9630058J23Rik (BC034107) Mouse Tagged ORF Clone
CAT＃: MG206713
- TrueORF®
9630058J23Rik (tGFP-tagged) - Mouse RIKEN cDNA 9630058J23 gene (cDNA clone MGC:31547 IMAGE:4503249)
ORF Plasmid: DDK
"BC034107" in other vectors (3)
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CNY 4560.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MGC31547 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206713 representing BC034107
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGAAGAGGCAGTGGGGACAGAAGCAGCAGCCAAAGAGCCAGAGATTGAAACCTGTCCCAGCACGG ATCCAAGTGGAGACCGGCATGAGGAGGAGCCCCAGGAGAGCAGCCCCGGCTGTCATCAGATGGAGTGGCA GACAGCTTCCCCCGAGCTTCCAGGGACTGCAGGAAAGGACCACACGGAGGAGCTGCCCAGCAGCACCAAT GCCACACTGGACCTCCACACCCAGTCCCTGGAGACAGCCGGGAGCAGGTCAGGGCCAGCTGCTGCCTCGA ATGCCTGCAAAGACAGCAGCTGCGTGCCGGCGCCTCCCACAGAGGACCACTGCGGTGTGGCCAGAGACCC TAAGGTGGCTCCTCCTAGTGAATCAGTGGCAGAGCAGAAGCTCTCCACCGGCGACGACGGTGCACTGCCT GGGTTGATTTCAGAGGGTAAATATTCACAGGCACACCGAAAAGAACTCTGCCTTCCTCTGCAGGACGCAT TTGAAGCGCTGCCCAGGGACCAGCCTCACAGCAGTGAGGTAGCTGAGCCGCGGCAGCCTGATGTCACGGC CAGTGATGGGAAATCAGCACAGAGTCAGGCTGGCTTGGAGACTGGTCCTGAGAGTGCACTCTGTGGTGAC AGGAAAGCCTGTGACGTAAGCACACTGTGTCTGGAAGTGTGTATGGCCCCAGAAGAAAGGCGGGACAGTG AAGACCGAGTGAGTAAGGAAACGGAAGACTATCTGCACAGCCTTTTGGAGAGATGCTTGAAGGATGCTGA AGATTCCCTGTCCTACGAGGACATCCAGGACGACGACTCTGACCTCCTCCAAGACCTCTCTCCTGAAGAA GCGTCCTACAGTCTCCAGGAGGATCTGCCTCCTGACGAGAGCACGCTCTCCCTTGATGATCTGGCCAAGA AGATAGAGATCGCAGAGGCCATTCCTGCAGAAGGGCTGGTGTCCATACTGAAGAAGAGGAATGACACAGT AGGCAGCCATCCTGCACAGATGCAGCAGAAGCCGGCTAAGCGCAGAGTGCGATTCCAGGAAATAGACGAC AACCTGGAGCAAGATGAGGTTGGTGGTGGTTCCTGCATCCTGCTCATCTTGCTGTGCATAGCCACTGTCT TCCTCAGTGTTGGAGGAACTGCACTCTACTGCACCCTGGGCAACATTGAGTCACCTGTTTGCACGGACTT TGCCGACAACGTGGACTTCTATTACACTAAGCTGCTGCAGGGGGTGGCAGGGCTGAAACACTGGGTCTAC CTCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206713 representing BC034107
Red=Cloning site Green=Tags(s) MSEEAVGTEAAAKEPEIETCPSTDPSGDRHEEEPQESSPGCHQMEWQTASPELPGTAGKDHTEELPSSTN ATLDLHTQSLETAGSRSGPAAASNACKDSSCVPAPPTEDHCGVARDPKVAPPSESVAEQKLSTGDDGALP GLISEGKYSQAHRKELCLPLQDAFEALPRDQPHSSEVAEPRQPDVTASDGKSAQSQAGLETGPESALCGD RKACDVSTLCLEVCMAPEERRDSEDRVSKETEDYLHSLLERCLKDAEDSLSYEDIQDDDSDLLQDLSPEE ASYSLQEDLPPDESTLSLDDLAKKIEIAEAIPAEGLVSILKKRNDTVGSHPAQMQQKPAKRRVRFQEIDD NLEQDEVGGGSCILLILLCIATVFLSVGGTALYCTLGNIESPVCTDFADNVDFYYTKLLQGVAGLKHWVY LS TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC034107 |
| ORF Size | 1266 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC034107.1 |
| RefSeq Size | 3579 bp |
| RefSeq ORF | 1268 bp |
| Locus ID | 226744 |
| Gene Summary | Required for targeting of connexins to the plasma membrane.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR206713 | 9630058J23Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 9630058J23 gene (cDNA clone MGC:31547 IMAGE:4503249) |
CNY 4180.00 |
|
| MR206713L3 | Lenti ORF clone of 9630058J23Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 9630058J23 gene (cDNA clone MGC:31547 IMAGE:4503249) |
CNY 6080.00 |
|
| MR206713L4 | Lenti ORF clone of 9630058J23Rik (mGFP-tagged) - Mouse RIKEN cDNA 9630058J23 gene (cDNA clone MGC:31547 IMAGE:4503249) |
CNY 6080.00 |
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