Saal1 (BC087944) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	5031425D22Rik; SPACIA1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207593 representing BC087944 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCGAAACCCGTCTCCTCCGCCGCCTACCTGTGGTTCGGAGGATGAAGAGGACCTGGGTGGGGGAG ACCGCATCGGGAGCACGGTCTACAGCAAGCACTGGCTCTTCGGCGTCCTCAGCGGGCTCATCCAGATTGT CACACCTGAAAGTGGCACATCTGGCTCAGCTGATGAGGAAGAACAGGCAGATCTTGCTGAAGAAATGGAG AATGAGATTTGCAGAGTCTGGGATATGTCCATGGACGAGGATGTGGCTTTATTTCTCCAAGAATTTAAGG CTCCTGACATATTCATGGGAGTGCTGGCCAAATCCCCGTGTCCCCGATTAAGAGAAATCTGTGTGGGAAT TTTAGGGAATATGGCTTGTTTCCGGGAGATATGCGAGTCCATCAGCAAGAACGAGGATCATGGGCAGGTG CTGCTGCAGTGTTTGTGTGACTCAGACCCACCCACCCTGCTGGAAACTTGCAGGTTGTTGCTTACTTGCC TTTCCCAGACAGAAGTGGCCAGTGTCTGGGTTAGAAGGATCCGGGAACACCCATCTGTCTACGCTAACGT TTGCTTCATCATGTCCAGTTCAACAAACGTTGACTTGCTGGTGAAGGTCGGGGAGGTCGTGGACAAACTC TTTGACTTAGATGAAAAACTCATGTTGGAATGGATTAGAAAGGGGGCTACCCGGCTTCCAGGCCAACCCC ACGAGGACTCTGAAGAGCAGCCAGTGTTTTCCATTGTACCCTGTGTGCTGGAAGCTGCCAAACAAGTGCG CTCTGAAAACCTGGAGGGGCTGGATGTTTACATGCGCATCTTACAGCTGCTCACCACGGTGGATGACGGC GTGCAAGCCATCGTGCAGTGTCCTGATACTGGAAACGACACGTGGCGTTTACTCTTTGACCTGGTGTGCC ATGAATTCTGCCAGCCTGATGACCCGCCTGTCATACTGCAGGAACAGAAGACAGTGCTAGCGTCTGTCTT CTCAGTGCTGTCAGCCATCAGTGCCTCACGGGCCGAACAGGAGCACCTGAAGATAGAAGAAGGGGATCTT CCTCTAATTGACAGCCTAATTCGGGTATTACAAAATATGGAACATTGTCAGAAGAAACCAGAGAACCCCT CAGAGTCTGATACAGAAGAGCCTACGATCTGTGGTCCCACCCAAGACGACTTCCATATGAAGATCTTGAA GGACATTTCATGTGAATTCCTTTCTAATATTTTTCAGGTGTTAACAAAGGAAAAGGTGGCTCAGGGACTG AAGGAGGGCCAGTTAAGCAAGCAGAAGTGCTCCTGCGCCTTTCAGAGCCTCCTGCCCCTCTACGGCCCTG CAGTGGAAGACTTCGTTAAAGTCGTACGTGAGGTCGATGAGGCTCTTGCTGATGACCTAGAAGACAGCTT CCCAAGTGTGAAGGCACAGACT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207593 representing BC087944 Red=Cloning site Green=Tags(s) MDRNPSPPPPTCGSEDEEDLGGGDRIGSTVYSKHWLFGVLSGLIQIVTPESGTSGSADEEEQADLAEEME NEICRVWDMSMDEDVALFLQEFKAPDIFMGVLAKSPCPRLREICVGILGNMACFREICESISKNEDHGQV LLQCLCDSDPPTLLETCRLLLTCLSQTEVASVWVRRIREHPSVYANVCFIMSSSTNVDLLVKVGEVVDKL FDLDEKLMLEWIRKGATRLPGQPHEDSEEQPVFSIVPCVLEAAKQVRSENLEGLDVYMRILQLLTTVDDG VQAIVQCPDTGNDTWRLLFDLVCHEFCQPDDPPVILQEQKTVLASVFSVLSAISASRAEQEHLKIEEGDL PLIDSLIRVLQNMEHCQKKPENPSESDTEEPTICGPTQDDFHMKILKDISCEFLSNIFQVLTKEKVAQGL KEGQLSKQKCSCAFQSLLPLYGPAVEDFVKVVREVDEALADDLEDSFPSVKAQT TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC087944
ORF Size	1422 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC087944, AAH87944
RefSeq Size	1540 bp
RefSeq ORF	1424 bp
Locus ID	78935
Gene Summary	Plays a role in promoting the proliferation of synovial fibroblasts in response to proinflammatory stimuli.[UniProtKB/Swiss-Prot Function]

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