Brsk1 (BC086636) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	SADB, SAD-B
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210598 representing BC086636 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCCGGGTCCAAGGAAGGTGGCGGGGGCTCCCCCGCCTACCACCTCCCACACCCACACCCACACC CACCCCAGCACGCCCAATATGTGGGCCCCTATCGCCTGGAGAAGACGCTGGGCAAAGGACAGACAGGTCT AGTTAAACTTGGAGTCCACTGCATCACGGGTCAGAAGGTCGCTGTCAAGATCGTGAACAGGGAGAAGCTG TCGGAATCGGTGCTGATGAAGGTGGAGAGGGAAATCGCCATCCTGAAGCTCATTGAACACCCGCACGTGC TCAAGCTCCACGACGTCTACGAGAACAAGAAATATTTATACTTGGTTCTTGAGCACGTTTCTGGTGGTGA GCTGTTCGACTACCTGGTAAAAAAAGGGAGACTGACACCCAAGGAGGCCCGGAAGTTCTTCCGCCAGATC GTGTCAGCACTGGACTTCTGCCATAGCTACTCCATCTGTCACAGAGACTTGAAGCCAGAGAACCTGCTGT TGGATGAGAAAAACAACATCCGCATCGCAGACTTTGGTATGGCGTCCCTGCAAGTGGGGGACAGCCTCCT GGAGACCAGCTGCGGGTCCCCCCATTACGCATGTCCAGAGGTGATCAAGGGGGAAAAGTATGATGGCCGC CGGGCAGACATGTGGAGCTGTGGAGTCATCCTATTTGCCCTGCTTGTGGGGGCACTGCCCTTCGATGACG ACAACCTGCGCCAGCTACTGGAGAAGGTGAAACGTGGGGTCTTCCACATGCCTCACTTCATCCCTCCAGA CTGCCAGAGCCTCCTGAGAGGGATGATTGAAGTGGAGCCCGAGAAAAGGCTCAGTCTGGAGCAAATTCAG AAACATCCTTGGTATCTGGGCGGGAAACACGAACCAGACCCTTGCCTGGAGCCAGCCCCAGGCCGCAGAG TAGCCATGCGTAGCCTGCCTTCCAATGGCGAGCTGGACCCTGACGTTCTGGAAAGCATGGCGTCTCTGGG CTGCTTCAGAGACCGCGAGCGGCTGCACAGAGAACTGCGAAGCGAGGAGGAAAACCAAGAAAAGATGATC TATTATTTGCTTTTGGATCGGAAAGAGCGGTATCCTAGCTGTGAAGACCAGGACCTGCCTCCTCGGAATG ATGTTGACCCACCTCGTAAGCGTGTGGATTCCCCCATGCTGAGCAGACACGGGAAACGGAGGCCAGAGCG GAAGTCCATGGAAGTCCTCAGTATCACAGATGCTGGGAGTGGTGGCTCCCCAGTGCCTACCAGACGGGCC TTGGAGATGGCCCAGCACAGTCAGAGATCCCGCAGCGTCAGTGGAGCCTCCACTGGTCTGTCCTCCAGCC CTCTGAGCAGCCCAAGGAGTCCGGTCTTTTCCTTCTCACCGGAGCCAGGCGCTGGAGATGAGGCTAGAGG CGGAGGCTCCCCAACTTCCAAAACACAGACGCTGCCTTCTCGGGGCCCCAGAGGTGGGGGCGCTGGAGAG CAGCCACCACCCCCCAGTGCCCGTTCCACGCCCTTGCCTGGACCACCAGGCTCCCCGCGCTCCTCCGGCG GAACCCCCTTGCACTCACCTCTGCACACGCCTCGAGCCAGCCCTACGGGGACCCCAGGAACGACGCCACC TCCCAGCCCAGGCGGTGGCGTCGGGGGAGCCGCCTGGAGAAGTCGGCTCAACTCCATCCGCAACAGCTTC CTGGGCTCCCCTCGCTTCCACCGGCGCAAGATGCAGGTCCCTACCGCCGAGGAGATGTCCAGTTTGACAC CAGAATCCTCTCCTGAGCTGGCAAAACGCTCCTGGTTCGGGAATTTCATCTCCTTGGACAAAGAAGAACA AATATTCCTCGTGCTAAAGGACAAACCTCTCAGCAGCATCAAAGCGGACATTGTCCATGCCTTTCTGTCG ATCCCCAGCCTGAGTCACAGTGTACTGTCACAGACCAGCTTCAGGGCCGAATACAAGGCCAGCGGCGGCC CCTCCGTCTTCCAGAAGCCTGTCCGCTTTCAGGTGGACATCAGCTCCTCTGAGGGTCCAGAACCCTCACC CCGAAGAGATGGCAGCAGTGGAGGTGGCATCTACTCAGTCACCTTTACTCTCATCTCCGGTCCCAGCCGT CGGTTCAAACGTGTGGTAGAGACCATCCAGGCACAGTTGCTGAGTACTCACGACCAGCCCTCCGTGCAGG CCTTGGCAGATGAGAAGAACGGAGCCCAGACCCGGCCTGCTGGGACCCCACCCCGAAGCCTGCAGCCCCC ACCAGGCCGCTCTGACCCTGATCTGAGTAGCTCTCCCCGCCGAGGACCCCCTAAGGACAAGAAGCTCCTG GCCACCAATGGAACCCCTCTACCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210598 representing BC086636 Red=Cloning site Green=Tags(s) MSSGSKEGGGGSPAYHLPHPHPHPPQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAVKIVNREKL SESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQI VSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGR RADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQ KHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMI YYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGSGGSPVPTRRA LEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGE QPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSF LGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLS IPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSSGGGIYSVTFTLISGPSR RFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGTPPRSLQPPPGRSDPDLSSSPRRGPPKDKKLL ATNGTPLP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC086636
ORF Size	2334 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC086636, AAH86636
RefSeq Size	3013 bp
RefSeq ORF	2336 bp
Locus ID	381979
Gene Summary	Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-504' and 'Ser-554'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.[UniProtKB/Swiss-Prot Function]

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