Vangl2 (NM_033509) Mouse Tagged ORF Clone

CAT#: MG208343

  • TrueORF®

Vangl2 (tGFP-tagged) - Mouse vang-like 2 (van gogh, Drosophila) (Vangl2)

ORF Plasmid: DDK tGFP



  "NM_033509" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 7,432.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms C530001F03Rik; loop-tail; Lootl; Lp; Lpp1; Ltap; ska17; ska; stbm; strabismus; Vang1l2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG208343 representing NM_033509
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACACCGAGTCCCAGTACTCGGGCTATTCCTACAAGTCGGGCCACTCCCGCAGCTCCCGGAAGCACA
GGGACCGCCGGGACCGACACCGCTCTAAGAGCCGGGATGGGAGTCGTGGAGATAAATCAGTGACGATCCA
GGCTCCGGGAGAACCCCTGCTGGACAATGAGTCCACGAGGGGGGATGAGCGGGATGACAACTGGGGAGAA
ACAACAACGGTGGTCACGGGCACTTCTGAGCACAGTATCTCCCATGATGACCTCACGCGCATCGCCAAGG
ACATGGAGGACAGTGTCCCGTTGGATTGTTCCCGCCACCTGGGCGTGGCGGCAGGGGCCATTCTGGCGCT
GCTCTCGTTCCTCACCCCGCTGGCTTTCCTGCTGCTGCCTCCACTGCTGTGGCGGGAGGAGCTGGAGCCG
TGTGGGACGGCCTGTGAGGGCCTCTTCATCTCCGTGGCCTTCAAGCTGCTCATCCTGCTGTTGGGCAGCT
GGGCTCTGTTCTTCCGCCGGCCCAAGGCCTCACTGCCCCGAGTCTTCGTGTTACGAGCTCTGCTCATGGT
GCTTGTCTTCCTGCTGGTTATTTCCTATTGGCTCTTCTACGGTGTGCGCATCTTGGACGCCCGGGAGCGG
AGCTACCAGGGCGTGGTTCAGTTTGCCGTTTCTCTAGTGGATGCTTTACTCTTCGTGCACTATCTGGCCG
TAGTTCTGCTGGAGCTCCGTCAGCTCCAGCCCCAGTTCACACTCAAGGTCGTGCGATCCACAGATGGGGC
CAGCCGCTTCTACAATGTCGGCCATCTCAGCATCCAGCGAGTGGCAGTGTGGATCCTGGAGAAGTATTAC
CATGACTTCCCTGTCTACAACCCCGCCCTCCTCAACCTGCCCAAGTCCGTCCTGGCCAAGAAAGTGTCTG
GCTTCAAGGTGTATTCTCTCGGAGAGGAAAACAGCACCAATAACTCCACGGGCCAATCAAGGGCTGTGAT
CGCGGCTGCGGCACGGAGGCGCGACAACAGCCACAATGAGTACTACTACGAGGAAGCCGAGCATGAGCGC
AGAGTGCGCAAGCGCAGGGCCAGGCTCGTGGTGGCTGTGGAGGAGGCCTTCACGCACATTAAGCGGCTGC
AGGAAGAGGAGCAGAAGAACCCCAGGGAGGTGATGGACCCCCGGGAAGCAGCCCAAGCGATCTTTGCATC
CATGGCTCGTGCCATGCAGAAGTACCTTCGCACCACCAAACAGCAGCCTTACCATACCATGGAGAGCATC
CTTCAGCACCTGGAGTTCTGCATTACCCACGACATGACACCCAAGGCCTTCCTGGAGCGATATTTGGCTG
CTGGACCCACCATCCAGTACCACAAGGAACGTTGGCTGGCCAAACAGTGGACCTTGGTGAGCGAGGAGCC
GGTGACCAATGGGCTTAAGGATGGCATCGTGTTCCTCTTGAAGCGCCAGGACTTCAGCTTGGTAGTGAGC
ACCAAGAAGGTGCCCTTCTTCAAACTCTCTGAGGAATTTGTGGATCCCAAGTCACATAAGTTCGTCATGC
GGCTGCAGTCGGAGACCTCTGTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG208343 representing NM_033509
Red=Cloning site Green=Tags(s)

MDTESQYSGYSYKSGHSRSSRKHRDRRDRHRSKSRDGSRGDKSVTIQAPGEPLLDNESTRGDERDDNWGE
TTTVVTGTSEHSISHDDLTRIAKDMEDSVPLDCSRHLGVAAGAILALLSFLTPLAFLLLPPLLWREELEP
CGTACEGLFISVAFKLLILLLGSWALFFRRPKASLPRVFVLRALLMVLVFLLVISYWLFYGVRILDARER
SYQGVVQFAVSLVDALLFVHYLAVVLLELRQLQPQFTLKVVRSTDGASRFYNVGHLSIQRVAVWILEKYY
HDFPVYNPALLNLPKSVLAKKVSGFKVYSLGEENSTNNSTGQSRAVIAAAARRRDNSHNEYYYEEAEHER
RVRKRRARLVVAVEEAFTHIKRLQEEEQKNPREVMDPREAAQAIFASMARAMQKYLRTTKQQPYHTMESI
LQHLEFCITHDMTPKAFLERYLAAGPTIQYHKERWLAKQWTLVSEEPVTNGLKDGIVFLLKRQDFSLVVS
TKKVPFFKLSEEFVDPKSHKFVMRLQSETSV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_033509
ORF Size 1563 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_033509.2
RefSeq Size 2260 bp
RefSeq ORF 1566 bp
Locus ID 93840
UniProt ID Q91ZD4
Gene Summary Involved in the control of early morphogenesis and patterning of both axial midline structures and the development of neural plate. Plays a role in the regulation of planar cell polarity, particularly in the orientation of stereociliary bundles in the cochlea. Required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process. Required for cell surface localization of FZD3 and FZD6 in the inner ear (PubMed:16495441).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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