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Grk2 (NM_130863) Mouse Tagged ORF Clone
CAT＃: MG209736
- TrueORF®
Adrbk1 (tGFP-tagged) - Mouse adrenergic receptor kinase, beta 1 (Adrbk1)
ORF Plasmid: DDK
"NM_130863" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Adrbk-1; Bark-1; beta ARK; betaARK1; GRK2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209736 representing NM_130863
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAAGTATCTGGAGGACCGAGGAGAAGTGACCTTTGAAAAGATCTTTTCGCAGAAGTTAGGGTACC TGCTTTTCCGGGACTTCTGCCTGAACCATCTGGAAGAGGCCAAGCCCTTGGTGGAGTTCTACGAAGAGAT CAAGAAATATGAGAAGCTGGAGACAGAGGAGGAACGTGTGGTCCGCAGCCGAGAGATCTTTGACTCCTAT ATTATGAAAGAACTGCTGGCCTGCTCACATCCCTTTTCAAAGAATGCTACGGAGCATGTCCAGGGCCACC TGGTGAAGAAGCAGGTGCCTCCAGATCTCTTCCAGCCATACATTGAGGAGATTTGTCAGAACCTCCGAGG GGACGTGTTCCAGAAGTTCATAGAGAGTGACAAGTTCACACGGTTCTGCCAGTGGAAGAATGTTGAGCTC AACATCCACCTGACCATGAATGACTTCAGTGTGCATCGAATCATCGGGCGTGGGGGCTTCGGTGAGGTCT ATGGGTGCCGGAAAGCAGACACAGGCAAGATGTACGCCATGAAGTGTCTGGACAAGAAACGCATCAAGAT GAAGCAGGGAGAGACCCTGGCTCTGAACGAGCGGATCATGCTTTCCCTCGTCAGCACCGGGGACTGCCCC TTCATCGTGTGCATGTCATATGCATTCCACACACCAGACAAGCTCAGCTTCATCCTGGACCTCATGAACG GTGGGGACCTGCACTACCACCTGTCTCAGCATGGAGTCTTCTCTGAAGCCGACATGCGCTTCTACGCCGC TGAGATCATCCTGGGCCTTGAACACATGCACAATCGCTTTGTAGTCTACAGGGACCTGAAGCCAGCCAAC ATTCTCCTGGATGAACATGGCCATGTGAGAATCTCGGACCTGGGCCTAGCCTGTGACTTCTCCAAGAAGA GGCCTCATGCCAGTGTGGGCACACACGGGTACATGGCCCCCGAAGTCCTACAGAAGGGCGTGGCCTATGA CAGCAGTGCAGACTGGTTCTCCCTGGGCTGCATGCTCTTCAAGTTGTTGCGGGGACACAGCCCCTTCCGG CAGCACAAGACCAAAGACAAGCATGAGATTGACCGCATGACGTTGACGATGGCTGTAGAGCTGCCTGACT CCTTCTCCCCTGAACTCCGATCCCTGCTGGAAGGTTTGTTGCAGAGGGACGTCAATCGGAGACTAGGCTG TCTGGGCCGTGGGGCTCAGGAGGTAAAAGAAAGTCCTTTCTTTCGTTCCCTGGACTGGCAGATGGTCTTC TTACAGAAGTACCCTCCTCCACTGATCCCCCCACGTGGGGAGGTGAATGCAGCTGACGCTTTCGACATTG GCTCCTTTGATGAGGAGGACACAAAAGGAATCAAGTTACTGGACAGTGACCAGGAACTGTACCGCAACTT CCCTCTCACCATCTCTGAGCGGTGGCAACAGGAGGTGGCAGAGACTGTCTTTGATACCATCAATGCTGAA ACAGACCGGCTGGAGGCACGAAAGAAGGCCAAAAACAAACAGCTGGGCCACGAGGAGGACTATGCCCTGG GTAAGGACTGCATCGTGCATGGCTACATGTCCAAGATGGGCAACCCCTTCCTGACCCAGTGGCAGCGGCG ATACTTCTACTTGTTCCCCAACCGGCTCGAGTGGAGGGGTGAAGGCGAGGCTCCGCAGAGCCTCCTGACC ATGGAGGAGATCCAGTCAGTGGAAGAGACACAGATCAAGGAACGCAAGTGTCTCCTGCTTAAGATCCGAG GTGGCAAGCAGTTTGTCCTGCAGTGTGATAGTGATCCAGAGCTGGTGCAATGGAAAAAGGAGCTGCGTGA TGCCTACCGCGAGGCCCAGCAGCTGGTACAGCGAGTACCCAAGATGAAGAACAAGCCACGCTCGCCTGTG GTGGAGCTGAGCAAGGTGCCGCTGATCCAGCGTGGCAGTGCCAACGGCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209736 representing NM_130863
Red=Cloning site Green=Tags(s) MQKYLEDRGEVTFEKIFSQKLGYLLFRDFCLNHLEEAKPLVEFYEEIKKYEKLETEEERVVRSREIFDSY IMKELLACSHPFSKNATEHVQGHLVKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNVEL NIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCP FIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPAN ILLDEHGHVRISDLGLACDFSKKRPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFR QHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVF LQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAE TDRLEARKKAKNKQLGHEEDYALGKDCIVHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLT MEEIQSVEETQIKERKCLLLKIRGGKQFVLQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPV VELSKVPLIQRGSANGL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_130863 |
| ORF Size | 1941 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_130863.2 |
| RefSeq Size | 3332 bp |
| RefSeq ORF | 1944 bp |
| Locus ID | 110355 |
| Gene Summary | Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by faciltating the trafficking of SMO into the cilium and the stimulation of SMO activity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204540 | Adrbk1 (untagged) - Mouse adrenergic receptor kinase, beta 1 (Adrbk1), (10ug) |
CNY 2,000.00 |
|
| MR209736 | Adrbk1 (Myc-DDK-tagged) - Mouse adrenergic receptor kinase, beta 1 (Adrbk1) |
CNY 4,560.00 |
|
| MR209736L3 | Lenti ORF clone of Adrbk1 (Myc-DDK-tagged) - Mouse adrenergic receptor kinase, beta 1 (Adrbk1) |
CNY 6,460.00 |
|
| MR209736L4 | Lenti ORF clone of Adrbk1 (mGFP-tagged) - Mouse adrenergic receptor kinase, beta 1 (Adrbk1) |
CNY 6,460.00 |
