Cdca3 (NM_013538) Mouse Tagged ORF Clone
CAT＃: MG203531
- TrueORF®
Cdca3 (tGFP-tagged) - Mouse cell division cycle associated 3 (Cdca3)
ORF Plasmid: DDK
"NM_013538" in other vectors (4)
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CNY 2850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2410005A12Rik; C8; Grcc8; Tome-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203531 representing NM_013538
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTTCGACTCAGAGCGTCTCAGGCACTCCAGCACGGCCTCTGCCACGCAACAAGCAAGTGGCTCGAG TAGCAGACCCTCGTTCACCTAGTGCTGGCATCCAGCGCACTCCTATTCAGGTAGAGAGCTCTCCACAGCC AAGCCTACCAGCAGAACAGCTGAACGGTCTCAAACAGGCACAAGACCCAGATCCCCGCTCTCCTACTCTT GGCATTGCACGGACACCCATGAAGATCAGTGGTCCAGACCCTCAGTGCTCACTGGTGAAAGAGCTGAGCG AAGTATTGGAGACGGAAGCGTCGGAATCGATTTCCTCCCCAGAGCTTGCTCTGCCCCGGGAAACGCCTTT ATTTTCTGACCTGGACCTGTCTTCAGGTCCTCAGTTATCCCCTGAGGACCAGTTACTGCCTTGGAGCCAG GCTGAACTCGATCCCAAACAGGTGTTTACCAAGGAGGAAGCCAAACAATCCGCAGAAACTATAGCTGCCA GCCAGAACTCAGACAAGCCCTCCAGAGACCCAGAGACTCCCCAGTCCTCAGGTTCTAAGCGCAGCAGACG AAAAGCAAACAGCAAGGTTCTAGGGAGGTCCCCTCTCACCATCCTGCAGGATGACAACTCCCCTGGGACC TTGACACTACGACAGGGTAAGCGGCCTTCTGCCCTCAGTGAGAACGTTAAGGACCTAAAGGAAGGAGTCG TTCTTGGAACTGGAAGATTTCTCAAAGCTGGAGGAGGAGCACGGGAGCCAAACCAGGACCACGACAAGGA AAATCAGCATTTTGCCTTGTTGGAGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203531 representing NM_013538
Red=Cloning site Green=Tags(s) MGSTQSVSGTPARPLPRNKQVARVADPRSPSAGIQRTPIQVESSPQPSLPAEQLNGLKQAQDPDPRSPTL GIARTPMKISGPDPQCSLVKELSEVLETEASESISSPELALPRETPLFSDLDLSSGPQLSPEDQLLPWSQ AELDPKQVFTKEEAKQSAETIAASQNSDKPSRDPETPQSSGSKRSRRKANSKVLGRSPLTILQDDNSPGT LTLRQGKRPSALSENVKDLKEGVVLGTGRFLKAGGGAREPNQDHDKENQHFALLES TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_013538 |
| ORF Size | 798 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013538.3, NP_038566.1 |
| RefSeq Size | 1426 bp |
| RefSeq ORF | 801 bp |
| Locus ID | 14793 |
| UniProt ID | Q99M54 |
| Gene Summary | F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200366 | Cdca3 (untagged) - Mouse cell division cycle associated 3 (Cdca3), (10ug) |
CNY 2400.00 |
|
| MR203531 | Cdca3 (Myc-DDK-tagged) - Mouse cell division cycle associated 3 (Cdca3) |
CNY 2400.00 |
|
| MR203531L3 | Lenti ORF clone of Cdca3 (Myc-DDK-tagged) - Mouse cell division cycle associated 3 (Cdca3) |
CNY 4750.00 |
|
| MR203531L4 | Lenti ORF clone of Cdca3 (mGFP-tagged) - Mouse cell division cycle associated 3 (Cdca3) |
CNY 4750.00 |
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