Nrxn3 (NM_001198587) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229632 representing NM_001198587 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTTTACCCTCCACTCAGTTTTCTTCACCTTGAAGGTGAGCATCTTTCTGGGCTCCCTGGTGGGGC TTTGCCTGGGTCTGGAGTTCATGGGCCTTCCTAACCAGTGGGCCCGCTACCTGCGTTGGGATGCAAGCAC GCGCAGTGACCTGAGCTTCCAGTTCAAGACCAATGTTTCCACTGGGCTGCTCCTGTATTTGGATGATGGT GGTGTCTGTGACTTCCTCTGTCTCTCCCTGGTGGATGGCCGCGTTCAGCTCCGCTTTAGCATGGACTGTG CCGAGACCACTGTGCTATCCAACAAGCAGGTGAACGACAGCAGTTGGCACTTCCTCATGGTGAGCCGTGA TCGTGTGCGCACTGGGCTGGTGATTGATGGTGAGGGCCAGTCTGGAGAGCTACGGCCCCAGCGGCCTTAT ATGGATGTGGTCAGTGATTTGTTCCTCGGTGGCGTCCCCGCTGACATTCGACCTTCTGCCCTGACCCTCG ATGGAGTACAGAGCATGCCTGGCTTTAAGGGATTAATGCTGGATCTCAAGTATGGCAACTCGGAACCTCG GCTTCTGGGGAGCCAGAGCGTCCAGTTAGAAGCAGAGGGACCCTGTGGCGAGCGTCCCTGTGAAAATGGT GGGATCTGCTTCCTCTTGGATGGTCATCCCACCTGTGACTGTTCTACCACTGGCTATGGTGGCACACTCT GCTCAGAAGATGTCAGTCAAGGTCCAGGCCTCTCCCATCTTATGATGAGTGAACAAGCACGAGAGGAGAA CGTGGCCACCTTCCGAGGCTCAGAGTATCTGTGCTATGACCTGTCCCAGAACCCCATCCAGAGCAGCAGC GACGAAATCACCCTTTCCTTTAAGACCTGGCAACGCAATGGGCTCATCCTCCACACTGGCAAGTCGGCCG ACTATGTTAACCTGGCTCTGAAGGATGGTGCGGTCTCCTTGGTCATTAACCTGGGGTCCGGGGCCTTTGA GGCCATTGTGGAGCCAGTGAATGGGAAATTCAACGACAACGCCTGGCATGATGTCAAAGTGACACGCAAT CTTCGGCAGGTGACAATCTCTGTGGATGGCATCCTAACCACAACGGGCTACACTCAAGAGGACTACACCA TGCTGGGCTCAGATGACTTCTTCTATGTTGGTGGAAGCCCAAGTACCGCAGACTTGCCAGGTTCACCTGT GAGCAACAACTTCATGGGCTGCCTTAAAGAGGTTGTTTATAAGAATAATGACATCCGTCTAGAGCTGTCT CGCTTGGCCCGGATTGGAGACACCAAGATGAAAATCTATGGTGAAGTTGTGTTCAAGTGTGAGAACGTGG CCACACTGGATCCCATCAACTTTGAGACCCCAGAGGCTTACATCAGCTTGCCCAAGTGGAACACCAAACG TATGGGCTCCATTTCCTTTGACTTTCGCACCACTGAGCCCAACGGCCTGATTCTCTTCACTCACGGGAAG CCTCAAGAAAGGAAAGATGTCCGGAGCCAAAAGAACACAAAAGTTGACTTCTTTGCTGTGGAACTTCTTG ATGGCAACCTGTATTTGCTGCTTGACATGGGCTCTGGCACCATCAAAGTGAAGGCCACTCAGAAGAAGGC CAATGATGGGGAATGGTACCACGTGGATATTCAGCGTGATGGCAGATCAGGTACCATATCTGTGAACAGC AGGCGCACGCCATTCACCGCCAGTGGGGAGAGTGAAATTCTCGACCTGGAGGGGGACATGTATCTGGGCG GGCTGCCAGAGAACCGAGCTGGCCTCATCCTCCCCACGGAGCTCTGGACCGCCATGCTCAACTACGGTTA CGTGGGCTGCATCCGCGACCTGTTCATCGATGGGCGCAGCAAGAACATCCGGCAGCTGGCGGAGATGCAG AACGCAGCAGGCGTCAAGTCCTCCTGTTCACGCATGAGCGCCAAGCAGTGTGACAGCTACCCCTGCAAGA ACAACGCGGTGTGCAAGGACGGCTGGAACCGCTTCATCTGCGACTGCACTGGCACCGGCTACTGGGGGAG AACCTGCGAAAGAGAGGCATCTATCCTGAGCTATGACGGCAGCATGTACATGAAGGTCATCATGCCCATG GTGATGCACACGGAGGCAGAGGATGTGTCCTTCCGCTTCATGTCACAGCGAGCCTATGGGCTACTGGTAG CCACAACCTCCAGGGATTCTGCAGATACACTGCGTCTAGAGTTGGATGGTGGGCGTGTCAAGCTCATGGT TAACTTAGACTGTATCAGGATAAACTGTAACTCCAGCAAAGGACCTGAGACCCTGTATGCAGGGCAGAAG CTCAATGACAATGAGTGGCACACCGTCCGGGTAGTGCGGAGAGGAAAAAGCCTTAAGCTAACAGTGGATG ATGATGTGGCTGAGGGGACAATGGTGGGCGACCACACCCGCCTGGAATTCCACAATATTGAAACTGGTAT CATGACTGAGAAGCGTTACATCTCTGTGGTCCCCTCCAGTTTCATTGGCCATCTACAGAGCCTCATGTTC AATGGCTTACTGTACATTGATTTGTGCAAAAATGGCGACATCGACTACTGTGAACTGAAGGCTCGCTTTG GACTGAGAAACATCATCGCCGACCCTGTCACTTTTAAGACCAAGAGCAGCTACCTGACCCTCGCCACCCT ACAGGCTTACACCTCCATGCACCTCTTCTTCCAGTTCAAGACCACTTCAGCTGATGGCTTCATTCTCTTC AATAGCGGAGATGGCAATGATTTTATTGCAGTTGAGCTGGTCAAGGGGTACATACACTATGTGTTTGATC TCGGCAATGGTCCCAATGTGATCAAAGGCAACAGTGACCGCCCCCTGAATGACAACCAGTGGCACAACGT GGTCATCACCAGGGACAGCAGTAACACCCACAGTCTGAAGGTGGACACCAAGGTAGTCACTCAGGTCATC AATGGTGCCAAAAATCTGGATTTGAAAGGTGACCTCTATATGGCTGGCTTAGCCCAGGGCATGTACAGCA ACCTTCCCAAGCTTGTGGCCTCCAGGGATGGATTTCAGGGCTGCCTGGCCTCTGTGGACTTGAATGGACG CCTGCCTGATCTCATCAACGATGCTCTCCACAGGAGTGGACAGATCGAGCGAGGCTGTGAAGGACCCAGT ACAACCTGTCAGGAGGATTCATGCGCCAACCAGGGAGTTTGCATGCAGCAGTGGGAAGGTTTCACCTGTG ACTGCTCCATGACATCATATTCTGGAAACCAGTGCAATGACCCTGGTGCAACATACATCTTTGGGAAAAG CGGTGGCCTCATCCTCTATACCTGGCCAGCAAATGACAGACCCAGCACACGCTCTGACCGTCTCGCCGTG GGCTTCAGCACTACTGTGAAGGATGGTATCTTAGTACGCATTGACAGCGCCCCTGGACTTGGCGACTTCC TCCAGCTTCACATAGAACAAGGGAAAATTGGAGTTGTCTTCAATATTGGCACAGTTGACATCTCCATCAA AGAAGAGAGAACTCCTGTCAATGATGGCAAATACCACGTTGTGCGCTTCACCAGGAATGGGGGAAATGCT ACACTTCAGGTGGACAACTGGCCAGTGAATGAGCACTATCCTACAGGCAACACTGATAATGAACGCCTCC AAATGGTAAAACAGAAAATCCCCTTCAAATATAACCGGCCCGTAGAGGAGTGGCTGCAGGAAAAAGGCCG GCAGCTAACCATCTTCAACACCCAGGCGCAAATAGCCATCGGAGGAAAGGACAAAGGACGTCTCTTCCAA GGTCAACTCTCTGGGCTCTATTATGATGGTTTGAAAGTACTGAACATGGCAGCTGAGAACAACCCTAATA TTAAAATCAATGGAAGTGTCCGGCTAGTGGGAGAAGTCCCATCAGTCTCAGGAACAACACAGACAACGTC CATGCCACCTGAAATGTCTACCACCGTCATGGAAACCACCACCACCATGGCTACGACCACCACCCGAAAG AACCGCTCTACAGCCAGCATTCAGCCCACGTCAGATGATCTTGTTTCATCTGCTGAATGTTCAAGTGATG ATGAAGACTTTGTCGAATGTGAACCAAGTACAGATAAGAGTCTTTCCACTTCAATCTTCGAAGGTGGCTA CAAAGCACATGCGCCCAAGTGGGAATCCAAGGACTTTAGACCTAACAAAGTCTCGGAAACTAGTAGAACT ACAACCACCTCTTTGTCCCCTGAGCTGATCCGCTTCACAGCGTCCTCCTCGTCTGGGATGGTGCCCAAAT TGCCAGCTGGCAAAATGAATAACCGTGATCTCAAACCCCAGCCTGATATAGTCTTGCTTCCGTTGCCCAC TGCCTATGAGCTAGACAGCACCAAACTGAAGAGCCCACTAATTACTTCCCCCATGTTCCGTAATGTGCCC ACAGCAAACCCCACGGAGCCAGGAATCAGACGGGTTCCGGGGGCCTCAGAGGTGATCCGGGAGTCCAGCA GTACAACAGGGATGGTCGTCGGCATTGTGGCTGCTGCCGCCCTCTGCATCTTGATCCTCCTGTACGCCAT GTACAAGTACAGGAACAGGGACGAGGGGTCCTATCAAGTGGACGAGACGAGGAACTACATCAGCAACTCG GCCCAGAGCAACGGCACGCTCATGAAGGAGAAGCAAGCCAGCTCCAAGAGCGGCCATAAGAAACAGAAAA ACAAGGACAAGGAGTATTATGTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001198587
Insert Size	4716 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001198587.3, NP_001185516.2
RefSeq Size	7933 bp
RefSeq ORF	4716 bp
Locus ID	18191
UniProt ID	Q6P9K9
Gene Summary	This gene encodes a member of a family of proteins that function in the nervous system as receptors and cell adhesion molecules. Extensive alternative splicing and the use of alternative promoters results in multiple transcript variants for this gene, but the full-length nature of many of these variants has not been determined. Transcripts that initiate from an upstream promoter encode alpha isoforms, which contain epidermal growth factor-like (EGF-like) sequences and laminin G domains. Transcripts initiating from the downstream promoter encode beta isoforms, which lack EGF-like sequences. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). This variant encodes an alpha isoform of neurexin 3.

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