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Sat1 (NM_009121) Mouse Tagged ORF Clone
CAT＃: MG201444
- TrueORF®
Sat1 (tGFP-tagged) - Mouse spermidine/spermine N1-acetyl transferase 1 (Sat1)
ORF Plasmid: DDK
"NM_009121" in other vectors (6)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA617398; Sat; SSAT |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG201444 representing NM_009121
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAAATTTAAGATCCGTCCAGCCACTGCCTCTGACTGCAGTGACATCCTGCGACTGATCAAGGAAC TGGCTAAATATGAATACATGGAAGATCAAGTCATTTTAACTGAGAAAGATCTCCAAGAGGATGGCTTTGG AGAACACCCCTTCTACCACTGCCTGGTTGCAGAAGTGCCTAAAGAGCACTGGACCCCTGAAGGACATAGC ATTGTTGGGTTCGCCATGTACTATTTTACCTATGACCCATGGATTGGCAAGTTGCTGTATCTTGAAGACT TCTTCGTGATGAGTGATTACAGAGGCTTTGGTATAGGATCAGAAATTTTGAAGAATCTAAGCCAGGTTGC CATGAAGTGTCGCTGCAGCAGTATGCACTTCTTGGTAGCAGAATGGAATGAACCATCTATCAACTTCTAC AAAAGAAGAGGTGCTTCGGATCTGTCCAGTGAAGAGGGATGGAGGCTCTTCAAGATTGACAAAGAGTACT TGCTAAAAATGGCAGCAGAGGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201444 representing NM_009121
Red=Cloning site Green=Tags(s) MAKFKIRPATASDCSDILRLIKELAKYEYMEDQVILTEKDLQEDGFGEHPFYHCLVAEVPKEHWTPEGHS IVGFAMYYFTYDPWIGKLLYLEDFFVMSDYRGFGIGSEILKNLSQVAMKCRCSSMHFLVAEWNEPSINFY KRRGASDLSSEEGWRLFKIDKEYLLKMAAEE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009121 |
| ORF Size | 513 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009121.4 |
| RefSeq Size | 1233 bp |
| RefSeq ORF | 516 bp |
| Locus ID | 20229 |
| UniProt ID | P48026 |
| Gene Summary | Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells. Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N(1)- and N(8)-acetylspermidine), spermine, N(1)-acetylspermidine and N(8)-acetylspermidine (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202652 | Sat1 (untagged) - Mouse spermidine/spermine N1-acetyl transferase 1 (Sat1), (10ug) |
CNY 2,000.00 |
|
| MR201444 | Sat1 (Myc-DDK-tagged) - Mouse spermidine/spermine N1-acetyl transferase 1 (Sat1) |
CNY 3,600.00 |
|
| MR201444L1 | Lenti ORF clone of Sat1 (Myc-DDK-tagged) - Mouse spermidine/spermine N1-acetyl transferase 1 (Sat1) |
CNY 6,000.00 |
|
| MR201444L2 | Lenti ORF clone of Sat1 (mGFP-tagged) - Mouse spermidine/spermine N1-acetyl transferase 1 (Sat1) |
CNY 6,000.00 |
|
| MR201444L3 | Lenti ORF clone of Sat1 (Myc-DDK-tagged) - Mouse spermidine/spermine N1-acetyl transferase 1 (Sat1) |
CNY 5,890.00 |
|
| MR201444L4 | Lenti ORF clone of Sat1 (mGFP-tagged) - Mouse spermidine/spermine N1-acetyl transferase 1 (Sat1) |
CNY 5,890.00 |
