Tsr1 (BC031531) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	mKIAA1401, RP23-174M12.4
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209042 representing BC031531 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAAAGTCGCTGATACCATCCTGTTCCTCCTGGACCCACTAGAAGGCTGGGACAGTACGGGGGATT ATTGCCTGTCTTGCCTTTTTGCTCAGGGCCTTCCCACCTATACACTAGCTGTCCAGGGCCTTTCTGGCTT CCCACCAAAGAAACAAATAGATGCCCGAAAGAAGCTAAGTAAAACGGTGGAGAAACGCTTTCCTGAAGAC AAACTTCTGCTCCTAGACACGCAGCAGGAATCAGGAATGCTACTTAGACAGTTGGCTAACCAGAAGCAAC GGCATCTTGCTTTTCGGGATCGGCGAGCTTACTTGTTTGCCCATGTTGCTGACTTCGTGCCTAGTGAGGA GAGTGACTTAGTGGGCACCTTGAAAATCTCAGGCTATGTTCGTGGGAGGACTCTGAATGTAAATAGCTTG CTGCATATCGTTGGACACGGTGATTTCCAAATGAATCAAATAGATGCTCCTGTGGATCCGTTCCCTTTAA ATCCTAGAGTGATTAAATCCCAGAAGAAACCAAACATGGCAATGGAGGTTTGTGTTACAGATGCTGCTGC TGACATGGAAGAAGACCTTAAGGTTCTGATGAAGGCAGACCCTGATCACCAGGAGTCTTTACAAACAGAG GCTATTCCAGATCCTATGGAAGGAGAGCAGACCTGGCCCACTGAGGAAGAGCTGGATGAGGCAGATGACT TACTGAAGCAACGGTCCAGAGTGGTAAAGAAGGTCCCTAAAGGAACATCCAGTTACCAGGCTGAGTGGAT TCTGGATGAAGGTGATGAAAGTGATGGGGAAGGTGGTGAATATGATGATATACAACACGAAGGTTTCATG GAAGAGGAGTCTCAGGATGGGAGTGGTGAGGAAGAGGAGGAAGAATGTGAAACTATGACTCTTGGGGAGT CTGTGCGTGACGATCTATACGATGAGAAAGTAGATGAAGAGGACGAGGAGAGAATGTTGGAGAAATATAA ACAAGAAAGACTGGAAGAGATGTTTCCAGATGAAATGGATACCCCCCGTGATGTAGCTGCTAGAATTCGA TTTCAGAAATATAGAGGCCTCAAGAGTTTCCGGACATCTCCTTGGGATCCTAAAGAAAACCTTCCTAGAG ATTATGCTCGGATATTTCAGTTTCAGAATTTTGTTAATACTAGAAAAAGAATTTTTAAAGAGATTGAGGA AAAAGAAGCTGAAGGAGCTGAGGTGGGCTGGTATGTCACACTCCATGTTTCTGATGTCCCTGTCTCAGTG GTTGAGTATTTCAGGCAAGGAGCGCCCTTGATTGCATTTTCTTTGCTACCTTATGAACAGAAGATGTCCG TATTGAACATGGTGGTGAGCCGGAACCCTGGCAACACGGAACCTGTGAAGGCGAAAGAAGAATTGATCTT CCACTGTGGGTTCAGGCGCTTCCGAGCTTCGCCTTTGTTCTCTCAGCACACAGCAGCTGATAAACATAAA TTTCAAAGATTCCTGACTGCTGATGCTGCCTTAGTGGTGACAGTGTTTGCGCCAATCACGTTCCCTCCTG CATCTGTGCTGCTTTTCAAGCAGAGAAGAAATGGAATGCACAGCCTCATCGCTACAGGCCATCTGTTTTC AGTGGACCCAGACAGGATGGTCATAAAGAGAGTGGTTCTAAGTGGTCATCCTTTTAAAATATTTACTAAG ATGGCAGTAGTGCGGTACATGTTCTTCAACAGAGGATGTGATGTGGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209042 representing BC031531 Red=Cloning site Green=Tags(s) MAKVADTILFLLDPLEGWDSTGDYCLSCLFAQGLPTYTLAVQGLSGFPPKKQIDARKKLSKTVEKRFPED KLLLLDTQQESGMLLRQLANQKQRHLAFRDRRAYLFAHVADFVPSEESDLVGTLKISGYVRGRTLNVNSL LHIVGHGDFQMNQIDAPVDPFPLNPRVIKSQKKPNMAMEVCVTDAAADMEEDLKVLMKADPDHQESLQTE AIPDPMEGEQTWPTEEELDEADDLLKQRSRVVKKVPKGTSSYQAEWILDEGDESDGEGGEYDDIQHEGFM EEESQDGSGEEEEEECETMTLGESVRDDLYDEKVDEEDEERMLEKYKQERLEEMFPDEMDTPRDVAARIR FQKYRGLKSFRTSPWDPKENLPRDYARIFQFQNFVNTRKRIFKEIEEKEAEGAEVGWYVTLHVSDVPVSV VEYFRQGAPLIAFSLLPYEQKMSVLNMVVSRNPGNTEPVKAKEELIFHCGFRRFRASPLFSQHTAADKHK FQRFLTADAALVVTVFAPITFPPASVLLFKQRRNGMHSLIATGHLFSVDPDRMVIKRVVLSGHPFKIFTK MAVVRYMFFNRGCDVV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC031531
ORF Size	1728 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC031531, AAH31531
RefSeq Size	3379 bp
RefSeq ORF	1730 bp
Locus ID	104662
Gene Summary	Required during maturation of the 40S ribosomal subunit in the nucleolus.[UniProtKB/Swiss-Prot Function]

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