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Rbm22 (NM_025776) Mouse Tagged ORF Clone
CAT＃: MG206662
- TrueORF®
Rbm22 (tGFP-tagged) - Mouse RNA binding motif protein 22 (Rbm22)
ORF Plasmid: DDK
"NM_025776" in other vectors (4)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 8430430L24Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206662 representing NM_025776
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACTTCTCTGGGTTCCAACACCTACAACAGGCAGAACTGGGAGGACGCGGACTTCCCCATTCTGT GTCAGACGTGTCTTGGAGAAAACCCTTATATACGAATGACCAAAGAAAAATATGGAAAAGAATGCAAAAT CTGTGCCAGGCCATTCACAGTGTTTCGCTGGTGCCCTGGGGTCCGCATGCGCTTCAAGAAGACTGAAGTG TGCCAAACCTGCAGTAAATTGAAGAATGTTTGTCAGACCTGCCTGTTAGACCTTGAATATGGCCTACCCA TCCAGGTTCGTGATGCAGGATTATCATTTAAAGATGACATGCCTAAGTCAGATGTCAATAAAGAATATTA CACCCAAAATATGGAAAGAGAAATTTCTAACTCTGATGGAACACGACCAGTTGGGATGCTAGGAAAAGCA ACATCCACCAGTGACATGCTCCTCAAGTTGGCCCGGACCACACCCTACTACAAAAGGAATCGGCCCCACA TTTGTTCCTTTTGGGTGAAAGGAGAGTGTAAGAGAGGAGAGGAGTGCCCATACAGACATGAGAAGCCTAC CGACCCAGACGACCCTCTTGCTGATCAGAATATCAAAGACCGGTACTATGGAATCAACGACCCCGTGGCT GACAAGCTTCTAAAGCGGGCCTCGACCATGCCCCGCCTGGACCCACCGGAGGATAAGACCATTACCACCC TGTACGTCGGCGGTCTGGGAGACACCATCACCGAAACAGATCTCAGAAATCATTTCTACCAGTTTGGAGA GATCCGCACCATCACTGTTGTGCAGAGGCAGCAGTGTGCCTTCATCCAGTTCGCCACCAGGCAGGCTGCC GAGGTAGCCGCTGAGAAGTCCTTTAACAAGCTGATCGTGAATGGCCGGAGGCTCAATGTCAAGTGGGGAA GATCCCAAGCAGCCAGAGGCAAAGAAAAAGAGAAGGATGGAACCACAGACTCTGGGATCAAGCTAGAACC TGTGCCAGGACTGCCTGGAGCTCTGCCTCCACCTCCTGCAGCAGAAGAGGAGGCGTCTGCCAACTACTTC AACCTGCCCCCAAGTGGTCCTCCAGCAGTGGTGAACATTGCCCTGCCCCCACCCCCTGGGATCGCCCCAC CGCCACCCCCAGGTTTTGGACCACACATGTTCCACCCCATGGGACCACCCCCTCCTTTCATGAGGGCTCC AGGACCAATCCACTACCCTTCTCAGGACCCTCAGAGAATGGGTGCTCATGCAGGAAAACACAGCAGCCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206662 representing NM_025776
Red=Cloning site Green=Tags(s) MATSLGSNTYNRQNWEDADFPILCQTCLGENPYIRMTKEKYGKECKICARPFTVFRWCPGVRMRFKKTEV CQTCSKLKNVCQTCLLDLEYGLPIQVRDAGLSFKDDMPKSDVNKEYYTQNMEREISNSDGTRPVGMLGKA TSTSDMLLKLARTTPYYKRNRPHICSFWVKGECKRGEECPYRHEKPTDPDDPLADQNIKDRYYGINDPVA DKLLKRASTMPRLDPPEDKTITTLYVGGLGDTITETDLRNHFYQFGEIRTITVVQRQQCAFIQFATRQAA EVAAEKSFNKLIVNGRRLNVKWGRSQAARGKEKEKDGTTDSGIKLEPVPGLPGALPPPPAAEEEASANYF NLPPSGPPAVVNIALPPPPGIAPPPPPGFGPHMFHPMGPPPPFMRAPGPIHYPSQDPQRMGAHAGKHSSP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025776 |
| ORF Size | 1260 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025776.2 |
| RefSeq Size | 2080 bp |
| RefSeq ORF | 1263 bp |
| Locus ID | 66810 |
| UniProt ID | Q8BHS3 |
| Gene Summary | Required for pre-mRNA splicing as component of the activated spliceosome. Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202792 | Rbm22 (untagged) - Mouse RNA binding motif protein 22 (Rbm22), (10ug) |
CNY 2,000.00 |
|
| MR206662 | Rbm22 (Myc-DDK-tagged) - Mouse RNA binding motif protein 22 (Rbm22) |
CNY 2,850.00 |
|
| MR206662L3 | Lenti ORF clone of Rbm22 (Myc-DDK-tagged) - Mouse RNA binding motif protein 22 (Rbm22) |
CNY 4,750.00 |
|
| MR206662L4 | Lenti ORF clone of Rbm22 (mGFP-tagged) - Mouse RNA binding motif protein 22 (Rbm22) |
CNY 4,750.00 |
