Mocs2 (BC024371) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AI415403
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG201790 representing BC024371 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAGCTTGGAGATCAGCAACTCCTGCTTCAGCCCGGAGACGAGGTCGCCATCATCCCGCCAATCAG TGGAGGATAATGCATCTGAGCCTTCTGGGAAAGATGTGGACGATGTCCAGGAGAAACCTAAAGACATAAT ACAGTTCACTGCCGAGAAGCTCTCTGTGGGGGAAGTGTCACAGTTGGTGGTGTCCCCTCTGTGTGGTGCA GTGTCTCTCTTTGTAGGGACTACAAGAAATAACTTTGAAGGCAAGAAAGTCATTAGCTTAGAATATGAAG CGTATGTACCGATGGCAGAAAATGAAATCAGAAAAATTTGTAATGACATTAGACAGAAATGGCCCGTGAG ACACATAGCAGTATTCCATCGGCTTGGTTTGGTTCCAGTGTCAGAAGCAAGCACAGTTATTGCTGTGTCT TCAGCTCACAGAGCCGCGTCCCTCGAAGCCGTGAGCTACGCCATTGATTCTTTAAAAGCCAAGGTGCCCA TATGGAAAAAGGAAATATATGAAGAATCAACCTCATCTTGGAAAAGAAACAAAGAGTGCTTCTGGGCAGC TGGTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG201790 representing BC024371 Red=Cloning site Green=Tags(s) MSSLEISNSCFSPETRSPSSRQSVEDNASEPSGKDVDDVQEKPKDIIQFTAEKLSVGEVSQLVVSPLCGA VSLFVGTTRNNFEGKKVISLEYEAYVPMAENEIRKICNDIRQKWPVRHIAVFHRLGLVPVSEASTVIAVS SAHRAASLEAVSYAIDSLKAKVPIWKKEIYEESTSSWKRNKECFWAAGD TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC024371
ORF Size	567 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC024371, AAH24371
RefSeq Size	1177 bp
RefSeq ORF	569 bp
Locus ID	17434
Gene Summary	Eukaryotic molybdoenzymes use a unique molybdenum cofactor (MoCo) consisting of a pterin, termed molybdopterin, and the catalytically active metal molybdenum. MoCo is synthesized from precursor Z by the heterodimeric enzyme molybdopterin synthase. The large and small subunits of molybdopterin synthase are both encoded from this gene by overlapping open reading frames. The proteins were initially thought to be encoded from a bicistronic transcript. Based on experiments with the human molybdopterin synthase ortholog, they are now thought to be encoded from monocistronic transcripts. Alternatively spliced transcripts have been found for this locus that encode the large and small subunits. [provided by RefSeq, Jul 2008]

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