Tmtc4 (BC031368) Mouse Tagged ORF Clone
CAT＃: MG208391
- TrueORF®
Tmtc4 (tGFP-tagged) - Mouse transmembrane and tetratricopeptide repeat containing 4 (cDNA clone MGC:32342 IMAGE:5029461)
ORF Plasmid: DDK
"BC031368" in other vectors (4)
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CNY 3230.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4930403J22Rik; 5730419O14Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208391 representing BC031368
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTGCAAAGAGCAAGGAATCACTGTGCTGGGCTTGAATGCTGTGTTCGATATCCTGGTGATAGGCA AACTCGACATTCTGGCAGCTGTCCGGAAGGTCCTACATAAGGACAAGTCACAAGAGAATGCAGGCATGTT CAAGAATGGAGGCCTCCTTTTCCGAATAGCCCTGCTTACCATTGGAGGCACCAGCATGCTTTATATACGG TGGAAAATCATGGGAACAGGCCCACCGGCATTTACTGAGGTGGACAACCCAGCATCCTTTGCTGACAGCA TGTTGGTTAGGGCCATAAACTATAATTACTACTATTCATTGAATGCCTGGCTGCTGCTGTGTCCCTGGTG GCTGTGTTTTGATTGGTCAATGGGCTGCATCCCCCTCATTAAGTCAGTTGGTGACTGGAGGGTAATTGCT CTAGCGGCACTTTGGCTCTGCCTAATTGGCCTAATATTCCAAGCCCTGTGCTCTGAGGATAGCTGCAAGA GAAGAATCCTTACTCTGGGTTTGGGATTTCTTGTGATCCCCTTTCTTCCTGCAAGCAACTTGTTTTTCCG AGTGGGCTTTGTGGTCGCAGAACGTGTCCTCTACCTTCCCAGCGCTGGGTACTGCGTGCTGCTGACCTTT GGATTTGGAGCTCTGAGCAGACACACTAAGAAAAAGAAACCTGTTGCCGCCATCATCCTGGGCATCCTTC TCATCAATGCACTGAGATGTGTGATACGCAGTGGTGAGTGGCGGAGTGAAGAGCAGCTCTTTCGAAGCGC CCTGTCTGTGTGTCCCCTCAATGCTAAGGTTCACTACAACATTGGCAAAAACCTGGCTGATCAAGGCAAC CAAACAGCGGCCATCAAATATTACCGGGAAGCAGTCAGACTAAATCCCAAGTACGTTCATGCCATGAATA ACCTCGGGAATATCTTAAAAGAAAGGAATGAGCTACAGGAAGCTGAAGAGCTGCTGTCGTTGGCTGTCCA AATACAGCCAGACTTTGCAGCCGCATGGATGAATTTGGGCATAGTTCAGAACAGCCTGAAACGGTTTGAG GAAGCAGAGCAGAGCTACCGGACAGCGATTAAACACAGGAGGAAATACCCAGACTGCTACTACAACCTCG GGCGGCTGTATGCAGATCTAAATCGCCACGTGGATGCATTGAACGCTTGGAGAAATGCCACCGTACTGAA GCCGGAGCACAGCCTGGCGTGGAACAACATGATCATACTTCTGGATAACACAGGTAACTTAGCCCAGGCT GAAGCTGTTGGAAGAGAGGCCCTGCAATTAATACCTAATGATCACTCCCTTATGTTCTCATTGGCAAACG TGCTGGGGAAATCCCAGAAATACAAGGAGTCTGAAGCTTTATTCCTCAAGGCAATTAAAGCAAATCCAAA TGTTGCAAGTTACCATGGTAATTTGGCTGTGCTTTATCATCGCTGGGGACATCTTGACTCTGCCAAGAAA CACTATGAGATCTCCTTGCAGCTTGACCCTGTGGCAGTAGGCACCAAGGAGAATTACAGTCTTCTGAGAA GAAAGCTAGAACAAACGCAGAAGAAAGATGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208391 representing BC031368
Red=Cloning site Green=Tags(s) MLCKEQGITVLGLNAVFDILVIGKLDILAAVRKVLHKDKSQENAGMFKNGGLLFRIALLTIGGTSMLYIR WKIMGTGPPAFTEVDNPASFADSMLVRAINYNYYYSLNAWLLLCPWWLCFDWSMGCIPLIKSVGDWRVIA LAALWLCLIGLIFQALCSEDSCKRRILTLGLGFLVIPFLPASNLFFRVGFVVAERVLYLPSAGYCVLLTF GFGALSRHTKKKKPVAAIILGILLINALRCVIRSGEWRSEEQLFRSALSVCPLNAKVHYNIGKNLADQGN QTAAIKYYREAVRLNPKYVHAMNNLGNILKERNELQEAEELLSLAVQIQPDFAAAWMNLGIVQNSLKRFE EAEQSYRTAIKHRRKYPDCYYNLGRLYADLNRHVDALNAWRNATVLKPEHSLAWNNMIILLDNTGNLAQA EAVGREALQLIPNDHSLMFSLANVLGKSQKYKESEALFLKAIKANPNVASYHGNLAVLYHRWGHLDSAKK HYEISLQLDPVAVGTKENYSLLRRKLEQTQKKDV TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC031368 |
| ORF Size | 1572 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC031368, AAH31368 |
| RefSeq Size | 2824 bp |
| RefSeq ORF | 1574 bp |
| Locus ID | 70551 |
| Gene Summary | Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. The 4 members of the TMTC family are O-mannosyl-transferases dedicated primarily to the cadherin superfamily, each member seems to have a distinct role in decorating the cadherin domains with O-linked mannose glycans at specific regions. Also acts as O-mannosyl-transferase on other proteins such as PDIA3.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217872 | Tmtc4 (untagged) - Mouse transmembrane and tetratricopeptide repeat containing 4 (cDNA clone MGC:32342 IMAGE:5029461), (10ug) |
CNY 3912.00 |
|
| MR208391 | Tmtc4 (Myc-DDK-tagged) - Mouse transmembrane and tetratricopeptide repeat containing 4 (cDNA clone MGC:32342 IMAGE:5029461) |
CNY 3904.00 |
|
| MR208391L3 | Lenti ORF clone of Tmtc4 (Myc-DDK-tagged) - Mouse transmembrane and tetratricopeptide repeat containing 4 (cDNA clone MGC:32342 IMAGE:5029461) |
CNY 4850.00 |
|
| MR208391L4 | Lenti ORF clone of Tmtc4 (mGFP-tagged) - Mouse transmembrane and tetratricopeptide repeat containing 4 (cDNA clone MGC:32342 IMAGE:5029461) |
CNY 4850.00 |
