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Nprl3 (NM_181569) Mouse Tagged ORF Clone
CAT＃: MG208964
- TrueORF®
Nprl3 (tGFP-tagged) - Mouse alpha globin regulatory element containing gene (Mare)
ORF Plasmid: DDK
"NM_181569" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Aag; CGTHBA; HS-26; HS-40; m(alpha)RE; Mare; Phg; Prox1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208964 representing NM_181569
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGACAATACCAGCCCCATCAGCGTGATCCTGGTGAGCTCGGGCAGCAGGGGCAATAAGCTGCTCT TCAGGTACCCCTTCCAGAGGAGCCAGGAGCACCCCGCGTCCCAGACAAATAAACCACGGAGCAGATATGC TGTCAACAACACTGGCGAACATGCTGATGACCAGGACGGTGACTCCAGGTTCTCGGATGTTATTCTGGCA ACAATTTTGGCAACCAAATCTGAAATGTGCGGCCAAAAATTTGAACTGAAGATTGATAATGTACGGTTTG TTGGGCACCCAACACTGCTGCAGCATGCTCTGGGGCAGGTCTCCAAAACAGATCCATCTCCAAAGCGGGA AGCTCCGACCATGATTCTTTTTAACGTGGTATTTGCACTGAGGGCCAATGCTGATCCATCAGTGATAAAC TGTCTACACAACTTGTCCCGCCGAATTGCTACTGTCCTGCAGCATGAGGAGCGCCGCTGCCAGTACCTCA CTCGAGAGGCCAAGCTGATCCTAGCACTTCAGGATGAGGTGTCTGCTATGGCTGATGCAAATGAAGGTCC TCAGTCCCCATTCCAGCACATCCTACCCAAGTGCAAGCTGGCTCGGGACCTCAAAGAAGCTTATGACAGC CTGTGCACATCTGGTGTGGTACGGCTTCACATTAACAGCTGGCTGGAAGTGAGTTTCTGTCTGCCCCACA AGATCCACTATGCAGCTTCAAGTCTGATTCCTCCTGAGGCTATTGAACGGAGCCTGAAAGCCATCCGCCC GTACCATGCCTTGCTACTTCTCAGTGACGAGAAGTCTCTGCTGAGTGAGCTTCCCATTGACTGCTCCCCG GCTCTGGTGCGGGTGATCAAGACCACGTCTGCTGTGAAGAACCTGCAGCAGCTAGCCCAGGATGCCGATC TGGCCTTGCTGCAGGTCTTCCAGTTGGCAGCTCACCTGGTATACTGGGGCAAGGCTGTCATCATCTACCC ACTGTGTGAGAACAACGTCTATGTCATGTCTCCCAATGCCAGTGTGTGTCTGTACTCCCCGCTAGCTGAA CAGTTCTCCCGGCAGTTCCCGTCCCATGACCTGCCATCTGTCCTGGCCAAGTTTTCCTTGCCTGTTTCTT TATCGGAGTTCAGGAGCCCTCTGGCTCCCCCTGCACAGGAGACCCAGCTCATCCAGATGGTGGTGTGGAT GCTGCAGCGCCGGCTCCTCATCCAGCTGCATACCTATGTTTGCCTAATGGCCTCACCCAGCGAAGAGGAG CCCCGACTGCGAGAGGATGATGTCCCCTTCACAGCCAGAGTTGGTGGCCGCAGCCTCAGCACACCCAATG CTCTAAGCTTTGGCTCCCCAACCAGCAGTGACGACATGACCCTTACCAGCCCCAGTATGGACAACTCAAG TGCTGAGCTGCTCCCCAGTGGGGACTCACCACTGAACAAGAGGATGACAGAGAACCTGCTGGCTAGCCTC TCAGAGCATGAGCGGGCTGCTATCCTCAATGTGCCTGCAGCCCAAAACCCTGAGGACCTCCGCATGTTTG CCAGGCTCCTTCACTACTTCCGTGGCCGCCACCATCTGGAGGAGATCATGTACAATGAGAACACTCGGCG CTCCCAGCTACTCATGCTCTTTGACAAATTCCGCAGTGTGCTGGTGGTGACCACCCATGAGGACCCTGTT ATTGCCGTCTTCCAGGCACTGCTCACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208964 representing NM_181569
Red=Cloning site Green=Tags(s) MGDNTSPISVILVSSGSRGNKLLFRYPFQRSQEHPASQTNKPRSRYAVNNTGEHADDQDGDSRFSDVILA TILATKSEMCGQKFELKIDNVRFVGHPTLLQHALGQVSKTDPSPKREAPTMILFNVVFALRANADPSVIN CLHNLSRRIATVLQHEERRCQYLTREAKLILALQDEVSAMADANEGPQSPFQHILPKCKLARDLKEAYDS LCTSGVVRLHINSWLEVSFCLPHKIHYAASSLIPPEAIERSLKAIRPYHALLLLSDEKSLLSELPIDCSP ALVRVIKTTSAVKNLQQLAQDADLALLQVFQLAAHLVYWGKAVIIYPLCENNVYVMSPNASVCLYSPLAE QFSRQFPSHDLPSVLAKFSLPVSLSEFRSPLAPPAQETQLIQMVVWMLQRRLLIQLHTYVCLMASPSEEE PRLREDDVPFTARVGGRSLSTPNALSFGSPTSSDDMTLTSPSMDNSSAELLPSGDSPLNKRMTENLLASL SEHERAAILNVPAAQNPEDLRMFARLLHYFRGRHHLEEIMYNENTRRSQLLMLFDKFRSVLVVTTHEDPV IAVFQALLT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_181569 |
| ORF Size | 1707 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_181569.3, NP_853547.1 |
| RefSeq Size | 2316 bp |
| RefSeq ORF | 1710 bp |
| Locus ID | 17168 |
| UniProt ID | Q8VIJ8 |
| Gene Summary | As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204836 | Nprl3 (untagged) - Mouse nitrogen permease regulator-like 3 (S. cerevisiae) (Nprl3), (10ug) |
CNY 2,000.00 |
|
| MR208964 | Nprl3 (Myc-DDK-tagged) - Mouse nitrogen permease regulator-like 3 (S. cerevisiae) (Nprl3) |
CNY 4,240.00 |
|
| MR208964L3 | Lenti ORF clone of Nprl3 (Myc-DDK-tagged) - Mouse nitrogen permease regulator-like 3 (S. cerevisiae) (Nprl3) |
CNY 6,460.00 |
|
| MR208964L4 | Lenti ORF clone of Nprl3 (mGFP-tagged) - Mouse nitrogen permease regulator-like 3 (S. cerevisiae) (Nprl3) |
CNY 6,460.00 |
