Rgl1 (BC017678) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Rgl, mKIAA0959
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210552 representing BC017678 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATTACTTTGGCAAGCTAAAATGAGCTCGATTCAGGACTGGGGTGAAGAGGTAGAGGAAGGAGCTG TTTACCATGTCACCCTCAAAAGAGTCCAGATTCAACAGGCGGCCAATAAAGGAGCGAGATGGCTAGGGGT TGAAGGGGACCAGCTGCCTCCAGGACACACAGTCAGTCAGTACGAGACCTGCAAGATCAGGACCATCAAA GCTGGTACGCTGGAGAAGCTTGTGGAGAACCTGCTGACGGCTTTTGGGGACAATGACTTTACCTACATCA GCATCTTTTTGTCGACATACAGAGGCTTTGCCTCGACTAAGGAAGTGCTGGAGCTGCTGCTGGACAGGTA TGGAAACCTGACAGGCCCAAACTGTGAAGACGATGGAAGCCAAAGTTCACCCGAGTCCAAGGCCGTGATC CGGAATGCCATTGCTTCCATCCTGAGGGCCTGGCTTGACCAGTGTGCGGAAGACTTCCGGGAGCCCCCTC ACTTCCCTTGCCTTCAGAAGCTGCTGGAGTACCTCAAACAGATGATGCCTGGCTCTGACCCAGAGAGGAG AGCACAGAACCTTCTTGAACAGTTTCAAAAGCAGGACGTGGATTCCGACAATGGACTTCTCAACACCAGC TCCTTCAGCCTGGAAGAGGAAGAGGAACTGGAGAGCGGAGGGTCAGCAGAATTCACGAACTTCTCAGAAG ATCTCGTGGCAGAACAGCTGACCTACATGGACGCACAACTATTCAAGAAGGTAGTGCCTCACCATTGCCT GGGCTGTATTTGGTCTCAGCGGGATAAAAAGGAAAACAAGCATTTGGCTCCTACGATCCGTGCCACCATC TCTCAGTTTAATACGCTCACCAAGTGTGTTGTCAGCACCGTCCTGGGGAGCAAGGAACTCAAAACTCAGC AGCGAGCCAGAGTCATCGAGAAGTGGATCAACATTGCTCACGAATGTAGAATCCTGAAGAATTTTTCCTC CTTGAGGGCCATCGTTTCCGCACTGCAGTCTAATTCCATCTATCGGTTGAAAAAGGCTTGGGCTGCTGTC CCGAAGGACAGAATGCTGATGTTTGAAGAACTTTCAGATATCTTCTCTGATCACAATAACCATCTAACCA GTCGGGAGCTACTAATGAAGGAAGGAACTTCAAAATTTGCAAACCTGGACAGCAGCGTGAAAGAAAACCA GAAGCGGACCCAGAGGCGCCTGCAACTGCAGAAGGATATGGGTGTGATGCAGGGTACCGTGCCTTACCTG GGCACCTTCCTGACTGACCTGACCATGCTGGACACTGCCCTGCAGGACTACATTGAGGGTGGACTGATCA ACTTCGAGAAAAGAAGAAGGGAATTTGAAGTCATTGCCCAGATAAAGCTCCTACAGTCTGCTTGCAACAG CTACTGCATGGGCCCAGACCAGAAGTTTATCCAGTGGTTCCAGAGGCAGCAGCTTCTATCAGAGGAGGAA AGCTACGCCCTCTCGTGTGAGATTGAAGCCGCTGCCGACGCCAACACCACTTCCCCTAAGCCTCGGAAAA GCATGGTGAAGAGGCTGAGCCTGCTATTTCTGGGGTCTGACATCATCCCCGGGAGCACTCCCACCAAAGA GCAGCCCAAGTCCGCAGCCAGTGGGAGCTCTGGGGAGAGTATGGACTCAGTCAGTGTGTCGTCCTGTGAA TCAAACCACTCCGAGGCTGAGGAGGGCCCCGTCACACCCATGGACACACCAGATGAGCCCCAAAAGAAGC TCTCTGAATCCTCCTCTTCCTGTTCCTCCATCCATTCCATGGACACGAATTCCTCAGGGATGTCGTCCCT AATCAACCCCCTGTCCTCCCCTCCAACGTGCAACAACAATCCTAAAATCCACAAGCGCTCCGTCTCCGTG ACATCCATTACCTCCACAGTACTGCCTCCTGTTTACAATCAGCAGAACGAAGACACCTGCATCATCCGCA TCAGTGTAGAAGACAACAATGGCAACATGTACAAGAGCATCATGCTGACAAGCCAGGATAAGACCCCCGC TGTGATCCAGAGAGCGATGTCGAAGCACAACCTGGAGTCGGACCCCGCCGAGGAGTATGAGCTGGTGCAG GTCATCTCGGAGGACAAAGAACTAGTGATCCCGGACTCTGCAAACGTCTTTTACGCCATGAATAGCCAAG TGAACTTTGATTTCATTTTACGCAAAAAGAACTCGGTGGAGGAGCAGGTGAAGTTGCGCAGTCGGACCAG CCTGACTTTGCCCAGGACAGCTAAGCGGGGCTGCTGGAGTAACAGGCACAGCAAGATCACCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210552 representing BC017678 Red=Cloning site Green=Tags(s) MKLLWQAKMSSIQDWGEEVEEGAVYHVTLKRVQIQQAANKGARWLGVEGDQLPPGHTVSQYETCKIRTIK AGTLEKLVENLLTAFGDNDFTYISIFLSTYRGFASTKEVLELLLDRYGNLTGPNCEDDGSQSSPESKAVI RNAIASILRAWLDQCAEDFREPPHFPCLQKLLEYLKQMMPGSDPERRAQNLLEQFQKQDVDSDNGLLNTS SFSLEEEEELESGGSAEFTNFSEDLVAEQLTYMDAQLFKKVVPHHCLGCIWSQRDKKENKHLAPTIRATI SQFNTLTKCVVSTVLGSKELKTQQRARVIEKWINIAHECRILKNFSSLRAIVSALQSNSIYRLKKAWAAV PKDRMLMFEELSDIFSDHNNHLTSRELLMKEGTSKFANLDSSVKENQKRTQRRLQLQKDMGVMQGTVPYL GTFLTDLTMLDTALQDYIEGGLINFEKRRREFEVIAQIKLLQSACNSYCMGPDQKFIQWFQRQQLLSEEE SYALSCEIEAAADANTTSPKPRKSMVKRLSLLFLGSDIIPGSTPTKEQPKSAASGSSGESMDSVSVSSCE SNHSEAEEGPVTPMDTPDEPQKKLSESSSSCSSIHSMDTNSSGMSSLINPLSSPPTCNNNPKIHKRSVSV TSITSTVLPPVYNQQNEDTCIIRISVEDNNGNMYKSIMLTSQDKTPAVIQRAMSKHNLESDPAEEYELVQ VISEDKELVIPDSANVFYAMNSQVNFDFILRKKNSVEEQVKLRSRTSLTLPRTAKRGCWSNRHSKITL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC017678
ORF Size	2304 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC017678, AAH17678
RefSeq Size	3908 bp
RefSeq ORF	2306 bp
Locus ID	19731
Gene Summary	This gene encodes a member of the Ras-like (Ral) -selective guanine nucleotide exchange factor (RalGEF) family of small GTPase activators which function both as downstream effectors of activated Ras GTPase and as regulators of certain Ral GTPases in the RalGEF - Ral GTPase signaling pathway. The encoded protein, like other RalGEFs, has an N-terminal ras exchanger motif domain, a catalytic CDC25 homology domain, and a C-terminal ras binding domain that stimulates guanine nucleotide exchange when bound to a Ral GTPase. RalGEF family members bridge the Ras and Ral signaling pathways and are thought to play a role in oncogenic transformation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.