E4f1 (BC071228) Mouse Tagged ORF Clone
CAT＃: MG208562
- TrueORF®
E4f1 (tGFP-tagged) - Mouse E4F transcription factor 1 (cDNA clone MGC:78185 IMAGE:4915902)
ORF Plasmid: DDK
"BC071228" in other vectors (4)
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CNY 3330.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | p120E4F; phi-AP3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208562 representing BC071228
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGACGATGTGCACAGATGTGGCCGCTGCCAAGTGGAATTCACTGCCTTGGAGGACTTTGTCCAGCA CAAGATTCAGAAGACCTGCCATCGGGCCCCGCAGGAGGCCCTGCCCACCACCCCTTGCCACTGCACTGCT GGACCAGGAGGTGGTGCCCACAGCAGCAGAGGGAGGTCCAGATGAACCCATCACTGTGGCTCACATCGTG GTGGAGGCCACCTCTCTAGCAGAAGACATCAGCCACGCTCCTGACCTTGTTGGAAGTGGGCACATCAAAG AGGTCATTGTGGCTGCTGAGGCAGAGCCAGGGGATGGCGAGATGGCAGAGGCTCCAGGCAGTCCTAATCA TCAGGAACTTGGGCTTCTTGGGGAGGGCGAGCAGGCCCATGTCAAGCTGCTGGTGAACAAGGAAGGCCGC TACGTGTGCATGCTATGTCACAAGACCTTCAAAACGGGCAGCATCCTCAAGGCCCACATGGTAACGCACA GCAGCCGCAAGGACCACGAGTGCAAGCTCTGTGGGGCCTCTTTTCGGACCAAGGGCTCTCTCATCCGGCA CCACCGACGGCACACTGATGAGCGCCCCTACAAATGTGCCAAGTGTGGAAAGAGTTTCCGAGAGTCAGGC GCACTGACTCGGCACCTCAAATCTCTCACTCCATGCACAGAAAAGATCCGCTTCAGCATAAGCAAGGACA CAGCTGTGGGCAAAGAGGAAGTGCCTGCAGGGTCCAGTGCCTCCACTGTGGGGACGGTGACATCATCAGT GGCAGGAGACCCCATGGAGACATCACCTGTGATTCACCTGGTGACAGATGCCAAGGGTACTGTCATCCAT GAAGTCCACGTCCAGATGCAGGAGCTTCCCCTGGGCATGAAAGCCCTGACCCCAGAGTCCCCAGACTCGG AGGAGCTCCCCTGTTCCAGTGAGAACAGCCGTGAGAACCTGCTACATCAGGCCATGCAGAATTCTGGCAT CGTCCTTGAGAGGGTTGCTGGAGAGGAGAGTGCTCTGGAGCCAGCCCCTCCCTCTGGGTCCAGTCCCCAG TGCCTGGGAGATGGATCCCCTGAACTGCCTCTGCTGAAGGTGGAGCAGATAGAGACACAGGTGGCCAGTG AGGCCGCCACCGTGCCCAGGACCCACCCGTGCCCTCAGTGCAGTGAGACTTTCCCAACAGCAGCCACGCT GGAGGCCCACAAGAGAGGTCACATAGTGTGGCAAGGCCTTCCCCAAAGCCTACCTGCTCAAGAAGCACCA GGAGGTGCACGTGCACGAGCGCCGCTTCCGTTGTGGAGACTGTGGGAAGCTTTACAAGACCATCGCTCAT GTGCGGGGCCACCGGCGTGTTCACTCAGACGAGAGGCCTTTCCCTTGTCCCCAGTGCGGCAAGCGTTACA AAACCAAGAATGCCCAGCAAGTACACTTCCGGACACATCTGGAAGAAAAGCCCCACGTGTGCCAGTTCTG CAGCCGAGGCTTCCGGGAGAAGGGCTCTCTGGTGCGGCATGTGAGGCACCACACAGGCGAGAAACCTTTC AAGTGCTACAAGTGTGGCCGTGGCTTCGCGGAGCATGGCACACTCAACCGGCACCTGCGCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208562 representing BC071228
Red=Cloning site Green=Tags(s) MRTMCTDVAAAKWNSLPWRTLSSTRFRRPAIGPRRRPCPPPLATALLDQEVVPTAAEGGPDEPITVAHIV VEATSLAEDISHAPDLVGSGHIKEVIVAAEAEPGDGEMAEAPGSPNHQELGLLGEGEQAHVKLLVNKEGR YVCMLCHKTFKTGSILKAHMVTHSSRKDHECKLCGASFRTKGSLIRHHRRHTDERPYKCAKCGKSFRESG ALTRHLKSLTPCTEKIRFSISKDTAVGKEEVPAGSSASTVGTVTSSVAGDPMETSPVIHLVTDAKGTVIH EVHVQMQELPLGMKALTPESPDSEELPCSSENSRENLLHQAMQNSGIVLERVAGEESALEPAPPSGSSPQ CLGDGSPELPLLKVEQIETQVASEAATVPRTHPCPQCSETFPTAATLEAHKRGHIVWQGLPQSLPAQEAP GGARARAPLPLWRLWEALQDHRSCAGPPACSLRREAFPLSPVRQALQNQECPASTLPDTSGRKAPRVPVL QPRLPGEGLSGAACEAPHRRETFQVLQVWPWLRGAWHTQPAPAH TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC071228 |
| ORF Size | 1602 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC071228, AAH71228 |
| RefSeq Size | 2551 bp |
| RefSeq ORF | 1604 bp |
| Locus ID | 13560 |
| Gene Summary | This gene encodes a member of the GLI-Kruppel zinc finger family. The encoded protein is likely to be multi-functional, with both adenovirus E1A-regulated transcription factor and ubiquitin E3 ligase activities, including roles in cell cycle regulation and the ubiquitination of p53. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217697 | E4f1 (untagged) - Mouse E4F transcription factor 1 (cDNA clone MGC:78185 IMAGE:4915902), (10ug) |
CNY 6940.00 |
|
| MR208562 | E4f1 (Myc-DDK-tagged) - Mouse E4F transcription factor 1 (cDNA clone MGC:78185 IMAGE:4915902) |
CNY 3976.00 |
|
| MR208562L3 | Lenti ORF clone of E4f1 (Myc-DDK-tagged) - Mouse E4F transcription factor 1 (cDNA clone MGC:78185 IMAGE:4915902) |
CNY 4940.00 |
|
| MR208562L4 | Lenti ORF clone of E4f1 (mGFP-tagged) - Mouse E4F transcription factor 1 (cDNA clone MGC:78185 IMAGE:4915902) |
CNY 4940.00 |
