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Kcnn4 (NM_008433) Mouse Tagged ORF Clone
CAT＃: MG206777
- TrueORF®
Kcnn4 (tGFP-tagged) - Mouse potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (Kcnn4)
ORF Plasmid: DDK
"NM_008433" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | IK1; IKCA1; KCa3.1; KCA4; mIKCa1; SK4; SKCas |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206777 representing NM_008433
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGGGGAGCTGGTGACTGGCCTGGGGGCCCTGAGACGGAGAAAGCGCCTGCTGGAGCAGGAGAAGA GGGTGGCCGGCTGGGCGTTGGTGCTGGCGGGAACTGGCATCGGACTCATGGTTCTGCACGCTGAGATGTT GTGGTTCCTGGGCTGCAAGTGGGTGCTGTACCTGCTCCTGGTTAAGTGTTTGATCACCCTGTCCACTGCC TTCCTCCTTTGTCTTATTGTGGTCTTCCATGCCAAGGAGGTCCAGCTGTTCATGACTGACAACGGGCTCC GGGACTGGCGCGTGGCGCTGACCCGGCGGCAGGTGGCGCAGATCCTGCTGGAGCTGTTGGTGTGCGGGGT GCACCCGGTGCCCCTACGGAGCCCGCACTGCGCCCTGGCGGGGGAGGCCACCGACGCGCAGCCCTGGCCG GGTTTCCTGGGCGAAGGCGAGGCGTTGCTGTCCCTGGCCATGCTCCTGCGTCTCTACCTGGTGCCCCGCG CGGTGCTGCTGCGCAGCGGGGTCCTGCTCAACGCGTCCTACCGCAGCATCGGGGCGCTCAACCAAGTCCG CTTCCGCCACTGGTTCGTGGCCAAGCTGTACATGAACACGCACCCGGGTCGCCTGCTGCTGGGCCTCACG CTGGGTCTCTGGCTCACCACAGCTTGGGTGCTGTCTGTGGCTGAGAGGCAGGCTGTCAATGCCACGGGGC ACCTCACAGACACACTGTGGCTGATTCCGATCACATTCCTGACCATTGGCTATGGGGACGTGGTACCTGG CACCATGTGGGGCAAGATTGTCTGCCTGTGCACCGGAGTCATGGGGGTCTGCTGCACAGCTCTCCTGGTG GCTGTGGTGGCTCGGAAGCTGGAGTTCAACAAGGCGGAGAAACACGTGCACAACTTCATGATGGACATCC ATTATGCCAAAGAGATGAAGGAGTCAGCGGCGCGGCTGCTGCAGGAAGCCTGGATGTACTACAAGCACAC TCGAAGGAAGGACTCCCGGGCTGCCCGCAGACATCAGCGCAAGATGCTGGCCGCCATCCACACGTTCCGC CAGGTACGGCTGAAACACCGGAAGCTCCGGGAACAAGTGAATTCCATGGTGGACATCTCCAAGATGCACA TGATCCTGTGCGACCTGCAGCTGGGTCTCAGCTCCTCGCACCGTGCCCTGGAGAAGAGAATCGACGGTCT GGCAGGAAAGCTGGATGCCCTGACAGAGCTGCTCGGCACTGCCCTGCAGCAACAGCAGCTACCAGAACCC AGCCAGGAGGCCACA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206777 representing NM_008433
Red=Cloning site Green=Tags(s) MGGELVTGLGALRRRKRLLEQEKRVAGWALVLAGTGIGLMVLHAEMLWFLGCKWVLYLLLVKCLITLSTA FLLCLIVVFHAKEVQLFMTDNGLRDWRVALTRRQVAQILLELLVCGVHPVPLRSPHCALAGEATDAQPWP GFLGEGEALLSLAMLLRLYLVPRAVLLRSGVLLNASYRSIGALNQVRFRHWFVAKLYMNTHPGRLLLGLT LGLWLTTAWVLSVAERQAVNATGHLTDTLWLIPITFLTIGYGDVVPGTMWGKIVCLCTGVMGVCCTALLV AVVARKLEFNKAEKHVHNFMMDIHYAKEMKESAARLLQEAWMYYKHTRRKDSRAARRHQRKMLAAIHTFR QVRLKHRKLREQVNSMVDISKMHMILCDLQLGLSSSHRALEKRIDGLAGKLDALTELLGTALQQQQLPEP SQEAT SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008433 |
| ORF Size | 1275 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008433.2, NP_032459.2 |
| RefSeq Size | 1951 bp |
| RefSeq ORF | 1278 bp |
| Locus ID | 16534 |
| UniProt ID | O89109 |
| Gene Summary | Forms a voltage-independent potassium channel that is activated by intracellular calcium (PubMed:9705284). Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells (PubMed:20884616). Plays a role in the late stages of EGF-induced macropinocytosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200643 | Kcnn4 (untagged) - Mouse potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (Kcnn4), transcript variant 1, (10ug) |
CNY 2,000.00 |
|
| MR206777 | Kcnn4 (Myc-DDK-tagged) - Mouse potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (Kcnn4), transcript variant 1 |
CNY 3,330.00 |
|
| MR206777L3 | Lenti ORF clone of Kcnn4 (Myc-DDK-tagged) - Mouse potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (Kcnn4), transcript variant 1 |
CNY 5,230.00 |
|
| MR206777L4 | Lenti ORF clone of Kcnn4 (mGFP-tagged) - Mouse potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (Kcnn4), transcript variant 1 |
CNY 5,230.00 |
