Gas2l1 (BC031785) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	TU-71.1, GAR22, RP23-64E17.7
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209974 representing BC031785 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAACCCAGTGGCGGGCATCGCAGGCTCTGCCGCCAAGAGTGTGCGACCCTTCCGATCCAGTGAGG CCTATGTGGAAGCCATGAAGGAAGACCTGGCCGATTGGCTCAATGCTTTGTACAGTCTAGGTCTCCCCGG CAGTGGCGATGGCTTCCTCACGGGGCTGGCCACAGGCACAACCCTGTGCCAACATGCCAACGCTGTCACC GAGGCTGCCCGGGCTCTGGCTGCTGCCCGTCCAACCCGAGGTGTGGCCTTCCAGGCACACAGTGTGGCGC CTGGCTCCTTCATGGCCCGAGACAACGTGGCCTCTTTCATCGGCTGGTGCCGGGCTGAGCTGGGCGTGCC TGAAGTGCTCATGTTTGAGACAGAGGACCTGGTGCTACGCAAGAACGAGAAGAGTGTGGTGCTCTGCCTG CTGGAGGTCGCCCGGCGTGGGGCGCGCCTTGGCCTGCTTGCCCCGAGGCTGGTACAGTTCGAGCAGGAGA TCGAGCGGGAGCTTCGAGCTACCCCTCAGGTTTCCAGCGTCCCTGCTGCTGAAGAGGATGTCACTGAAAT TGCCACTGTACCAGGGGTTCCCACCCGCACACCCCGCATGACGCCCAATGACCTTCGAAACCTTGATGAG CTGGTAAGGGAGATCCTAGGCAGGTGCACCTGCCCCGACCAGTTTCCCATGATCAAGGTCTCCGAGGGGA AGTACCGAGTGGGAGACTCCAGCTTGCTCATCTTCGTGAGGGTGCTGAGGAGTCACGTGATGGTGCGAGT GGGGGGCGGCTGGGACACACTGGAGCACTACCTAGACAAGCATGACCCTTGTCGCTGCTCGTCTTCTACG CACCGTCTGCCCCAGCAGAGGACCGGGACTTTCTCCCCGCAGAGGGGATCACCCACTCCTAGCCCCCGCC CCGGTAGCCCAGTCCCTGGGAGTGAGCGCAGGAGCTCCCGGCCCGAAGTAACCCCCATCAGCCTGCGAGG CACAAAGGAGGGGCCTGAGACCCCGCTCAGGCCCCGCGATCAGCTGCCCCCCCTTCCCCGCTCCCGCCGC TACTCTGGGGACAGTGACTCCTCAGCCTCCTCTGCTCAGAGCGGCCCCATGGGTGCCCGCAGCGACGATT CAGCCACTGGCTCCCGGAGGGAGCGGCCCAGCCACCGGCCGACCTCCTGTCTTCCGGCCTCCCCGAGACG GCCGACCGCGCCTCGCAGCCAGTCTCGAGACCGGCTGGACCGGGGACGGCCCCGCGTGGCCCCAGGAGGC CGAGGCGCTCAGCTGTCGACCTCCAGCCCAGCAAGGCGCACCCGCAGCCAGAGCCGCGAGGAACAGGCGG TACTGATGGTGCGCAGGGACCGAGACGGCCAGCACTCGTGGGTGGCCCGGGGCAGGGGTGGCGGGGGATC AGGAGGTTCTGGGAGGAGTACCCCGCAGACTCCGCGTGCCCTCAGCCCTGCAGCACCCCGGCCTTCCAGG GGCCCCAGCCCCGGCCCAGAGCTGGCTGCCACACCTGCCAGCATCTTCCGCACGCCCCTGCAGCTTGACC CACAGCAGGAGCAGCAGCTGTTCAGGCGCCTGGAAGAGGAATTCTTAGCCAATGCCCGCGCCTTGGAGGC TGCTGCCAGCCATACCCCCATGGGATCTGCCCCTGACCCTCCAGCTCCGGACTCTGCTTACTGTTCATCC AGCTCCTCGTCTTCATCACTCAGTGTCCTGGGTGGCAAATGTGGCCAACCTGGGGAGTCAGGCCGCACAG CCAATGGGCTGCCTGGGCCCCGCAGCCAAGCTCTGTCCAGTTCTTCCGATGAGGGCAGCCCCTACCTTGC TGTAGGAGGGGCACTGGATGCAACCAGGAGCTCTTTGGCTGGCCCAGAACCCTCACTGACCTGGGCAAGG GGGCGGATGGACACACAACCAGACCGTAAACCCTCACGCATACCGACACCTCGGGGACCCCGCCGCCCAT CGGGACCCATAGAACTCGGGGCTTGGCATGCCCAGCATTCGGTCACCCCAAGGACCGAGCCAGATTCTTC GATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209974 representing BC031785 Red=Cloning site Green=Tags(s) MANPVAGIAGSAAKSVRPFRSSEAYVEAMKEDLADWLNALYSLGLPGSGDGFLTGLATGTTLCQHANAVT EAARALAAARPTRGVAFQAHSVAPGSFMARDNVASFIGWCRAELGVPEVLMFETEDLVLRKNEKSVVLCL LEVARRGARLGLLAPRLVQFEQEIERELRATPQVSSVPAAEEDVTEIATVPGVPTRTPRMTPNDLRNLDE LVREILGRCTCPDQFPMIKVSEGKYRVGDSSLLIFVRVLRSHVMVRVGGGWDTLEHYLDKHDPCRCSSST HRLPQQRTGTFSPQRGSPTPSPRPGSPVPGSERRSSRPEVTPISLRGTKEGPETPLRPRDQLPPLPRSRR YSGDSDSSASSAQSGPMGARSDDSATGSRRERPSHRPTSCLPASPRRPTAPRSQSRDRLDRGRPRVAPGG RGAQLSTSSPARRTRSQSREEQAVLMVRRDRDGQHSWVARGRGGGGSGGSGRSTPQTPRALSPAAPRPSR GPSPGPELAATPASIFRTPLQLDPQQEQQLFRRLEEEFLANARALEAAASHTPMGSAPDPPAPDSAYCSS SSSSSSLSVLGGKCGQPGESGRTANGLPGPRSQALSSSSDEGSPYLAVGGALDATRSSLAGPEPSLTWAR GRMDTQPDRKPSRIPTPRGPRRPSGPIELGAWHAQHSVTPRTEPDSSM TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC031785
ORF Size	2034 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC031785, AAH31785
RefSeq Size	2561 bp
RefSeq ORF	2036 bp
Locus ID	78926
Gene Summary	This gene encodes a member of the Gas2 family, actin-associated proteins expressed at high levels in growth-arrested cells. The gene expression is negatively regulated by serum and growth factors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

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