Rftn2 (BC038341) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2700010E02Rik; 3222401M22Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208011 representing BC038341 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTGCGGACTTAGGAAGCTGGAAGACCCTGATGAAAGCAGCCCTGGAAAAATATTTTCTACCCTGA AGAGACCGCAAGTGGAAACAAAGACAGAATTTGCTTATGAATACGCCCTGCTGGACTTTACTTTACAAGC TTCAACAAATCCAGACGTCATCAAGATAAATTCAGTCCTGGATATAGTTGCCAAAGTGGAAGACTATTAT CTTAAGGGCTATGTTGTTGGGGCCATTCACCCAGTTATACAACCTGTGGGGCAGCGGAAGCACCTTCCAG CAAGTCACCTGTACAGGGCGGTGCTGTCCCGGCTGAAGCTAAGCCCCAAGCATTCTGCAGCAGGCGGACA AAGGCGCGCAAGGCTTGTGATGGAGGAGTGTCCACTAACTTGTGAGGCACAAGCAAACGACGCTGCAAAG GAACTCATGGACAAGATTAATGCCGCTGCTAAAAGAGGGATGAAGTTTGTTGGGCTGGTCTCCCAGTGCT ACTTGCCATCTATGCACTGTAATGGAGCCAGCCACGATGGAGTTGCAGAGTCGGGGCTACACGTGAGACA AGATTCACAAGACAACTGCAAAGGCTGGAATGAAGGAGCCCTTGGTGGACACTTGTCTGAAAGTGGAGTG GAGGAGGAACCTCAGCATGAAAGTGGACAGCATCAGACAGAGAGGAACAGCAGCCCCAGCTACGCTAACC CAAAGCGGGGAGAAGCCCCAGATGGTAAGCTGTACATGGTCTTCAATGCTTTTGAGGAAGATGCGGCCAG CTGGGCCTATCAGGAAGGTGTCCTGTCCATGAAAGTGACGCGGAAGGGAGCTGTCATCAGCGCTCTGGAC GCCAACTGGCTAGAACTAACGACATTTTACTATAAGCAAGGCTTCTCTTTAATTGACTCTTTTGTGTGCT GGGAGACACCTAAAGGGGACCAGTTACCTAAATCTTTGGAAGGCTTTTTTATCTACGAAGAAGAAGGATC TGGAGTCCCAGGTTCTAACAGGAGAGGAAACGACGCCATTGTGGTGGAGCAGTGGACAGTCATTGAGGGC TGTGAAATTAAAACAGATTACGGCCCCCTGCTGCACACCCTGGCTGAGTTTGGATGGCTTCTGACAAGCG TGTTGCCTACCCCGATATTGAGACATGACAGTGAAGGGAATTTGGCTACCAAGCAAGTCGTATTTCTCCA AAGGCCGGTTACGTGGAACTCAGCTGCTCAAACCCCAGAAAGGAAAGGCAGCCGGCTGCTGAAGGGTGAA GACAGAAACAAGGTCAGCAGCAGGAGCCTAGGCCTGGACACAAATGCATCCCAAGCCGCAGGAGGCCGAG CCCCTCTTGAGGAGGGCTCCCTCTCTCCGTCCAGGGAGTGCTGGACCAAGGAAGAGCGGCCCGCACAGAG CGACAGCTTCTCTGGGTTCAGTAGCAGTGACAGTGTCCTCCGGGAACTCGATGACGGACAGTTTGACCAG GAAGAGGGCGTGACACAGGTCACTTGCATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208011 representing BC038341 Red=Cloning site Green=Tags(s) MGCGLRKLEDPDESSPGKIFSTLKRPQVETKTEFAYEYALLDFTLQASTNPDVIKINSVLDIVAKVEDYY LKGYVVGAIHPVIQPVGQRKHLPASHLYRAVLSRLKLSPKHSAAGGQRRARLVMEECPLTCEAQANDAAK ELMDKINAAAKRGMKFVGLVSQCYLPSMHCNGASHDGVAESGLHVRQDSQDNCKGWNEGALGGHLSESGV EEEPQHESGQHQTERNSSPSYANPKRGEAPDGKLYMVFNAFEEDAASWAYQEGVLSMKVTRKGAVISALD ANWLELTTFYYKQGFSLIDSFVCWETPKGDQLPKSLEGFFIYEEEGSGVPGSNRRGNDAIVVEQWTVIEG CEIKTDYGPLLHTLAEFGWLLTSVLPTPILRHDSEGNLATKQVVFLQRPVTWNSAAQTPERKGSRLLKGE DRNKVSSRSLGLDTNASQAAGGRAPLEEGSLSPSRECWTKEERPAQSDSFSGFSSSDSVLRELDDGQFDQ EEGVTQVTCM TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC038341
ORF Size	1500 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC038341, AAH38341
RefSeq Size	3689 bp
RefSeq ORF	1502 bp
Locus ID	74013
Gene Summary	Upon bacterial lipopolysaccharide stimulation, mediates clathrin-dependent internalization of TLR4 in dendritic cells, resulting in activation of TICAM1-mediated signaling and subsequent IFNB1 production (PubMed:27022195). May regulate B-cell antigen receptor-mediated signaling.[UniProtKB/Swiss-Prot Function]

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