Dock10 (NM_001285927) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9330153B10Rik; A630054M16Rik; Jr4; Jr5; mKIAA0694; R75174; ZIZ3; Zizimin3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229716 representing NM_001285927 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAGCTTCCGAGGAAAGGAATTCTGGAAGAGGAGGCGAACAGTGAAGAGAGTGAACCCAGAAGGAA TCCACAAGGCTGGTGCTCAGGAAAAGCCCAGACTCTTGGATCCTTTGGATTATGAAACCGTCATTGAAGA ACTTGAAAAGACCTATCGGGATGATCCTCTTCAGGACCTTCTGTTCTTCCCCAGTGATGACTTCTCAACA GCCACGGTTTCTTGGGATATCCGAACACTCTATTCGACGGTACCAGAAGAGGCAGAGCACAGGGCAGAAA GTCTGCTGGTGAAAGAGGCTTGTAAATTCTACAGTTCCCAGTGGTACGTGGTAAACTACAAATATGAACA ATATTCTGGAGATATTCGACAGTTGCCCCGAGCAGAACACAAGCCAGAGAAACTTCCTTCACATTCCTTT GAGGTTGACCATGAAGATGCTGATAAGGATGAAGATACCACATCCCACTCATCATCCAAGGGCGGTGGGG GAGCTGGAGGTACTGGTGTGTTCAAGTCTGGCTGGCTTTACAAGGGGAATTTTAACAGCACTGTGAACAA TACTGTCACTGTTCGGTCATTCAAAAAGCGCTACTTCCAGCTGACTCAGTTGCCTGATAACTCCTACATT ATGAACTTTTACAAAGATGAAAAGATATCCAAAGAGCCCAAGGGCTGCATCTTTTTGGATTCCTGCACAG GTGTAGTACAGAATAACAGGCTGAGGAAATATGCCTTCGAGTTGAAGATGAACGACCTGACCTATTTTGT GCTGGCTGCTGAAACAGAGTCAGATATGGACGAATGGATCCACACACTTAACCGTATCCTGCAGATCAGT CCCGAGGGCCCACTCCAGGGAAGGAAAAGTGCAGAGCTTGCTGAGCTGGGGCTAGATCCACTGGATAACT GCGTGACTTGTGAATGTACACTGGAGGAAACGGATTCTTCAGAGAACAGCCTACATCCTGACTTTGCAAA ATACCTCACAGAAACCGAAGACACTGTCAAAACAACACGAAACATGGGCAGACTCAATCTGTTCTCCCTG GACCCAGACATAGACACCCTGAAGCTTCAAAAAAGGGACAGTTTTGAAAATGAGTTGATGATCAAACCCT TTGAAGAGAAAGCTGCCAAAAGAATCATGATCATCTGTAGAGCTCTCAACTTCAATCTTCAGGGATGTGT TACAGAGAATGAATATGACCCTGTAACCAATATTGAGCCCTTTTTTGTGAGCGTGGCACTTTACGACCTC AGAGATAACAGGAAGATCTCTGCGGACTTCCACGTGGACCTAAATCACCCTGCTGTCCGGCAGATGCTCT CGGGGACCCCACCGGCCTTGGAAAACGGCAATATTGACACCGGTACACCAAGACAATCGGAAGAACCTCA CATAAAGGGCCTTCCAGAGGAATGGTTAAAATTCCCAAAGCAGGCTGTCTTCTCTGTGAGTGATCCACAC TCTGAAATTGTTTTGGTGGCCAAAGTCGAAAAAGTCTTAATGGGAAACATCGGGAGTGGTGCTGAACCTT ATATCAAGAATCCAGACTCCAACAAGTTTGCACAAAAGATTCTAAAATCCAATAGGCAGTTCTGCAGCAA ATTGGGGAAATATCGGATGCCGTTTGCTTGGGCAGTGAGATCTGTATTTAAGGACAACCAGGGGAACGTG GACAGGGACTCACGGTTTTCACCACTGTATAGACAAGAAAGCAGCAAGATGTCGTCTGAGGACCTCCTAA AACTGGTGTCAGACTACAGAAGAGCTGACCGGATAAGCAAAATGCAGAGCATCCCTGGAAGCCTGGATAT TGCTGTGGACAACATTCCCTTGGAGCACCCAAACTGTGTAACATCGTCCTTTATCCCTGTCAAGCCCTTC AACGTGTCGGCTCAGTCAGAACCTACAGTGGAGGTGGAAGAATTTATTTATGACTCAACAAAGTATTGCC GTCCATACCGAGTATACAAAAATCAAATTTATGTATACCCCAAACACCTCAAATACGATAGCCAGAAATG CTTCAACAAGGCACGGAATATAACTGTGTGCATTGAATTCAAAAATTCAGATGACGATGGAGCCAAGCCC ATGAAGTGTATTTATGGGAAACCTGGAGGGCCCCTCTTCACTTCCTCTGCCTACACGGCTGTGCTACACC ATTCTCAAAATCCAGATTTCTCTGATGAGGTGAAAATTGAACTACCAACACAACTCCATGGAAAACATCA CCTTTTGTTCTCCTTTTACCACATCACATGTGACATCAATGCCAAAGCCAATGCCAAAAAGAAAGAGGCT TTGGAGACATCAGTGGGCTATGCATGGCTTCCTCTGATGAAACATGATCAAATAGCTTCTCAGGAGTACA ACATCCCAATAGCAACGACCCTGCCTCCTAATTATTTAAGCATTCAAGATCCTACAAGTGCAAAGCATGG TGGAAGTGACATTAAATGGGTCGATGGTGGCAAACCGCTTTTCAAAGTGTCCACATTTGTTGTATCAACA GTGAACACTCAGGACCCACATGTAAATGCATTTTTCCGTCAGTGCCAAAAAAGAGAAAAAGACATGTCTC AGTCACCTACCTCCAGCTTTGTCCGTGCCTGTAAGAACTTACTAAATGTGGACAAGATCCACTCCATCAT GAGTTTCCTGCCTATCATCTTGAACCAGCTCTTCAAAATTCTAGTGCAGAACGAGGAAGATGAAATTACT GCGACCGTCACCAGGGTTCTGGCTGACATTGTGGCCAAGTGTCATGAGGAGCAACTAGACCATTCTGTCC AGTCATACATTAAGTTTGTATTCAAGACCAAATCCTACAAAGAAAGAACAATACATGAGGAACTGGCTAA AAATTTGAGTGATCTTTTGAAGTCCAATGACTCAACGATAGTCAAGCATGTTCTAGAGCATTCTTGGTTC TTCTTTGCCATTATTCTAAAATCAATGGCACAGCACTTGATTGACACAAACAAAATTCAGCTTCCCAGAG CTCAAAGATTCCCCGAGTCTTACCAAAGCGAACTAGACAACTTGGTGATGGGCCTGTGTGACCACGTGAT TTGGAAATGCAAGGAAGCCCCTGAGGAAACCAAAAGAGCAAACCACAGCGTTGCCAGATTCCTTAAGCGC TGCTTTACATTTATGGACCGGGGATTCGTGTTTAAGATGGTGAACAATTACATCAGCATGTTCTCTTCTG GTGAGTTTAAGACTTTATGCCAGTATAAGTTTGATTTCCTTCAGGAAGTTTGTCAACATGAGCACTTTAT CCCTTTGTGTCTCCCCATAAGATCTGCAAACATTCCAGATCCCTTGACACCTTCAGAATCAATCCGAGAG TTACATGCCTCAGATATGCCTGAGTACTCAGTCACAAATGAATTTTGCCGCAAACACTTCTTAATTGGAA TTCTTCTCCGAGAAGTTGGCTTTGCCCTGCAGGAGGACCAAGACATCAGGCACTTAGCTTTAGCTGTCCT AAAAAATCTAATGGCTAAGCATTCTTTCGATGATCGATACAGGGAACCTAGGAAGCAGGCACAGATAGCG AGTCTGTACATGCCGCTCTATGGTATGCTCCTGGACAATATGCCAAGAATCTACCTGAAGGACCTGTATC CTTTCACCGTGAACACATCCAATCAGGGATCTAGAGATGACCTCAGCACTAATGGAGGATTCCAGACTCA GACGTCCATGAAACATGCAACTTCTGTGGATACATCATTTTCCAAAGATGTTTTAAATTCCATAGCAGCA TTTTCATCAATAGCTATTTCTACAGTGAACCATGCAGATTCCAGAGCGTCCTTAGCGAGCCTCGACTCCA ACCCAAGTACCACAGAGAAGAGCAGTGAGAAGACAGACAACTGTGAAAAGATCCCAAGGCCCTTGTCTTT GATTGGGTCAACGCTTCGGTTTGACAAATTAGATCAAGCAGAAACCAGGAGTCTTCTTATGTGTTTTCTT CACATTATGAAGACCATTTCAGATGAGACTCTGATTGCCTACTGGCAGAGAGCACCCAGTCCAGAGGTGT CAGACTTCTTCAGCATCTTGGACGTTTGTCTTCAGAATTTTAGATACCTAGGGAAACGCAATATAATAAG GAAAATCGCTGCAGCGTTTAAGTTTGTGCAGTCAACCCAGAACAATGGGACTCTGAAGGGATCCAATCCT TCCTGCCAGACATCAGGTCTCTTGTCACAATGGATGCACACGACTTCTGGCCACGAGGGACATAAGCAGC ACAGGTCTCAGACTTTACCTATAATCCGAGGCAAAAATGCACTTTCCAACCCCAAACTTTTACAGATGTT GGACAACAGCATGAACAGCAATTCCAATGAAATAGACATTGTCCACCATGTTGACACAGAGGCCAACATA GCCACCGAGGTCTGCCTCACTATTCTGGACCTGCTGTCTCTCTTTACCCAGGTCCACCAGAGACAGCTCC AACAATCCGACTGTCAAAATTCACTCATGAAAAGGGTCTTCGATACTTACATGCTGTTTTTCCAAGTCAA CCAGTCAGCCTCAGCCCTGAAACACGTGTTTGCTTCTTTAAGACTGTTTGTGTGCAAGTTTCCGTCAGCA TTTTTCCAAGGGCCTGCTGACCTCTGTGGCTCATTCTGCTATGAAGTCCTCAAATGCTGTAACCACAGGT CAAGGTTGACTCAGATGGAAGCTTCAGCACTTCTATACTTCTTCATGAGGAAGAACTTTGAGTTTAACAA GCAGAAGTCAATTGTCCGGTCTCACTTACAACTCATCAAAGCAGTGAGCCAGTTAATAGCTGATGCAGGG ATCGGAGGGTCTCGCTTTCAACACTCCCTTGCAATCACGAACAACTTTGCCAATGGAGATAAACAGATGA AAAACAGCAATTTCCCAGCAGAGGTGAAAGATCTGACTAAACGTATAAGGACTGTTTTGATGGCCACAGC CCAGATGAAGGAGCATGAGAAGGACCCAGAGATGCTGGTGGACCTTCAATACAGCCTAGCAAACTCCTAT GCAAGTACCCCGGAGTTACGGAGGACCTGGCTGGAAAGCATGGCCAAGATTCATGCAAGAAATGGAGACC TGTCTGAGGCTGCGATGTGTTACATCCATATAGCTGCACTTATTGCAGAATACCTGAAGCGCAAGGGTTA CTGGAAAATGGAAAAGATTTGCACACCACCCCTGCTTCCAGAAGACACCCAACCCTGTGATAGCAACTTA TTACTAACAACTCCAGGCGGAGGAAGCATGTTCTCTATGGGATGGCCAGCCTTTCTGAGCATCACCCCAA ACATTAAAGAAGAAGGAGCAATGAAAGAGGATTCTGGAATGCAAGACACCCCGTACAATGAGAACATCCT AGTGGAACAGCTGTATATGTGTGTGGAGTTCCTTTGGAAGTCTGAACGATACGAACTCATCGCTGATGTC AATAAGCCCATCATCGCTGTCTTTGAAAAGCAACGAGACTTCAAAAAATTATCAGATCTCTATTATGACA TCCACCGGTCCTATCTGAAAGTGGCAGAGGTGGTGAATTCGGAGAAGCGATTGTTTGGTCGTTACTATAG AGTGGCGTTTTATGGGCAGGGATTCTTTGAGGAGGAGGAAGGTAAAGAGTATATCTACAAAGAGCCTAAG CTGACAGGGCTCTCGGAGATCTCCCAAAGGCTTCTCAAGCTCTATGCAGACAAATTTGGAGCAGACAATG TGAAAATAATTCAAGATTCCAACAAGGTAAACCCCAAGGATCTGGACCCCAAATATGCCTATATTCAGGT GACCTATGTCACACCATTCTTTGAAGAAAAGGAAATCGAGGACCGAAAGACAGACTTTGAAATGCATCAC AACATCAATCGCTTTGTCTTTGAGACACCCTTCACTCTGTCAGGCAAGAAGCACGGAGGAGTGGCTGAGC AGTGCAAGCGGAGGACAGTCCTGACCACAAGCCACTTGTTCCCCTACGTAAAGAAGAGGATCCAGGTCAT CAGCCAATCAAGCACAGAGCTGAATCCTATCGAGGTGGCAATTGATGAGATGTCCAGGAAGGTCTCTGAG CTTAATCAGCTGTGCACCACAGAGGAGGTGGATATGATCCGCCTACAGCTCAAACTCCAGGGCAGTGTCA GCGTGAAGGTCAATGCTGGGCCAATGGCTTATGCTCGAGCCTTTCTTGAAGAAACTAATGCAAAGAAGTA TCCTGACAACCAAGTTAAGCTACTAAAGGAAATATTCAGGCAATTTGCAGATGCGTGTGGGCAGGCTCTT GATGTGAATGAGCGTCTCATCAAGGAAGACCAGCTGGAGTACCAGGAAGAACTGAGGTCCCATTATAAGG ACATGCTCAGTGAACTGTCTGCCATCATGAATGAGCAGATTACGGGCAGGGACGACCCAGCAAAGTGCGG AGTGGAGCGACCCTACACCACACGTGTAACTAGCAAGGGGACCGCGGCTGTACCTGTGGTCTCCATCTCA TCCAGTGCGGAGGTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285927
Insert Size	6528 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285927.1, NP_001272856.1
RefSeq Size	7324 bp
RefSeq ORF	6528 bp
Locus ID	210293
UniProt ID	Q8BZN6
Gene Summary	Guanine nucleotide-exchange factor (GEF) that activates CDC42 and RAC1 by exchanging bound GDP for free GTP. Essential for dendritic spine morphogenesis in Purkinje cells and in hippocampal neurons, via a CDC42-mediated pathway (PubMed:25851601). Sustains B-cell lymphopoiesis in secondary lymphoid tissues and regulates FCER2/CD23 expression (PubMed:27502165).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2, alternatively referred to as DOCK10.2) contains an alternate first exon and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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