Rheb (BC012273) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Rheb1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG201682 representing BC012273 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCAGTCCAAGTCCCGGAAGATCGCCATCCTGGGCTACCGGTCTGTGGGAAAGTCCTCATTGACAA TTCAGTTTGTTGAAGGCCAATTTGTTGATTCCTACGATCCAACCATAGAGAACACGTTCACCAAGTTGAT CACGGTAAATGGTCAAGAGTATCATCTTCAGCTTGTAGACACAGCGGGGCAGGATGAATATTCCATTTTT CCTCAGACATACTCCATAGATATTAATGGTTATATTCTTGTGTATTCTGTTACATCAATCAAAAGTTTTG AAGTAATTAAAGTTATCCATGGCAAGTTGTTGGATATGGTGGGGAAAGTGCAGATACCTATTATGTTGGT TGGAAATAAGAAGGACCTGCATATGGAAAGGGTGATCAGCTATGAAGAAGGAAAGGCTTTGGCAGAATCT TGGAATGCAGCTTTTTTGGAATCTTCTGCTAAAGAAAATCAAACTGCTGTTGATGTTTTTAAAAGGATAA TTTTGGAAGCAGAAAAGATTGATGGAGCAGCTTCACAAGGAAAGTCTTCGTGCTCGGTGATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG201682 representing BC012273 Red=Cloning site Green=Tags(s) MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIF PQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAES WNAAFLESSAKENQTAVDVFKRIILEAEKIDGAASQGKSSCSVM TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC012273
ORF Size	554 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC012273, AAH12273
RefSeq Size	1178 bp
RefSeq ORF	554 bp
Locus ID	19744
Gene Summary	Activates the protein kinase activity of mTORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Has low intrinsic GTPase activity.[UniProtKB/Swiss-Prot Function]

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