Phtf1 (BC050825) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Phtf, Phft
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210245 representing BC050825 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCAAATGAGAGAGATGCTATATCATGGTATCAGAAGAAGATTGGAGCATATGATCAGCAGATAT GGGAAAAGTCAATCGAACAGACTCAGATTAAGGGTTTCAAAAACAAACCAAAAAAAATGGGCCATATAAA ACCAGACTTGATTGATGTTGACTTAATCAGAGGCTCAACATTTGCCAAAGCCAAGCCTGAGATTCCATGG ACCTCGCTCACTCGGAAGGGGCTTGTTCGAGTGGTGTTTTTCCCGTTGTTCAGCAGCTGGTGGATTCAGG TTACCTCTCTCAGAATCTTTGTTTGGTTGCTGCTACTTTACCTCATGCAAGTCACAGCGGTTGTGTTGTA TCTCCTGATGCCTATTGTGAGCGCCAGTGAAGTGCTTGGACCCTTGTGCCTTATGCTACTCATGGGAACT GTCCACTGTCAGATTGTGTCGACTCAGATAACAAGGCCGTCAGGAAACAATGGAAATCGGAGAAGAAGGA TTAAAAGGGTGAAATTAGTATCTAACAAAGGTACTGAAACTGACAATGACTCAGGCTGTTTCCATCCTAT CCTTAAGAAGAGACAGGGTCGACCCGAGATCAGAATGTGGCAAGCAAGAGAGAAAGCAAAGGTTTCGGAT GGAGAAAAGTGCCGTAGGGAGGCTTATAGGCGTTTGGGTAATGGAATTTCAGACGATCTGTCAAGTGAGG AGGATGGTGAAGCACGGACACAGATGATATTATTGCGTAGGAGTGTGGAAGGGGCGTCAAGTGACAATGG TTATGAAGTTAAGAATAGAAGGTCAATACTTTCAAGGCACCTAAATTCTCAGGTAAAGAAAACCACAAGG TGGTGTCATATTGTTCGGGATTCAGATAGTCTGGCTGAATCAGAGTTTGAATCAGCAGTCTTCAGCCAGG GTTCTAGATCTGGTATGAGTGGTGGCTCTCGGAGCCTTAACTTGTCAAGAAGAGACTCAGAAAGCACCCG ACATGACTCCGAGACTGAGGATATGTTATGGGATGACCTTCTCCATGGTCCAGAGTGCCGGTCATCTGTC ACCAGTGACAGTGAGGGGGCTCATGTAAATACCATTCATTCAGGGACCAAACGTGACCCCAAAGAAGATG TTTTTCAGCAGAACCATTTATTCTGGCTTCAGAATTCAAGTCCTGCCTCTGAGCGAGTTAGTGCGATAAT CTGGGAAGGAAACGAGTGCAAAAAGATGGACATGTCTGTGTTGGAGATAAGCGGCATCATCATGAGCAGG GTCAATGCATACGAGCAAGGTGTTGGCTATCAGATGCTGGGGAATGCAGTCACCGTTGGCTTAGCACTTT TCCCATTTTTATATCGACTTTTCCGAGAGAAGAGCTTTGACCAACTAAAGTCCATCTCAGCAGAGGAGGT TCTGACCCTCTTCTGTGGTGCGCCACCCGTCACACCTGTTGTTATTTTGTCTATAATTAATTTTATTGAA AGATTGTGTCTTACGTGGATGTTTTTTTTCATGATGTGTGTGGCAGAGAGAACATATAAGCAGAGATTTT TATTTGCAAAACTCTTCAGCCATATTACTTCTGCCAGGAAGGCCAGGAAATATGAAATACCTCATTTCAG ACTTAAAAAGGTGGAGAACATTAAAATATGGTTATCCCTGCGCTCCTATCTAAAGAGGCGGGGTCCACAG CGCTCAGTGGACGTGGTTGTGTCCTCCGTCTTCTTACTGACACTTTCAATTGCTTTCATTTGTTGTGCAC AGGTTCTTCAAGGTCACAAGACATTCCTGAATGATGCTTACAACTGGGAGTTCTTGATCTGGGAAACAGC ATTGCTGCTGTTTTTGCTTCGCCTGGCCTCACTGGGGTCAGAAACCAACAAGAAGTACAGCAATGTTTCC ATCCTGCTCACTGAACAGATTAATTTATATCTTAAAATGGAAAAAAAGCCAAATAAGAAAGAACAGCTCA CGCTAGTGAATAATGTATTGAAGCTGTCCACAAAGCTGTTAAAGGAGCTGGACACACCATTTAGACTCTA TGGACTGACCATGAACCCCTTAATCTACAACATCACAAGAGTAGTCATCCTTTCTGCTGTCTCTGGAGTT ATAAGCGACCTTCTAGGATTCAACATAAGACTGTGGAAAATTAAGTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210245 representing BC050825 Red=Cloning site Green=Tags(s) MASNERDAISWYQKKIGAYDQQIWEKSIEQTQIKGFKNKPKKMGHIKPDLIDVDLIRGSTFAKAKPEIPW TSLTRKGLVRVVFFPLFSSWWIQVTSLRIFVWLLLLYLMQVTAVVLYLLMPIVSASEVLGPLCLMLLMGT VHCQIVSTQITRPSGNNGNRRRRIKRVKLVSNKGTETDNDSGCFHPILKKRQGRPEIRMWQAREKAKVSD GEKCRREAYRRLGNGISDDLSSEEDGEARTQMILLRRSVEGASSDNGYEVKNRRSILSRHLNSQVKKTTR WCHIVRDSDSLAESEFESAVFSQGSRSGMSGGSRSLNLSRRDSESTRHDSETEDMLWDDLLHGPECRSSV TSDSEGAHVNTIHSGTKRDPKEDVFQQNHLFWLQNSSPASERVSAIIWEGNECKKMDMSVLEISGIIMSR VNAYEQGVGYQMLGNAVTVGLALFPFLYRLFREKSFDQLKSISAEEVLTLFCGAPPVTPVVILSIINFIE RLCLTWMFFFMMCVAERTYKQRFLFAKLFSHITSARKARKYEIPHFRLKKVENIKIWLSLRSYLKRRGPQ RSVDVVVSSVFLLTLSIAFICCAQVLQGHKTFLNDAYNWEFLIWETALLLFLLRLASLGSETNKKYSNVS ILLTEQINLYLKMEKKPNKKEQLTLVNNVLKLSTKLLKELDTPFRLYGLTMNPLIYNITRVVILSAVSGV ISDLLGFNIRLWKIKS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC050825
ORF Size	2148 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC050825, AAH50825
RefSeq Size	3025 bp
RefSeq ORF	2150 bp
Locus ID	18685
Gene Summary	May play a role in transcription regulation.[UniProtKB/Swiss-Prot Function]

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