Phactr4 (BC075672) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	3110001B12Rik; AI527228; AW495572; C330013F19Rik; mKIAA4120; N28169
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210087 representing BC075672 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGACCCATCGGAAGAAGCAGAGCAGCCCTCTGGAGACCCAGGCATGGGCATGGACAGTGTGGAAG CAGGGGACACAACACCTCCCACCAAAAGGAAAAGCAAGTTCTCAGCGTTAGGCAAGATCTTCAAGCCGTG GAAATGGAGGAAAAAAAAAAGCAGTGATAAGTTCAAAGAGACATCAGAAGTTTTAGAGCGAAAAATATCT ATGCGAAAACCAAGAGAAGAACTGGTTAAACGAGGGGTTCTGTTGGAGGACCCCGAGCAGGATGGTGAGG ATTCAGGAAAGCTGAGCCATGCTGCATTAAAGAACGGACACACCACCCCCATAGGCAGTGCCAGGTCCTC TAGCCCAGTCCTGGTAGAAGAAGAGCCAGAGAGAAGTCTCAGGAACCTCACTCCAGAAGAGGAGTCAAAG AAGAGACTTGGCTCAACTGGAAGCCAGCCTAACTCTGAAGCCGAGCCTGGTCCTGAGCATGCACCTAAGC AGCCTCTGCTGCCCCCCAAGAGACCCTTGTCCTCTTCTTGTGAAGCAAAGGAGGTTCCGGCTGGCAGTAC AGCAAGGTCCGTCTCCTCCACCTCTGGCTCTACCACCGTGACATCTGCTGCCACCACTGCTGCCACAGAC ATGACAAAGACTGTTAAGTCCTTTGTGGGCCCCACCCCCGCTCCTGCCCCCGCCCCCAGGACTCTGCCTG CTGCTCCTGCCAGTGCTAACACTGCTGCCACCACTACAGCCCCTGCCAAGCAACCCCCCATCCCACCCCC TAAACCAGCTCAGAGAAACAGCAACCCCATCATTGCTGAACTATCCCAAGCAATGAACAGTGGCACAGTG CTGTCGAAACCATCCCCACCCTTGCCACCCAAGAGAGGCATTCCATCAACTTCGATACCCAGCTTGGAGC CTGCTGCTTCGTTCACAACTAAGACAGCAAATGATCAAAGAGAAAAAACTGTCTCTTTGTGTTTGGAACC ACCACTGATAATCCCGCCTTCGTCCCCATCACCCCCACTGCCTACCCACATACCTCCCGAGCCTCCACGG TCCCCTCTAGTGCCTGCTAAGACTTTTCAAATTGTGCCAGAGGTTGAGTTTTCCTCTTCTTCAGATCTAT TCCAGGACATTTCCCAGCAGGAGGATCAGAAAACAGAAGTCCCCAAGAAGATACAGGATCAGAGCTTTGG GGAGTCCCATATACCCTCCAGGCTGCCCCCACTCCCACTGCATATCCGAATTCAGCAGGCCCTTACCAGC CCCCTTCCTGTGACCCCTCCCTTGGAAGGCACTCACAGAGCCCATTCACTGCTTTTTGAGAACAGTGACA GCTTCTCCGAGGACACTGGTACCCTGGGTCGTACCAGGTCACTTCCCATCACGATTGAGATGTTGAAAGT TCCAGATGATGAAGAGGAAGAGCAGACCTGCCCGTTTGTTGAAGACGTGACGTCTACCTCAGCCACTCCT AGCCTACCACTGTGTCTGCGAGAGGAAGAGAAGGAGAGTGACTCAGATTCAGAGGGTCCCATTAAGTACC GAGATGAAGAAGAAGATGATGATGATGATGAAAGCCATCAGAGTGCCCTGGCCAACAGAGTGAAGAGGAA GGACACGCTGGCAATGAAGCTGAGCAGCAGACCCAGTGAACCAGAGACCAACCTGAATTCTTGGCCTCGT AAAAGCAAGGAGGAGTGGAATGAAATACGGCACCAGATCGGGAACACGCTGATCCGGCGGCTGAGTCAGA GACCAACAGCAGAAGAATTGGAGCAGCGGAACATACTACAGCCTAAAAATGAAGCTGATCGGCAAGCAGA GAAACGGGAAATCAAGCGTCGCCTCACTAGAAAGCTCAGTCAAAGGCCAACTGTGGCTGAACTGCTTGCC AGGAAGATTCTGCGGTTTAATGAGTATGTGGAAGTAACTGATGCTCATGACTATGACCGGCGAGCAGACA AACCATGGACCAAACTGACTCCTGCTGACAAGGCTGCCATAAGGAAAGAATTAAATGAATTTAAGAGCTC GGAGATGGAGGTTCATGTAGACAGCAAACACTTTACACGCTACCATCGCCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210087 representing BC075672 Red=Cloning site Green=Tags(s) MEDPSEEAEQPSGDPGMGMDSVEAGDTTPPTKRKSKFSALGKIFKPWKWRKKKSSDKFKETSEVLERKIS MRKPREELVKRGVLLEDPEQDGEDSGKLSHAALKNGHTTPIGSARSSSPVLVEEEPERSLRNLTPEEESK KRLGSTGSQPNSEAEPGPEHAPKQPLLPPKRPLSSSCEAKEVPAGSTARSVSSTSGSTTVTSAATTAATD MTKTVKSFVGPTPAPAPAPRTLPAAPASANTAATTTAPAKQPPIPPPKPAQRNSNPIIAELSQAMNSGTV LSKPSPPLPPKRGIPSTSIPSLEPAASFTTKTANDQREKTVSLCLEPPLIIPPSSPSPPLPTHIPPEPPR SPLVPAKTFQIVPEVEFSSSSDLFQDISQQEDQKTEVPKKIQDQSFGESHIPSRLPPLPLHIRIQQALTS PLPVTPPLEGTHRAHSLLFENSDSFSEDTGTLGRTRSLPITIEMLKVPDDEEEEQTCPFVEDVTSTSATP SLPLCLREEEKESDSDSEGPIKYRDEEEDDDDDESHQSALANRVKRKDTLAMKLSSRPSEPETNLNSWPR KSKEEWNEIRHQIGNTLIRRLSQRPTAEELEQRNILQPKNEADRQAEKREIKRRLTRKLSQRPTVAELLA RKILRFNEYVEVTDAHDYDRRADKPWTKLTPADKAAIRKELNEFKSSEMEVHVDSKHFTRYHRP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC075672
ORF Size	2082 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC075672, AAH75672
RefSeq Size	4549 bp
RefSeq ORF	2084 bp
Locus ID	100169
Gene Summary	Regulator of protein phosphatase 1 (PP1) required for neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. Acts as an activator of PP1 by interacting with PPP1CA and preventing phosphorylation of PPP1CA at 'Thr-320'. During neural tube closure, localizes to the ventral neural tube and activates PP1, leading to down-regulate cell proliferation within cranial neural tissue and the neural retina. Also acts as a regulator of migration of enteric neural crest cells (ENCCs) by activating PP1, leading to dephosphorylation and subsequent activation of cofilin (COF1 or COF2) and repression of the integrin signaling through the RHO/ROCK pathway.[UniProtKB/Swiss-Prot Function]

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