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Mvk (NM_023556) Mouse Tagged ORF Clone
CAT＃: MG206185
- TrueORF®
Mvk (tGFP-tagged) - Mouse mevalonate kinase (Mvk)
ORF Plasmid: DDK
"NM_023556" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2310010A05Rik; AI256848; AI414037; MK |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206185 representing NM_023556
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGTCAGAAGCCCTGCTGGTGTCCGCCCCGGGGAAGGTCATCCTCCATGGAGAACACGCTGTGGTCC ATGGCAAGGTCGCTCTGGCAGCGGCCTTGAACTTGAGAACTTTCCTCCTGCTGCGACCGCAGAGCAATGG GAAAGTGAGCGTCAATTTACCCAACATCGGTATTAAGCAGGTGTGGGATGTGGGCATGCTTCAGCGACTG GACACGAGCTTTCTTGAGCAAGGTGATGTCTCGGTACCCACCTTGGAGCAACTGGAGAAGCTAAAGAAGA TGGGGGACCTCCCCAGAGACCGTGCAGGCAATGAAGGCATGGCTCTGCTTGCCTTTCTCTACCTGTACCT GGCAATCTGCCGGAAGCAGAGGACACTCCCGAGCCTGGACATGGTGGTGTGGTCGGAACTTCCCCCCGGG GCAGGCTTGGGCTCCAGCGCCGCCTACTCTGTGTGTCTGGCAGCCGCCCTCCTGACTGCCTGTGAGGAGG TCTCCAACCCGCTCAAGGACGGGGTCTCCGTCAGCAGGTGGCCCGAGGAAGATCTGAAGTCAATCAACAA GTGGGCCTTCGAAGGGGAGAGAGTGATCCATGGGAACCCTTCTGGTGTGGACAATGCCGTCAGCACCTGG GGCGGAGCCCTGCGCTTCCAGCAAGGGACGATGTCTTCCTTGAAGAGCCTCCCGTCTCTGCAGATCCTGC TCACCAACACCAAGGTCCCGCGGAGTACCAAGGCCCTTGTGGCTGCTGTCAGAAGCAGGCTGACCAAGTT CCCTGAGATTGTGGCCCCGCTGCTGACCTCCATTGACGCAATATCCCTGGAGTGTGAGCGCGTGCTAGGG GAGATGGTGGCAGCTCCAGTTCCGGAACAGTACCTCGTACTAGAAGAGCTGATAGACATGAACCAGCACC ATCTGAATGCTCTCGGGGTGGGCCACAACTCCCTGGACCAGCTCTGCCAAGTAACGGCAGCACACGGACT GCACAGCAAGCTGACGGGCGCTGGCGGTGGCGGCTGTGGCATCACCCTCCTGAAGCCAGGTCTAGAGCAA GCCACAGTGGAGGCAGCCAAGCAGGCCCTGACCAGCTGCGGGTTTGACTGCTGGGAGACCAGCATCGGCG CACCCGGAGTTTCCACACACTCAGCTGCAGCTGTAGGGGACCCTGTCCGACAAGCCCTGGGCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206185 representing NM_023556
Red=Cloning site Green=Tags(s) MLSEALLVSAPGKVILHGEHAVVHGKVALAAALNLRTFLLLRPQSNGKVSVNLPNIGIKQVWDVGMLQRL DTSFLEQGDVSVPTLEQLEKLKKMGDLPRDRAGNEGMALLAFLYLYLAICRKQRTLPSLDMVVWSELPPG AGLGSSAAYSVCLAAALLTACEEVSNPLKDGVSVSRWPEEDLKSINKWAFEGERVIHGNPSGVDNAVSTW GGALRFQQGTMSSLKSLPSLQILLTNTKVPRSTKALVAAVRSRLTKFPEIVAPLLTSIDAISLECERVLG EMVAAPVPEQYLVLEELIDMNQHHLNALGVGHNSLDQLCQVTAAHGLHSKLTGAGGGGCGITLLKPGLEQ ATVEAAKQALTSCGFDCWETSIGAPGVSTHSAAAVGDPVRQALGL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023556 |
| ORF Size | 1185 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_023556.3 |
| RefSeq Size | 1712 bp |
| RefSeq ORF | 1188 bp |
| Locus ID | 17855 |
| UniProt ID | Q9R008 |
| Gene Summary | This gene encodes mevalonate kinase, a key enzyme involved in the biosynthesis of cholesterol and non-sterol isoprenes. The complete lack of encoded protein is lethal to mouse embryos. Mice lacking one allele of this gene exhibit increased levels of mevalonate in spleen, heart and kidney, as well as increased levels of serum immunoglobulins A and D. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200602 | Mvk (untagged) - Mouse mevalonate kinase (Mvk), (10ug) |
CNY 2,000.00 |
|
| MR206185 | Mvk (Myc-DDK-tagged) - Mouse mevalonate kinase (Mvk) |
CNY 3,990.00 |
|
| MR206185L3 | Lenti ORF clone of Mvk (Myc-DDK-tagged) - Mouse mevalonate kinase (Mvk) |
CNY 5,890.00 |
|
| MR206185L4 | Lenti ORF clone of Mvk (mGFP-tagged) - Mouse mevalonate kinase (Mvk) |
CNY 5,890.00 |
