Tcte3 (BC061136) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	D17Leh117c; LC2; Tcte-3; Tctex-2; Tctex-4; Tctex2; Tctex4
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG201829 representing BC061136 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGCGAGGCCGAATGGCGAAGACGCCCACCGGCCAAACGCATCAATCCCCGGTGTCTAAGAGAG AAAGGAAGCCTAGCATGTTCGAGAAGGAGTCATATGCACAGATCTTAAGAGAAAGACTGAGAGAGTCTTT TCATGATGTTCAGTACGTGGAACCTCCGTTTGATGACTCAATTGCTGATGTAGGCAAAGAATGGAAAAGT GCCCTGGCAAAATTAAAGTTTGCTAATTCATACAGAATGGAGCCACTGAAGAAATTTCAAGCACATTTGG TAGAAACTAAAATCCAGCAGATATTAAAGGACAGTCTTAAAGATGTCAAATATGATGACAAAGCCCCTCA TTTGTCACTTGAATTGGCAGATCGAATATTGGCAGCAGTCAAAGAATTTGCATACCATCGTTATAAATTC ATTATACAAGTATTATTTATTCAAAAGACTGGTCAAGCAATAAATATTGCCAGCAGATGGATCTGGGATG TGGCATGGGACAACTGGGTAGAAGCTAAACATGAAACAGAGTCTTACGTGGTATTGGCCTTGGTGTTTGC TCTCTATTGTGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG201829 representing BC061136 Red=Cloning site Green=Tags(s) MERRGRMAKTPTGQTHQSPVSKRERKPSMFEKESYAQILRERLRESFHDVQYVEPPFDDSIADVGKEWKS ALAKLKFANSYRMEPLKKFQAHLVETKIQQILKDSLKDVKYDDKAPHLSLELADRILAAVKEFAYHRYKF IIQVLFIQKTGQAINIASRWIWDVAWDNWVEAKHETESYVVLALVFALYCE TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC061136
ORF Size	573 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC061136, AAH61136
RefSeq Size	721 bp
RefSeq ORF	575 bp
Locus ID	21647
Gene Summary	This gene is one of three genes with a very high degree of similarity to each other within a 77 kb genomic span on Chromosome 17 A2. This gene is the most distal copy of the three genes. [provided by RefSeq, Jul 2008]

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