Myo7a (NM_001256081) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229722 representing NM_001256081 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTATTCTGCAGAAGGGGGACTATGTATGGATGGACCTGAAGTCAGGCCAGGAGTTTGATGTGCCCA TCGGGGCCGTGGTGAAGCTCTGCGACTCGGGCCAGATCCAGGTGGTGGATGATGAAGACAATGAACACTG GATATCCCCTCAGAATGCCACGCACATCAAGCCAATGCACCCCACATCGGTGCACGGCGTGGAGGACATG ATCCGCCTGGGGGATCTCAACGAGGCAGGCATCCTTCGAAACCTTCTCATTCGCTACCGGGACCACCTCA TCTATACGTACACAGGTTCCATCCTGGTGGCCGTGAACCCCTACCAGCTGCTCTCCATCTACTCGCCAGA GCACATCCGCCAGTACACCAACAAGAAGATAGGGGAGATGCCCCCCCACATCTTCGCCATTGCTGACAAC TGCTACTTCAACATGAAACGCAACAACCGGGACCAGTGCTGTATTATCAGCGGGGAGTCGGGAGCTGGCA AGACAGAGAGCACAAAGTTGATCCTGCAGTTCCTGGCAGCCATCAGTGGACAGCACTCATGGATCGAGCA GCAGGTGCTGGAGGCCACCCCGATCCTGGAAGCATTTGGGAACGCCAAGACCATCCGCAACGACAACTCT AGCCGCTTTGGCAAGTACATTGACATCCACTTTAACAAGCGTGGTGCCATCGAGGGCGCCAAAATAGAGC AATACCTGCTGGAGAAGTCACGTGTCTGCCGCCAGGCCCCTGACGAGAGGAACTATCACGTGTTCTACTG TATGCTGGAGGGCATGAATGAGGAGGAGAAGAAGAAACTGGGCCTAGGCCAGGCCGCTGACTACAACTAC TTGGCCATGGGTAACTGCATCACCTGTGAGGGCCGCGTGGACAGTCAGGAGTATGCCAACATCCGCTCTG CCATGAAGGTTCTCATGTTCACAGACACGGAGAACTGGGAGATCTCGAAGCTTCTGGCTGCCATCCTACA CATGGGCAATCTGCAGTATGAGGCCCGGACATTTGAGAACTTGGATGCGTGTGAAGTCCTCTTCTCCCCA TCGCTGGCCACGGCAGCTTCTCTGCTCGAGGTGAACCCCCCAGACCTGATGAGCTGCCTCACCAGCCGCA CCCTCATCACCCGTGGGGAGACGGTGTCCACCCCTCTCAGCAGGGAACAGGCGCTGGATGTGCGAGATGC CTTTGTCAAGGGCATCTATGGGCGGCTCTTTGTGTGGATTGTGGAGAAGATCAACGCAGCAATCTACAAG CCACCCCCCCTGGAAGTGAAGAACTCTCGCCGGTCCATCGGTCTCCTGGACATCTTTGGATTTGAGAACT TCACTGTGAACAGCTTCGAGCAGCTCTGCATTAACTTTGCCAATGAGCACCTGCAGCAATTCTTCGTGCG GCACGTGTTCAAGCTGGAGCAGGAGGAGTACGACCTGGAGAGCATCGACTGGTTGCACATTGAGTTCACT GACAACCAGGAAGCACTGGACATGATTGCCAACCGGCCTATGAACGTCATCTCCCTCATCGATGAGGAGA GCAAGTTCCCCAAGGGCACGGATGCCACCATGCTGCATAAGCTGAACTCACAGCACAAGCTCAATGCCAA CTACGTGCCACCCAAGAACAGCCACGAGACCCAGTTTGGAATCAACCACTTTGCGGGTGTTGTCTATTAT GAGAGTCAAGGCTTCCTGGAGAAGAACCGAGACACCCTGCATGGGGACATCATCCAGCTGGTCCACTCTT CCCGGAACAAGTTCATAAAGCAGATTTTCCAAGCTGACGTTGCCATGGGTGCCGAGACCAGGAAGCGCTC GCCTACACTCAGCAGCCAGTTCAAGCGGTCTCTGGAGCTGCTGATGCGCACACTGGGCGCCTGCCAGCCC TTCTTTGTGCGTTGTATCAAACCCAATGAGTTCAAGAAGCCCATGCTCTTCGACCGGCACTTGTGTGTAC GCCAGCTGCGATATTCGGGCATGATGGAGACAATCCGCATCCGCCACGCAGGCTACCCCATTCGCTACAG CTTTGTGGAGTTTGTGGAGCGCTACCGGGTACTGCTGCCTGGTGTGAAGCCAGCATACAAGCAGGGTGAC CTCCGAGGGACATGCCAGCGCATGGCTGAGGCTGTGCTGGGCACGCACGATGACTGGCAGATTGGCAAAA CCAAGATCTTTCTGAAGGACCACCATGACATGTTGCTGGAGGTGGAGCGGGACAAGGCCATCACAGACAG AGTCATTCTCCTCCAGAAGGTTATCCGGGGCTTCAAAGACAGGTCCAACTTCCTGAGACTGAAGAGTGCT GCCACACTGATCCAGAGGCACTGGCGGGGCCACCACTGTAGGAAAAACTATGAGCTGATTCGTCTTGGCT TCCTGCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCTGCACAAGCAGTACCGCCTGGCCAGACAGCGCAT AATAGAGTTCCAGGCCCGCTGCCGGGCCTATCTGGTGCGCAAGGCCTTCCGCCACCGCCTCTGGGCCGTG ATCACCGTGCAGGCCTATGCCCGAGGCATGATTGCCCGCCGGCTACACCGCCGCCTCCGGGTTGAGTACC AGCGGCGCCTCGAGGCAGAGAGGATGCGTCTGGCAGAGGAGGAGAAACTCCGAAAGGAGATGAGTGCCAA GAAGGCCAAAGAGGAGGCTGAGCGCAAGCATCAGGAGCGCCTGGCTCAGCTAGCCCGCGAGGATGCGGAG CGGGAACTGAAGGAGAAGGAGGAGGCTCGGAGGAAGAAGGAACTGCTGGAGCAGATGGAGAAGGCCCGCC ACGAACCCATCAACCACTCAGATATGGTGGACAAGATGTTTGGCTTCCTGGGGACTTCAGGCAGCCTGCC AGGCCAGGAAGGCCAGGCGCCTAGTGGCTTTGAGGACCTAGAGCGCGGACGGAGGGAGATGGTGGAAGAG GATGTTGACGCTGCCCTGCCCCTGCCTGATGAAGACGAGGAGGACCTTTCTGAGTACAAATTCGCCAAGT TTGCTGCCACCTACTTCCAGGGCACAACCACACACTCCTACACCCGGAGGCCTCTCAAGCAGCCGCTGCT CTACCACGACGATGAGGGTGACCAGCTGGCGGCGCTGGCTGTCTGGATCACCATCCTCCGGTTCATGGGG GACCTCCCAGAGCCCAAGTACCACACAGCCATGAGCGACGGCAGTGAGAAGATCCCAGTGATGACTAAGA TCTACGAGACCCTAGGCAAGAAGACATATAAGAGGGAGCTGCAGGCCTTGCAGGGCGAGGGCGAGACCCA GCTCCCTGAGGGGCAGAAGAAGACCAGTGTGAGACACAAGTTGGTACACTTGACACTGAAGAAAAAGTCC AAACTCACAGAAGAGGTGACCAAGAGGCTGAACGATGGGGAATCCACGGTACAGGGCAACAGCATGCTGG AGGATCGGCCCACCTCAAATCTAGAGAAGCTGCACTTCATCATCGGCAACGGCATCCTGCGGCCTGCGCT GCGGGACGAGATTTACTGCCAGATCAGTAAGCAGCTCACACACAACCCATCCAAGAGCAGCTATGCCAGG GGCTGGATCCTCGTGTCGCTCTGTGTGGGCTGCTTCGCCCCCTCTGAGAAGTTCGTTAAGTACCTGCGGA ACTTCATCCACGGAGGCCCACCTGGCTATGCTCCTTACTGTGAGGAGCGCCTGAGGAGGACCTTTGTCAA CGGAACTCGGACACAGCCACCCAGCTGGCTGGAGCTGCAGGCCACCAAGTCCAAGAAGCCCATCATGTTG CCCGTGACCTTCATGGATGGGACCACCAAGACCCTGCTAACAGATTCAGCAACTACAGCCAGGGAGCTGT GCAATGCTCTGGCTGACAAGATCTCACTCAAGGACCGCTTTGGCTTCTCCCTCTACATCGCTCTGTTCGA TAAGGTGTCCTCCCTGGGCAGCGGCAGTGACCATGTCATGGATGCCATCTCTCAGTGTGAGCAGTACGCC AAGGAGCAGGGTGCTCAGGAGCGCAACGCCCCATGGAGGCTCTTCTTTAGAAAGGAGGTCTTCACACCCT GGCACAACCCCTCGGAGGACAACGTGGCCACGAACCTCATCTACCAGCAGGTGGTGCGAGGAGTCAAGTT TGGGGAGTACAGGTGTGAGAAGGAGGACGACCTGGCTGAGCTGGCTTCTCAGCAGTACTTTGTGGACTAT GGTTCTGAGATGATTCTGGAGCGCCTGCTGAGCCTCGTGCCCACTTACATCCCTGACCGTGAGATCACAC CGCTGAAGAATCTTGAGAAGTGGGCACAGCTGGCCATTGCTGCCCACAAGAAGGGAATTTATGCCCAGAG GAGAACTGACTCCCAGAAGGTCAAAGAGGATGTGGTCAATTATGCCCGTTTCAAGTGGCCCTTGCTCTTC TCCAGGTTTTACGAAGCTTACAAATTCTCAGGCCCTCCCCTCCCCAAGAGCGACGTCATCGTGGCTGTCA ACTGGACGGGTGTGTACTTCGTGGACGAGCAGGAGCAGGTGCTTCTGGAGCTGTCCTTCCCGGAGATCAT GGCTGTGTCCAGCAGTAGGGAGTGCCGCGTCTTGCTCTCACTGGGCTGCTCTGACTTGGGCTGTGCTACT TGTCAATCGGGCCGGGCAGGGCTGACCCCGGCTGGACCCTGTTCTCCGTGTTGGTCCTGTAGGGGAACAA AGATGATGGCCCCCAGCTTTACCCTGGCCACCATCAAAGGAGATGAGTACACCTTCACATCCAGCAATGC TGAGGACATCCGTGACCTGGTGGTCACCTTTCTGGAGGGGCTACGGAAGAGGTCTAAGTATGTGGTGGCA CTGCAGGACAATCCTAACCCTGCTGGTGAGGAGTCAGGCTTCCTCAGCTTCGCCAAGGGAGACCTCATCA TCCTTGACCATGATACTGGTGAGCAGGTCATGAACTCAGGCTGGGCCAACGGCATCAACGAGAGGACCAA GCAGCGCGGCGACTTCCCCACTGACTGTGTATACGTCATGCCCACTGTCACCTTGCCACCAAGGGAGATT GTGGCCCTGGTCACTATGACCCCAGACCAGAGGCAGGATGTCGTCCGGCTCCTGCAGCTTCGCACAGCAG AGCCAGAGGTGCGCGCCAAGCCCTACACGCTAGAGGAGTTCTCCTACGACTACTTCAGGCCCCCACCCAA GCACACGCTGAGCCGTGTCATGGTGTCCAAGGCCCGCGGTAAGGACAGGCTGTGGAGCCACACACGAGAG CCCCTCAAGCAGGCCCTGCTCAAGAAGATCCTGGGCAGTGAAGAACTCTCCCAGGAAGCCTGCATGGCCT TTGTAGCTGTGCTCAAGTACATGGGCGACTACCCATCCAAGAGGATGCGATCCGTCAATGAGCTCACTGA CCAGATCTTTGAGTGGGCACTCAAGGCTGAGCCCCTCAAGGATGAGGCCTACGTGCAGATCCTGAAGCAG CTGACTGACAATCACATCAGGTACAGCGAAGAGAGGGGCTGGGAACTGCTGTGGCTGTGCACGGGCCTCT TCCCGCCCAGCAACATCCTCCTGCCTCATGTTCAGCGGTTTCTGCAGTCCCGCAAGCACTGTCCTCTTGC CATTGACTGCCTGCAGAGGCTCCAGAAAGCCCTGAGAAATGGCTCCCGGAAGTACCCTCCGCACCTGGTG GAGGTGGAGGCCATCCAACATAAGACTACCCAGATCTTCCACAAGGTCTACTTCCCCGATGACACGGACG AGGCTTTTGAGGTGGAGTCCAGCACCAAGGCCAAGGACTTCTGCCAGAACATCGCCAGCCGGCTGCTGCT CAAGTCTTCCGAGGGATTCAGCCTTTTTGTCAAAATCGCAGATAAGGTCATCAGCGTCCCAGAGAATGAT TTCTTCTTTGACTTTGTCCGACACCTGACAGACTGGATAAAGAAAGCACGGCCCATCAAGGACGGAATCG TGCCCTCACTAACCTACCAGGTGTTCTTCATGAAGAAGCTGTGGACCACCACAGTGCCGGGCAAGGACCC CATGGCTGACTCCATCTTCCACTATTACCAGGAACTGCCCAAATATCTCCGAGGCTACCACAAGTGCACC CGGGAGGAGGTGCTGCAGCTGGGCGCACTCATCTACAGGGTCAAGTTTGAGGAGGACAAATCCTACTTCC CTAGCATCCCCAAGTTGCTGAGGGAGCTGGTACCCCAGGACCTAATCCGGCAGGTCTCACCTGATGACTG GAAACGGTCTATTGTCGCCTACTTCAACAAACATGCGGGGAAGTCCAAGGAGGAAGCCAAGCTGGCCTTC CTCAAACTCATCTTCAAGTGGCCCACCTTTGGCTCAGCCTTCTTTGAGGTGAAGCAAACTACAGAACCAA ACTTCCCAGAGATTCTCTTAATTGCCATCAACAAGTACGGGGTCAGCCTCATCGATCCCAGAACCAAGGA CATCCTGACTACTCACCCCTTCACCAAGATCTCCAACTGGAGTAGTGGCAACACCTACTTCCACATCACC ATTGGGAACTTGGTCCGTGGGAGCAAACTGCTCTGTGAGACATCGCTGGGATACAAAATGGATGATCTTC TGACTTCCTACATCAGCCAGATGCTCACAGCCATGAGCAAGCAGAGGAACTCCAGGAGTGGAAGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256081
Insert Size	6648 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256081.1, NP_001243010.1
RefSeq Size	7481 bp
RefSeq ORF	6648 bp
Locus ID	17921
UniProt ID	P97479
Gene Summary	Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. In the retina, plays an important role in the renewal of the outer photoreceptor disks. Plays an important role in the distribution and migration of retinal pigment epithelial (RPE) melanosomes and phagosomes, and in the regulation of opsin transport in retinal photoreceptors. Mediates intracellular transport of RPE65 in the retina pigment epithelium. In the inner ear, plays an important role in differentiation, morphogenesis and organization of cochlear hair cell bundles. Motor protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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