Ccdc68 (BC046343) Mouse Tagged ORF Clone
CAT＃: MG203001
- TrueORF®
Ccdc68 (tGFP-tagged) - Mouse coiled-coil domain containing 68 (cDNA clone MGC:54896 IMAGE:6487853)
ORF Plasmid: DDK
"BC046343" in other vectors (4)
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CNY 2660.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MGC54896 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203001 representing BC046343
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAACTGTAACAGTGACCACAGAAGTTCCCTCAAGCGGTAAGACAGAAGACGGCCACGTCTTCTGTG ACTCTTCGTCTGCTCACATTATTGAAGAAACCGAGTATGTGAGACAGATGCGAACTACACTGGAAAAGAT CCGAAATCACATGTTTAAGGAAAAAGAAGGCTGTGGCAATGCAAGGCACAAACTAGATGCAGAGGGATCT GGAAATATTCAGAATGGCTCAGACTCCACCACAGACCCCACCTGTCTGGATTTGCTCATGGAAAACATGA GAAGAAAAGATCAGCAGCTCCTGGAAATGAACAGAGAGAATGAAGTGTTGCAAATCAAGCTGGAAGCTTC CAGAGAAGCAGGAGCAGCAGCTTTGAGAAATGTGGCTCAGAGATTATTTGACAACTACCAAACACAAGCT GGAGACCTGGAGAAAAAGCACGAGGGCAGGAAACATCTCCTTCAGGTCAACAACCTTGAGAAAGAGCAGG CATTAAAGGGAAGCGCCGAAAGTCTGAATCTACTTTCAGAAAAACTGGAGGAAAAACATGGGCAAATCGT AGGGCTGGAAAACCGTGTACAGAGGATGGAAAACAATAAAGAACTCAAAACCAGGGTGGCACATTGGACT GAAACACCTAGGACCTTGGTATCTAAGGCCGTCTCCACAAGCGAGTTGAAGACTGAAGGTGCTTCTCCAT ATTTGATGTTAATTCGGCTACGAAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203001 representing BC046343
Red=Cloning site Green=Tags(s) MTTVTVTTEVPSSGKTEDGHVFCDSSSAHIIEETEYVRQMRTTLEKIRNHMFKEKEGCGNARHKLDAEGS GNIQNGSDSTTDPTCLDLLMENMRRKDQQLLEMNRENEVLQIKLEASREAGAAALRNVAQRLFDNYQTQA GDLEKKHEGRKHLLQVNNLEKEQALKGSAESLNLLSEKLEEKHGQIVGLENRVQRMENNKELKTRVAHWT ETPRTLVSKAVSTSELKTEGASPYLMLIRLRK TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC046343 |
| ORF Size | 726 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC046343, AAH46343 |
| RefSeq Size | 1247 bp |
| RefSeq ORF | 728 bp |
| Locus ID | 381175 |
| Gene Summary | Centriolar protein required for centriole subdistal appendage assembly and microtubule anchoring in interphase cells (PubMed:28422092). Together with CCDC120, cooperate with subdistal appendage components ODF2, NIN and CEP170 for hierarchical subdistal appendage assembly (PubMed:28422092).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218123 | Ccdc68 (untagged) - Mouse coiled-coil domain containing 68 (cDNA clone MGC:54896 IMAGE:6487853), (10ug) |
CNY 6940.00 |
|
| MR203001 | Ccdc68 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 68 (cDNA clone MGC:54896 IMAGE:6487853) |
CNY 2380.00 |
|
| MR203001L3 | Lenti ORF clone of Ccdc68 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 68 (cDNA clone MGC:54896 IMAGE:6487853) |
CNY 4280.00 |
|
| MR203001L4 | Lenti ORF clone of Ccdc68 (mGFP-tagged) - Mouse coiled-coil domain containing 68 (cDNA clone MGC:54896 IMAGE:6487853) |
CNY 4280.00 |
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