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Clk2 (NM_007712) Mouse Tagged ORF Clone
CAT＃: MG207997
- TrueORF®
Clk2 (tGFP-tagged) - Mouse CDC-like kinase 2 (Clk2)
"NM_007712" in other vectors (5)
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CNY 5,230.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AU041688 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207997 representing NM_007712
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCATCCCCGAAGGTACCATTCCTCAGAGCGAGGTAGCCGGGGGAGTTACCACGAACACTATCGGA GCCGAAAGCATAAGCGAAGAAGAAGTCGCTCCTGGTCAAGTAGCAGTGACCGGACAAGGCGGCGGCGGAG GGAGGACAGCTACCACGTTCGGTCCCGAAGCTATGATGACCATTCGTCCGATCGGCGGCTGTACGATCGG CGGTACTGTGGCAGCTACAGGCGCAATGACTACAGCCGGGACAGAGGGGAGGCTTACTACGACACAGACT TTCGGCAGTCCTATGAATACCATCGAGAGAACAGCAGTTACCGAAGCCAGCGCAGCAGCCGAAGGAAACA CAGAAGGCGGAGGAGACGGAGCCGGACATTCAGCCGCTCATCTTCACACAGCAGCCGGAGAGCCAAGAGT GTAGAGGACGACGCTGAGGGCCACCTCATCTACCACGTCGGGGACTGGCTACAAGAGCGATATGAAATTG TAAGCACCTTAGGAGAAGGGACCTTCGGCCGAGTTGTGCAGTGTGTGGACCATCGCAGGGGCGGAACACG AGTTGCCCTGAAGATCATTAAGAATGTGGAAAAGTACAAGGAAGCAGCCCGACTAGAAATCAACGTGCTG GAGAAAATCAATGAGAAAGATCCTGACAACAAGAACCTCTGTGTCCAGATGTTTGACTGGTTTGACTACC ATGGCCACATGTGTATCTCCTTTGAGCTTCTGGGCCTTAGCACCTTCGATTTCCTCAAAGACAACAACTA CCTGCCCTACCCCATCCACCAAGTGCGCCACATGGCCTTCCAGCTCTGCCAGGCCGTCAAGTTCCTCCAT GATAACAAGTTGACACATACGGACCTCAAACCTGAAAATATTCTGTTTGTGAATTCAGACTACGAACTCA CCTACAACCTAGAGAAGAAGCGAGATGAGCGCAGTGTAAAGAGCACAGCCGTGCGGGTGGTGGACTTCGG CAGTGCCACCTTTGACCATGAACACCATAGCACCATTGTCTCCACTCGCCATTACCGAGCCCCCGAGGTC ATCCTGGAGTTGGGCTGGTCACAGCCATGCGATGTATGGAGCATAGGCTGCATCATCTTTGAGTACTACG TTGGCTTCACCCTCTTCCAGACCCATGACAACAGAGAGCATCTAGCCATGATGGAAAGGATCCTGGGTCC TGTCCCTTCTCGGATGATCAGAAAGACAAGAAAACAGAAATATTTTTATCGGGGTCGCCTGGATTGGGAT GAGAACACCTCAGCCGGGCGCTACGTTCGTGAGAACTGCAAACCTCTGCGGCGGTATCTGACCTCAGAGG CAGAGGACCACCACCAGCTCTTCGATCTGATTGAAAATATGCTAGAGTATGAGCCTGCTAAGCGGCTGAC CTTAGGTGAAGCCCTTCAGCATCCTTTCTTCGCCTGCCTTCGGACTGAGCCACCCAACACCAAGTTGTGG GACTCCAGTCGGGATATCAGTCGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207997 representing NM_007712
Red=Cloning site Green=Tags(s) MPHPRRYHSSERGSRGSYHEHYRSRKHKRRRSRSWSSSSDRTRRRRREDSYHVRSRSYDDHSSDRRLYDR RYCGSYRRNDYSRDRGEAYYDTDFRQSYEYHRENSSYRSQRSSRRKHRRRRRRSRTFSRSSSHSSRRAKS VEDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGTRVALKIIKNVEKYKEAARLEINVL EKINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYPIHQVRHMAFQLCQAVKFLH DNKLTHTDLKPENILFVNSDYELTYNLEKKRDERSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEV ILELGWSQPCDVWSIGCIIFEYYVGFTLFQTHDNREHLAMMERILGPVPSRMIRKTRKQKYFYRGRLDWD ENTSAGRYVRENCKPLRRYLTSEAEDHHQLFDLIENMLEYEPAKRLTLGEALQHPFFACLRTEPPNTKLW DSSRDISR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007712 |
| ORF Size | 2307 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007712.1 |
| RefSeq Size | 1538 bp |
| RefSeq ORF | 1500 bp |
| Locus ID | 12748 |
| UniProt ID | O35491 |
| Gene Summary | Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205713 | Clk2 (untagged) - Mouse CDC-like kinase 2 (Clk2), transcript variant 1, (10ug) |
CNY 2,000.00 |
|
| MG223242 | Clk2 (tGFP-tagged) - Mouse CDC-like kinase 2 (Clk2) transcript variant 1, (10ug) |
CNY 5,420.00 |
|
| MR223242 | Clk2 (Myc-DDK-tagged) - Mouse CDC-like kinase 2 (Clk2), transcript variant 1 |
CNY 4,940.00 |
|
| MR223242L3 | Lenti ORF clone of Clk2 (Myc-DDK-tagged) - Mouse CDC-like kinase 2 (Clk2), transcript variant 1 |
CNY 6,840.00 |
|
| MR223242L4 | Lenti ORF clone of Clk2 (mGFP-tagged) - Mouse CDC-like kinase 2 (Clk2), transcript variant 1 |
CNY 6,840.00 |
