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Sfrp1 (NM_013834) Mouse Tagged ORF Clone
CAT＃: MG204470
- TrueORF®
Sfrp1 (tGFP-tagged) - Mouse secreted frizzled-related sequence protein 1 (Sfrp1)
ORF Plasmid: DDK
"NM_013834" in other vectors (4)
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CNY 5,200.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2210415K03Rik; AW011917; AW107218; AW742929; sFRP-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204470 representing NM_013834
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGTCGGGCGCAGCGCGCGGGGTCGCGGCGGGGCCGCCTCGGGAGTGCTGCTGGCGTTGGCCGCCG CTCTGCTGGCCTCGGGTTCGGCCAGCGAGTACGACTACGTGAGCTTCCAGTCCGACATCGGCTCGTATCA GAGCGGGCGCTTCTACACCAAGCCCCCGCAGTGCGTGGACATCCCGGTGGACCTGAGGCTGTGCCACAAC GTGGGCTACAAGAAGATGGTGCTGCCCAACCTGCTGGAGCACGAGACCATGGCAGAGGTGAAGCAGCAGG CCAGCAGCTGGGTGCCGCTGCTCAACAAGAACTGCCACATGGGCACCCAGGTCTTCCTCTGTTCGCTCTT CGCGCCCGTCTGTCTGGACCGGCCCATCTACCCGTGTCGCTGGCTCTGCGAGGCCGTGCGCGACTCGTGC GAGCCGGTCATGCAGTTCTTCGGCTTCTACTGGCCCGAGATGCTCAAATGTGACAAGTTCCCCGAGGGCG ACGTCTGCATCGCCATGACCCCGCCCAATACCACGGAAGCCTCTAAGCCCCAAGGTACAACCGTGTGTCC TCCATGCGACAACGAGTTGAAGTCAGAGGCCATCATTGAACATCTCTGTGCAAGCGAGTTTGCACTGAGG ATGAAAATCAAAGAAGTGAAGAAGGAAAACGGTGACAAGAAGATTGTCCCCAAGAAGAAGAAACCCTTGA AGCTGGGGCCCATCAAGAAGAAGGAGCTGAAGCGGCTTGTGCTGTTCCTGAAGAACGGTGCCGACTGTCC CTGCCACCAGCTGGACAACCTCAGCCACAACTTTCTCATCATGGGCCGCAAGGTGAAGAGCCAGTACCTG CTGACAGCCATTCACAAGTGGGACAAGAAAAACAAGGAGTTCAAAAACTTCATGAAGAGAATGAAAAACC ACGAGTGTCCCACCTTCCAGTCTGTTTTTAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204470 representing NM_013834
Red=Cloning site Green=Tags(s) MGVGRSARGRGGAASGVLLALAAALLASGSASEYDYVSFQSDIGSYQSGRFYTKPPQCVDIPVDLRLCHN VGYKKMVLPNLLEHETMAEVKQQASSWVPLLNKNCHMGTQVFLCSLFAPVCLDRPIYPCRWLCEAVRDSC EPVMQFFGFYWPEMLKCDKFPEGDVCIAMTPPNTTEASKPQGTTVCPPCDNELKSEAIIEHLCASEFALR MKIKEVKKENGDKKIVPKKKKPLKLGPIKKKELKRLVLFLKNGADCPCHQLDNLSHNFLIMGRKVKSQYL LTAIHKWDKKNKEFKNFMKRMKNHECPTFQSVFK TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013834 |
| ORF Size | 942 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013834.2, NP_038862.2 |
| RefSeq Size | 4389 bp |
| RefSeq ORF | 945 bp |
| Locus ID | 20377 |
| UniProt ID | Q8C4U3 |
| Gene Summary | Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204216 | Sfrp1 (untagged) - Mouse secreted frizzled-related protein 1 (Sfrp1), (10ug) |
CNY 3,600.00 |
|
| MR204470 | Sfrp1 (Myc-DDK-tagged) - Mouse secreted frizzled-related protein 1 (Sfrp1) |
CNY 3,600.00 |
|
| MR204470L3 | Lenti ORF clone of Sfrp1 (Myc-DDK-tagged) - Mouse secreted frizzled-related protein 1 (Sfrp1) |
CNY 5,890.00 |
|
| MR204470L4 | Lenti ORF clone of Sfrp1 (mGFP-tagged) - Mouse secreted frizzled-related protein 1 (Sfrp1) |
CNY 6,000.00 |
