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Cul4a (NM_146207) Mouse Tagged ORF Clone
CAT＃: MG209429
- TrueORF®
Cul4a (tGFP-tagged) - Mouse cullin 4A (Cul4a)
ORF Plasmid: DDK
"NM_146207" in other vectors (4)
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CNY 9,936.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2810470J21Rik; AW495282 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209429 representing NM_146207
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGAGGGCCCTCGGAAGGGCAGCGTCTCGGCTCTCATGGGCCGCACCAACGGCCTCACCAAGC CCGCGGCGTTGGCCGGTGGCCCCGCCAAGCCGGGGGGCACGGGAGGCTCCAGGAAGCTGGTCATCAAGAA CTTCCGAGACAGGCCGCGGCTGCCTGACAACTACACTCAGGACACGTGGCGGAAGCTTCACGAAGCGGTC AAGGCCATCCAGAGCAGCACATCCATCAGGTACAACCTGGAGGAGCTGTACCAGGCTGTTGAAAATCTTT GTTCTCACAAAGTCTCCCCAACGCTCTACAAGCAGCTGCGCCAGGTCTGTGAAGACCATGTCCAGGCCCA GATCCTCCCATTCAGAGAAGACTCACTAGACAGCGTTTTATTTCTAAAGAAGATTAACACATGCTGGCAA GATCACTGCAGACAAATGATCATGATCAGAAGCATCTTCCTGTTTCTGGATCGAACCTACGTCCTCCAGA ATTCCATGCTGCCTTCTATCTGGGACATGGGCCTAGAGCTGTTTAGGAACCACATCATCAGCGACAGGAT GGTGCAGAGTAAGACCATTGATGGGATCCTGCTGCTGATTGGGCGTGAACGAAGTGGAGAGGCTGTGGAC AGGAGTCTGCTTCGGAGTTTGCTAAGCATGCTCTCAGATCTCCAGGTGTACAAAGACTCATTTGAGCTGA AGTTCTTGGAAGAGACTAACTGTCTATACGCTGCTGAAGGCCAGAGGCTGATGCAGGACAGGGAGGTTCC AGAATACCTTAACCATGTGAGCAAACGTCTAGAAGAGGAAGCAGACCGAGTGATCACCTACTTAGACCAC AGCACACAAAAACCGTTGATTGCCTGTGTGGAGAAACAGCTGTTAGGAGAACATTTGACAGCAATTCTAC AGAAAGGCCTAGAGCACCTGCTGGATGAGAACAGGGTGCCCGACCTCACCCAGATGTACCAGCTCTTCAG CCGGGTGAAAGGAGGGCAGCATGCGCTGCTGCAGCACTGGAGCGAGTACATCAAGACCTTTGGAACAACC ATTGTCATCAATCCTGAGAAAGACAAAGACATGGTCCAAGACCTGCTGGACTTTAAGGACAAAGTGGACC ACGTGGTGGAGGTGTGCTTCCAGAGGAACGAGCGCTTCATCAACCTGATGAAGGAGTCCTTCGAGACGTT CATCAACAAGAGACCAAACAAGCCTGCAGAGCTCATTGCCAAGCACGTGGACTCAAAGTTACGGGCCGGC AACAAAGAAGCCACAGATGAGGAACTGGAGAGGATCCTGGACAAGATCATGATTCTCTTCCGTTTCATCC ATGGTAAAGATGTCTTTGAAGCGTTTTATAAGAAAGACTTAGCAAAAAGGCTGCTGGTGGGGAAGAGCGC GTCGGTGGACGCGGAGAAGTCCATGCTGTCGAAGCTCAAGCACGAGTGCGGAGCTGCGTTTACCAGCAAA CTGGAGGGCATGTTCAAGGACATGGAGCTCTCCAAAGACATCATGGTTCACTTCAAGCAGCATATGCAGA ACCAGAGTGCGCCAGGCCCCATTGACCTCACGGTGAACATACTCACCATGGGCTACTGGCCCACGTACAC ACCCATGGAAGTGCACCTCCCTCCAGAGATGGTCAGACTTCAGGAAGTATTCAAGACGTTCTACCTGGGC AAGCACAGTGGGCGGAAGCTGCAATGGCAGACAACGCTGGGACACGCCGTGCTGAAGGCGGACTTTAAGG AGGGGAAGAAGGAGTTCCAGGTATCCCTCTTCCAGACGCTGGTGCTGCTCATGTTCAACGAAGGGGATGG ATTTAGCTTTGAAGAAATAAAAATGGCCACTGGCATAGAGGACAGTGAGTTGCGAAGAACACTACAGTCT CTGGCCTGTGGTAAAGCACGGGTCCTAATTAAAAGTCCCAAAGGAAAGGAGGTGGAAGATGGAGATAAGT TCATCTTCAATGCAGACTTCAAACACAAGTTGTTCAGAATAAAGATCAATCAAATCCAGATGAAGGAGAC CGTTGAAGAACAGGTTAGCACTACAGAGAGAGTATTCCAGGATAGACAGTACCAGATCGACGCTGCTATA GTCAGAATAATGAAAATGAGGAAGACACTTGGTCATAATCTTCTAGTTTCTGAATTATACAATCAGCTGA AATTTCCAGTGAAGCCTGGAGATTTGAAAAAAAGGATTGAGTCACTTATAGACAGAGACTATATGGAACG AGACAAAGACAGTCCAAATCAGTACCACTACGTGGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209429 representing NM_146207
Red=Cloning site Green=Tags(s) MADEGPRKGSVSALMGRTNGLTKPAALAGGPAKPGGTGGSRKLVIKNFRDRPRLPDNYTQDTWRKLHEAV KAIQSSTSIRYNLEELYQAVENLCSHKVSPTLYKQLRQVCEDHVQAQILPFREDSLDSVLFLKKINTCWQ DHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRNHIISDRMVQSKTIDGILLLIGRERSGEAVD RSLLRSLLSMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQDREVPEYLNHVSKRLEEEADRVITYLDH STQKPLIACVEKQLLGEHLTAILQKGLEHLLDENRVPDLTQMYQLFSRVKGGQHALLQHWSEYIKTFGTT IVINPEKDKDMVQDLLDFKDKVDHVVEVCFQRNERFINLMKESFETFINKRPNKPAELIAKHVDSKLRAG NKEATDEELERILDKIMILFRFIHGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSK LEGMFKDMELSKDIMVHFKQHMQNQSAPGPIDLTVNILTMGYWPTYTPMEVHLPPEMVRLQEVFKTFYLG KHSGRKLQWQTTLGHAVLKADFKEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQS LACGKARVLIKSPKGKEVEDGDKFIFNADFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAI VRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDSPNQYHYVA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_146207 |
| ORF Size | 2277 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_146207.3 |
| RefSeq Size | 3628 bp |
| RefSeq ORF | 2280 bp |
| Locus ID | 99375 |
| UniProt ID | Q3TCH7 |
| Gene Summary | Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX(DTL) directs autoubiquitination of DTL (By similarity). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (By similarity). With CUL4B, contributes to ribosome biogenesis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221394 | Cul4a (untagged) - Mouse cullin 4A (Cul4a), (10ug) |
CNY 9,790.00 |
|
| MR209429 | Cul4a (Myc-DDK-tagged) - Mouse cullin 4A (Cul4a) |
CNY 8,336.00 |
|
| MR209429L3 | Lenti ORF clone of Cul4a (Myc-DDK-tagged) - Mouse cullin 4A (Cul4a) |
CNY 8,550.00 |
|
| MR209429L4 | Lenti ORF clone of Cul4a (mGFP-tagged) - Mouse cullin 4A (Cul4a) |
CNY 8,550.00 |
