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Casp4 (NM_007609) Mouse Tagged ORF Clone
CAT＃: MG205770
- TrueORF®
Casp4 (tGFP-tagged) - Mouse caspase 4, apoptosis-related cysteine peptidase (Casp4)
ORF Plasmid: DDK
"NM_007609" in other vectors (4)
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CNY 7,088.00
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热销推荐Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Cas; CASP-4; CASP-11; Casp11; Caspa; Caspl; ich-; ich-3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205770 representing NM_007609
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAAAACAAACACCCTGACAAACCACTTAAGGTGTTGGAACAGCTGGGCAAAGAAGTCCTTACGG AGTACCTAGAAAAATTAGTACAAAGCAATGTACTGAAATTAAAGGAGGAAGATAAACAAAAATTTAACAA TGCTGAACGCAGTGACAAGCGTTGGGTTTTTGTAGATGCCATGAAAAAGAAACACAGCAAAGTAGGTGAA ATGCTTCTCCAGACATTCTTCAGTGTGGACCCAGGCAGCCACCATGGTGAAGCTAATCTGGAAATGGAGG AACCAGAAGAATCATTGAACACTCTCAAGCTTTGTTCCCCTGAAGAGTTCACAAGGCTTTGCAGAGAAAA GACACAAGAAATTTACCCAATAAAGAAGGCCAATGGCCGTACACGAAAGGCTCTTATCATATGCAATACA GAGTTCAAACATCTCTCACTGAGGTATGGGGCTAACTTTGACATCATTGGTATGAAAGGCCTTCTTGAAG ACTTAGGCTACGATGTGGTGGTGAAAGAGGAGCTTACAGCAGAGGGCATGGAGTCAGAGATGAAAGACTT TGCTGCACTCTCAGAACACCAGACATCAGACAGCACATTCCTGGTGCTAATGTCTCATGGCACACTGCAT GGCATTTGTGGAACAATGCACAGTGAAAAAACTCCAGATGTGCTACAGTATGATACCATCTATCAGATAT TCAACAATTGCCACTGTCCAGGTCTACGAGACAAACCCAAAGTCATCATTGTGCAGGCCTGCAGAGGTGG GAACTCTGGAGAAATGTGGATCAGAGAGTCTTCAAAACCCCAGTTGTGCAGAGGTGTAGATCTACCTAGG AATATGGAAGCTGATGCTGTCAAGCTGAGCCACGTGGAGAAGGACTTCATTGCCTTCTACTCTACAACCC CACATCACTTGTCCTACCGAGACAAAACAGGAGGCTCTTACTTCATCACTAGACTCATTTCCTGCTTCCG GAAACATGCTTGCTCTTGTCATCTCTTTGATATATTCCTGAAGGTGCAACAATCATTTGAAAAGGCAAGT ATTCATTCCCAGATGCCCACCATTGATCGGGCAACCTTGACGAGATATTTCTACCTCTTTCCTGGCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205770 representing NM_007609
Red=Cloning site Green=Tags(s) MAENKHPDKPLKVLEQLGKEVLTEYLEKLVQSNVLKLKEEDKQKFNNAERSDKRWVFVDAMKKKHSKVGE MLLQTFFSVDPGSHHGEANLEMEEPEESLNTLKLCSPEEFTRLCREKTQEIYPIKKANGRTRKALIICNT EFKHLSLRYGANFDIIGMKGLLEDLGYDVVVKEELTAEGMESEMKDFAALSEHQTSDSTFLVLMSHGTLH GICGTMHSEKTPDVLQYDTIYQIFNNCHCPGLRDKPKVIIVQACRGGNSGEMWIRESSKPQLCRGVDLPR NMEADAVKLSHVEKDFIAFYSTTPHHLSYRDKTGGSYFITRLISCFRKHACSCHLFDIFLKVQQSFEKAS IHSQMPTIDRATLTRYFYLFPGN TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007609 |
| ORF Size | 1119 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007609.1, NP_031635.1 |
| RefSeq Size | 1433 bp |
| RefSeq ORF | 1122 bp |
| Locus ID | 12363 |
| UniProt ID | P70343 |
| Gene Summary | This gene encodes a member of the cysteine proteases that plays important roles in apoptosis, cell migration and the inflammatory response. The encoded protein mediates production of pro-inflammatory cytokines by macrophages upon bacterial infection. Mice lacking the encoded protein are resistant to endotoxic shock induced by lipopolysaccharide. A 5-bp deletion encompassing a splice acceptor junction resulting in alternate splicing and a shorter non-functional isoform in certain mouse strains has been described. Although its official nomenclature is "caspase 4, apoptosis-related cysteine peptidase", this gene and its encoded protein have historically been called caspase 11. This gene is present in a cluster of three caspase genes on chromosome 9. [provided by RefSeq, Apr 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205003 | Casp4 (untagged) - Mouse caspase 4, apoptosis-related cysteine peptidase (Casp4), (10ug) |
CNY 2,000.00 |
|
| MR205770 | Casp4 (Myc-DDK-tagged) - Mouse caspase 4, apoptosis-related cysteine peptidase (Casp4) |
CNY 5,488.00 |
|
| MR205770L3 | Lenti ORF clone of Casp4 (Myc-DDK-tagged) - Mouse caspase 4, apoptosis-related cysteine peptidase (Casp4) |
CNY 7,888.00 |
|
| MR205770L4 | Lenti ORF clone of Casp4 (mGFP-tagged) - Mouse caspase 4, apoptosis-related cysteine peptidase (Casp4) |
CNY 7,888.00 |
