Klhl5 (BC057606) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1300013C10Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210182 representing BC057606 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGTTCTCGCAAAGAGTTTGACGTGAAACAGATTTTGAAAATCAGGTGGAGGTGGTTTGGCCATC AAGCATCGTCTAACTCTTCCGTTGACAGCCAGCAGGGAGAATTTTGGAACCGAGGACAGACCGGAGCAAA CGGTGGGAGAAAGTTTTTAGATCCGTGTAACCTACAATTGCCTTTGGCTTCCATTGGTTACCGAAGGTCC AGCCAACTGGATTTCCAGAATTCACCTTCTTGGCCAATGGCATCCACCTCAGATGTCCCTGCATTTGAGT TTACAGCAGAAGGCTGTGCTGGTGCACACTGGCTGGAGAGACCAGAAGTGGACGATGGCACTAGTGAAGA GGAAAATGAATCTGATTCCAGCTCGAGCAGGACTTCCAATAGTAGTCAGACATTGTCTTCCTGCCAGGCT ATGGAGCCATGCTCATCAGATGAATTCTTTCAAGCCCTTAACCATGCAGAGCAAACATTTAAAAAAATGG AAAACTACTTGAGGCACAAGCAACTGTGTGACGTCATTTTAGTCGCTGGCGACCGCAGGATTCCAGCTCA CAGACTGGTGCTGTCTTCCGTCTCAGATTATTTCGCTGCTATGTTTACTAATGATGTCAGGGAGGCGAGG CAAGAGGAAATCAAAATGGAAGGTGTGGAGCCGAATTCCTTGTGGTCCTTAATTCAGTACGCGTACACAG GCCGACTTGAGCTGAAGGAAGACAACATCGAGTGCTTGCTGTCCACAGCCTGCCTGCTCCAGCTCTCCCA GGTGGTGGAAGCCTGCTGCAAGTTCTTGATGAAGCAGCTCCACCCGTCCAACTGCCTGGGGATTCGGTCC TTCGCGGATGCCCAAGGCTGCACAGATCTGCATAAAGTGGCTCACAATTACACGATGGAACATTTCATGG AAGTAATCAGGAACCAGGAGTTTGTGTTACTCCCAGCCAATGAGATCGCCAAGCTCTTGGCCAGCGATGA CATGAACATCCCGAACGAGGAGACCATACTGAACGCGCTTCTCACCTGGGTCCGGCACGACCTGGAGCAG AGGCGGAAGGATCTCAGTAGACTTTTGGCTTACATTAGGCTACCTCTCCTTGCGCCACAGTTCCTGGCAG ACATGGAAAACAATGCACTTTTCCGGGATGACATTGAATGTCAGAAACTCATCATGGAAGCAATGAAGTA CCATTTATTACCTGAGAGACGACCCATGTTGCAGAGTCCTCGGACAAAGCCTCGCAAGTCAACTGTTGGA ACGTTATTTGCAGTTGGAGGGATGGATTCAACAAAGGGAGCAACAAGCATTGAGAAGTATGACCTCCGTA CAAATATGTGGACTCCTGTGGCAAACATGAATGGGAGGAGGCTCCAATTCGGCGTCGCAGTCTTAGATGA CAAACTGTACGTGGTCGGAGGGCGAGACGGACTGAAGACTCTGAACACCGTAGAGTGCTACAACCCCAAA ACAAAAACCTGGAGTGTGATGCCACCTATGTCCACACACAGGCACGGGCTCGGCGTGGCTGTACTAGAAG GCCCCATGTATGCAGTCGGAGGGCACGACGGCTGGAGCTACCTGAATACTGTGGAGCGATGGGACCCTCA GGCCCGCCAGTGGAATTTTGTTGCCACTATGTCCACCCCCAGGAGCACAGTAGGCGTGGCGGTACTAAGT GGAAAACTCTATGCAGTTGGTGGTCGCGATGGGAGCTCTTGCCTCAAGTCAGTAGAATGCTTTGACCCTC ATACCAATAAATGGACACTGTGTGCCCAGATGTCAAAACGAAGAGGCGGAGTAGGAGTCACAACCTGGAA CGGGCTGCTGTATGCCATTGGGGGACACGACGCTCCCACATCCAATTTGACCTCCAGGCTCTCAGACTGT GTGGAGAGATATGACCCCAAAACAGACGTGTGGACAGCAGTGGCATCCATGAGCGTTAGCAGAGACGCGG TGGGCGTGTGTCTACTTGGTGACAAGCTGTATGCTGTCGGGGGTTATGACGGACAGACCTACCTGAACAT AGTGGAGGCTTACGACCCCCAGACCAACGAATGGACCCAGGTTGCTCCGCTGTGCCTAGGAAGAGCTGGA GCCTGTGTTGTGACTGTGAAGATA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210182 representing BC057606 Red=Cloning site Green=Tags(s) MSGSRKEFDVKQILKIRWRWFGHQASSNSSVDSQQGEFWNRGQTGANGGRKFLDPCNLQLPLASIGYRRS SQLDFQNSPSWPMASTSDVPAFEFTAEGCAGAHWLERPEVDDGTSEEENESDSSSSRTSNSSQTLSSCQA MEPCSSDEFFQALNHAEQTFKKMENYLRHKQLCDVILVAGDRRIPAHRLVLSSVSDYFAAMFTNDVREAR QEEIKMEGVEPNSLWSLIQYAYTGRLELKEDNIECLLSTACLLQLSQVVEACCKFLMKQLHPSNCLGIRS FADAQGCTDLHKVAHNYTMEHFMEVIRNQEFVLLPANEIAKLLASDDMNIPNEETILNALLTWVRHDLEQ RRKDLSRLLAYIRLPLLAPQFLADMENNALFRDDIECQKLIMEAMKYHLLPERRPMLQSPRTKPRKSTVG TLFAVGGMDSTKGATSIEKYDLRTNMWTPVANMNGRRLQFGVAVLDDKLYVVGGRDGLKTLNTVECYNPK TKTWSVMPPMSTHRHGLGVAVLEGPMYAVGGHDGWSYLNTVERWDPQARQWNFVATMSTPRSTVGVAVLS GKLYAVGGRDGSSCLKSVECFDPHTNKWTLCAQMSKRRGGVGVTTWNGLLYAIGGHDAPTSNLTSRLSDC VERYDPKTDVWTAVASMSVSRDAVGVCLLGDKLYAVGGYDGQTYLNIVEAYDPQTNEWTQVAPLCLGRAG ACVVTVKI SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene
ACCN	BC057606
ORF Size	2124 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC057606, AAH57606
RefSeq Size	3265 bp
RefSeq ORF	2126 bp
Locus ID	71778

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