAGTAAAAACATCGCCAAGG ATCTGGTCAGCCCCAGAGCCTACAGAGGATACCCACCCATGGAACAGATGTTCTCCTTTTCCCAGCCATC TGTAAATGAGGATGCCATGGTGAATGCTCAGTTCGCCAGCCAGGGGCCCAGGGCAGGCTTCCTAAGGAGG GCTGACTCCCTGGCCAGCTCCACCGAGATGGCCATGTTCAGAAGGGTCAGTGAGCCCCACGAGCTGCCCC CTGGTGACAGGTATGGCCGAGCAACCTATAGGGGTGGGCTGGAAGGCCAGAGCAGCATTTCAATGACTGA TCCCCAATTCCTCAAGAGGAATGGCAGGTACGAAGATGAGCACCCTTCATATCAAGAAGTGAAAGCGCAG GCGGGAAGTTTTCCTGCTAAAAACCTTACCCAGAGGAGGCCGCTGTCTGCCAGGAGCTACAGCACAGAGA GTTATGGGGCCTCCCAAACCAGGCCAGTTTCAGCTAGGCCTACCATGGCAGCCCTCCTGGAAAAAATACC ATCTGACTATAACTTGGGTAACTATGGTGACAAGACATCAGATAACAGTGATATAAAGACAAGGCCCACC CCTGTGAAGGGAGAGGAGAGCTGTGGCAAAATGCCGGCAGACTGGAGACAGCAGCTGCTTAGACATATAG AAGCTAGAAGGTTAGACAGGACCCCGTCCCAGCAAAGCAACATTTTAGACAATGGACAAGAAGATGTCTC TCCTAGTGGCCAATGGAATCCTTACCCACTTGGGAGGCGGGATGTACCTCCGGACACCATTACTAAAAAG GCAGGCAGCCACATTCAGACCCTGATGGGGTCACAGAGCCTTCAGCACCGCAGCCGGGAGCAGCAGCCAT ATGAAGGGAACATCAACAAAGTGACCATTCAGCAGTTCCAATCGCCATTGCCCATCCAGATCCCCTCATC ACAGGCTACCCGGGGACCTCAGCCTGGACGGTGCTTAATTCAAACGAAAGGGCAGAGGAGTATGGACGGT TATCCCGAGCAGTTCTGTGTGAGGATAGAAAAGAATCCTGGGCTTGGATTTAGTATCAGCGGTGGAATTA GTGGGCAAGGAAATCCTTTCAAACCTTCTGACAAGGGTATCTTTGTTACTAGGGTTCAGCCTGATGGACC AGCATCCAACCTACTACAGCCCGGGGATAAAATCCTTCAGGCAAATGGACACAGTTTTGTGCATATGGAA CATGAAAAGGCTGTTTTACTACTGAAGAGCTTCCAGAACACAGTAGACCTAGTTATTCAACGTGAGCTCA CTGTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291452
Insert Size	4488 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291452.1, NP_001278381.1
RefSeq Size	7321 bp
RefSeq ORF	4488 bp
Locus ID	242274
UniProt ID	Q80TE7
Gene Summary	Required for normal synaptic spine architecture and function. Necessary for DISC1 and GRM5 localization to postsynaptic density complexes and for both N-methyl D-aspartate receptor-dependent and metabotropic glutamate receptor-dependent long term depression.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1.

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