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Pabpc1 (NM_008774) Mouse Tagged ORF Clone
CAT＃: MG209653
- TrueORF®
Pabpc1 (tGFP-tagged) - Mouse poly A binding protein, cytoplasmic 1 (Pabpc1)
ORF Plasmid: DDK
"NM_008774" in other vectors (5)
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CNY 7,030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ePAB; PABP; Pabp1; PabpI; Pabpl1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209653 representing NM_008774
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCCCAGCGCCCCCAGCTACCCCATGGCCTCTCTGTACGTGGGGGACCTGCACCCCGACGTGACCG AGGCGATGCTCTACGAGAAGTTCAGCCCGGCCGGGCCCATCCTCTCCATCCGGGTCTGCAGGGACATGAT CACCCGCCGCTCCTTGGGCTACGCGTACGTGAACTTCCAGCAGCCGGCGGACGCGGAACGTGCTTTGGAC ACCATGAATTTTGATGTTATAAAGGGCAAGCCAGTACGCATCATGTGGTCTCAGCGTGATCCATCACTTC GCAAAAGTGGAGTAGGCAACATATTCATTAAAAATTTGGACAAATCCATCGACAATAAAGCACTATATGA TACGTTTTCTGCGTTTGGTAACATCCTTTCATGTAAGGTGGTTTGTGATGAAAATGGCTCCAAGGGCTAT GGATTTGTACACTTTGAAACACAGGAAGCAGCTGAAAGAGCTATTGAAAAAATGAATGGGATGCTTCTAA ATGATCGTAAAGTGTTTGTTGGACGATTTAAATCTCGGAAGGAACGAGAAGCAGAGCTTGGAGCCAGGGC AAAGGAGTTCACCAATGTTTACATCAAGAACTTCGGAGAAGACATGGATGATGAGCGCCTTAAGGAACTC TTTGGCAAGTTTGGGCCTGCCTTAAGTGTGAAAGTAATGACAGATGAAAGTGGAAAATCCAAAGGATTTG GATTTGTAAGCTTTGAAAGGCATGAAGATGCGCAGAAAGCTGTGGATGAGATGAATGGGAAGGAGCTCAA TGGAAAACAGATTTATGTTGGACGAGCTCAGAAAAAAGTGGAACGGCAGACGGAACTTAAGCGCAAATTT GAGCAGATGAAGCAAGATAGGATCACCAGATATCAGGGTGTGAACCTTTATGTGAAAAATCTTGATGACG GGATTGATGATGAGCGTCTCCGGAAGGAGTTTTCTCCGTTTGGTACAATCACCAGTGCAAAAGTAATGAT GGAGGGTGGGCGCAGCAAAGGGTTTGGTTTTGTATGTTTCTCATCCCCTGAAGAAGCCACTAAAGCAGTT ACAGAGATGAATGGTAGAATTGTGGCCACGAAGCCACTGTATGTAGCTTTAGCTCAGCGCAAAGAAGAGC GCCAGGCTCACCTCACTAACCAGTATATGCAGAGGATGGCAAGTGTACGAGCTGTGCCCAACCCCGTGAT CAACCCCTACCAGCCAGCACCTCCTTCAGGTTACTTCATGGCAGCTATCCCACAGACTCAGAACCGTGCT GCATACTATCCTCCTAGCCAAATTGCTCAACTAAGACCAAGTCCTCGCTGGACTGCTCAGGGTGCCAGAC CTCATCCATTCCAGAATATGCCCGGTGCTATCCGCCCAGCTGCTCCTAGACCACCATTTAGTACGATGAG ACCAGCTTCCTCACAGGTTCCACGAGTCATGTCAACACAGCGTGTTGCTAACACATCAACACAGACAATG GGTCCACGTCCTGCAGCTGCTGCTGCTGCAGCCACTCCTGCTGTCCGCACCGTTCCCCAGTATAAATATG CTGCGGGAGTCCGCAATCCCCAGCAACATCTTAATGCACAGCCACAAGTTACCATGCAACAGCCTGCTGT TCATGTGCAAGGTCAAGAACCTTTAACTGCTTCCATGTTGGCATCTGCGCCCCCGCAAGAGCAGAAGCAA ATGTTGGGTGAACGGCTGTTTCCTCTTATCCAAGCCATGCACCCTTCTCTTGCTGGTAAAATCACTGGCA TGCTGTTGGAGATTGATAACTCAGAATTACTTCACATGCTCGAGTCTCCAGAGTCTCTCCGCTCAAAGGT TGATGAAGCTGTAGCTGTACTACAAGCCCACCAAGCGAAAGAGGCTGCCCAGAAAGCAGTGAACAGTGCC ACTGGTGTTCCAACTGTC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209653 representing NM_008774
Red=Cloning site Green=Tags(s) MNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERALD TMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGY GFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKEL FGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKF EQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAV TEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQPAPPSGYFMAAIPQTQNRA AYYPPSQIAQLRPSPRWTAQGARPHPFQNMPGAIRPAAPRPPFSTMRPASSQVPRVMSTQRVANTSTQTM GPRPAAAAAAATPAVRTVPQYKYAAGVRNPQQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQ MLGERLFPLIQAMHPSLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSA TGVPTV SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008774 |
| ORF Size | 1908 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008774.2, NP_032800.2 |
| RefSeq Size | 2834 bp |
| RefSeq ORF | 1911 bp |
| Locus ID | 18458 |
| UniProt ID | P29341 |
| Gene Summary | Binds the poly(A) tail of mRNA, including that of its own transcript. May be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed (By similarity). By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR209653 | Pabpc1 (Myc-DDK-tagged) - Mouse poly(A) binding protein, cytoplasmic 1 (Pabpc1) |
CNY 7,112.00 |
|
| MR209653L1 | Lenti ORF clone of Pabpc1 (Myc-DDK-tagged) - Mouse poly(A) binding protein, cytoplasmic 1 (Pabpc1) |
CNY 9,512.00 |
|
| MR209653L2 | Lenti ORF clone of Pabpc1 (mGFP-tagged) - Mouse poly(A) binding protein, cytoplasmic 1 (Pabpc1) |
CNY 8,270.00 |
|
| MR209653L3 | Lenti ORF clone of Pabpc1 (Myc-DDK-tagged) - Mouse poly(A) binding protein, cytoplasmic 1 (Pabpc1) |
CNY 8,270.00 |
|
| MR209653L4 | Lenti ORF clone of Pabpc1 (mGFP-tagged) - Mouse poly(A) binding protein, cytoplasmic 1 (Pabpc1) |
CNY 8,270.00 |
