Rnf10 (BC010342) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AA675014; mKIAA0262; RIE2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210752 representing BC010342 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCAGAGCTCCCCCAGCGCCGCCGCCACCGCCTCCGACATGGACAAGAACAGCGGCTCCAACAGCT CCTCCGCCTCCTCGGGCAGCAGCAAAGGGCAACAGCCGCCCCGCTCCGCCTCGGCGGGGCCGGCCGGCGA GTCTAAACCCAAGAGTGATGGAAAGAACTCAAATGGATCCAAGCGTTACAATCGCAAGCGTGAACCCTCC TACCCCAAAAATGAAAATTTTAGCAACCAGTCCCGTCGCTCCAATTCACAGAAAAGCAAAACTTTTAACA AGATGCCTCCTCAAAGGGGCGGTGGCAGCAGCAAACCCTTTAGCTCTTCTTCTAATGGTGGAAGACGAGA TGAGGTAGCAGAGGCTCAGCGGGCAGAGTTTAGCCCTGCCCAGTTCTCTGGCCCTAAGAAGATCAACCTG AACCACTTGTTAAATTTCACCTTTGAACCACGTGGCCAGGCAGGTCACTTTGAAGGCAGTGGGCATGGCG GCTGGGGGAAGAGAAACAAGTGGGGACACAAGCCTTTCAACAAGGAGCTCTTTCTACAGGCCAACTGCCA ATTTGTGGTGTCTGAAGACCAAGACTACGCAGCTCACTTTGCTGACCCTGACACCTTAGTCAACTGGGAC TTTGTGGAACAAGTGCGCATCTGTAGCCATGAAGTGCCATCTTGCCCAATATGCCTCTATCCACCTACTG CGGCCAAGATAACGCGCTGTGGACACATTTTCTGCTGGGCATGCATCCTGCACTATCTTTCACTCAGTGA GAAAACCTGGAGCAAATGTCCCATCTGCTACAGCTCTGTGCATAAGAAGGATCTCAAGAGTGTTGTTGCC ACAGAGTCTCGACAGTATGCTGTGGGGGACACCATTACCATGCAGCTGATGAAGAGAGAGAAGGGAGTGT TGGTGGCCCTGCCCAAATCCAAGTGGGTGAATGTAGACCATCCTATTAACCTAGGAGATGAGCAGCTCAG CCAGTATTCCAAGCTGCTGCTGGCCTCGAAGGAGCAGGTGCTGCACCGGGTAGTTCTGGAGGAGAAAGGA GCGCTGGAGCAGCAGCTGGCAGAGGAGAAGCACACTCCTGAGTCCTGTTTCATTGAGGCTGCTATCCAGG AGGTCAAGATTCGGGAAGAAGCTCTGTCAGGAGTGGCTGGAGGCGGAGGGGAGGTCACTGGTGTTGTGGC TGCTCTGGAACACCTGGTGCTGATGGCTCCCCTGGCCACGGAGTCTGCTTTCCAACCCAGGAAGGGTGTG CTGGAGTATCTGTCTGCGTTTGATGATGAAGCTGCACAAGTTTGTTCTCTGGACCCCCCTGGTCCTCTTG CCCTCCCTTTGGTAGAAGAGGAGGAAGCAGTGTCTGAGCCAGAAGCCTGTGAGGACGCAGAGGTAGCCGA TGACAGTCTGGGAGAGGGGACCGTTGGTCCTGAGATGAGCCAGGAGGAGCCCATCACTAAGCCAGGCTTC ACCCAGCTCAGCAGCTCTCCGTGTTACTACTTCTACCAGGCGGAAGATGGACAGCACATGTTCCTGCACC CTGTCAACGTGCGCTGCCTTGTGCGGGAGTACGGCAGCCTGGAGCAGAGCCCAGAGAAGATCTCAGCAAC CGTGGTAGAGATTGCTGGGTATTCCATGTCTGAGGATGTTCGGCAGCGTCATAGGTATCTCTCTCACTTG CCACTCACCTGTGAGTTCAGCATCTGTGAACTGGCTCTGCAGCCTCCTGTGGTCTCTAAGGAAACCCTAG AGATGTTCTCAGATGACATTGAGAAAAGGAAGCGTCAGCGCCAAAAGAAGGCTCGCGAGGAGCGTCGCCG AGAGCGCAGAATTGAGATGGAGGAGAACAAGAGACAGGGCAGGTATCCCGAGGTCCACATTCCCCTCGAG AACCTACAGCAGTTTCCGGCCTTCAATTCCTATACCTGTCCCTCTGATTCTGCTTTGGGTCCCACCAGCA CTGAGGGCCATGGGGCCCTATCCCTGTCTCCTCTGAGCAGAAGTCCAGGCTCCCATGCAGACTTTCTGCT GACCCCTCTGTCACCCACTGCCAGTCAGGGCAGTCCTTCCTTCTGCGTTGGGAGTCTGGAAGACGACTCT CCCTTCCTTTCCTTTGCCCAGATGCTGAGGGTTGGCAAAGCAAAAGCAGACGGGTGGCCCAAAACTGCTC CAAAGAAAGATGACAATAGCTTGGTTCCTCCCGCCCCTGTGGACAGTGATGGGGAGAGTGACAATTCTGA CCGTGTTCCTGTGCCCAGTTTCCAAAATTCCTTCAGCCAAGCTATCGAAGCAGCCTTCATGAAATTGGAC ACACCAGCTACTTCAGACCCCCTCTCTGAAGACAGAGGAGGGAAGAAAAGGAAGAGACAGAAGCAGAAGC TGCTGTTCAGCACCTCGGTCGTCCACACCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210752 representing BC010342 Red=Cloning site Green=Tags(s) MPQSSPSAAATASDMDKNSGSNSSSASSGSSKGQQPPRSASAGPAGESKPKSDGKNSNGSKRYNRKREPS YPKNENFSNQSRRSNSQKSKTFNKMPPQRGGGSSKPFSSSSNGGRRDEVAEAQRAEFSPAQFSGPKKINL NHLLNFTFEPRGQAGHFEGSGHGGWGKRNKWGHKPFNKELFLQANCQFVVSEDQDYAAHFADPDTLVNWD FVEQVRICSHEVPSCPICLYPPTAAKITRCGHIFCWACILHYLSLSEKTWSKCPICYSSVHKKDLKSVVA TESRQYAVGDTITMQLMKREKGVLVALPKSKWVNVDHPINLGDEQLSQYSKLLLASKEQVLHRVVLEEKG ALEQQLAEEKHTPESCFIEAAIQEVKIREEALSGVAGGGGEVTGVVAALEHLVLMAPLATESAFQPRKGV LEYLSAFDDEAAQVCSLDPPGPLALPLVEEEEAVSEPEACEDAEVADDSLGEGTVGPEMSQEEPITKPGF TQLSSSPCYYFYQAEDGQHMFLHPVNVRCLVREYGSLEQSPEKISATVVEIAGYSMSEDVRQRHRYLSHL PLTCEFSICELALQPPVVSKETLEMFSDDIEKRKRQRQKKAREERRRERRIEMEENKRQGRYPEVHIPLE NLQQFPAFNSYTCPSDSALGPTSTEGHGALSLSPLSRSPGSHADFLLTPLSPTASQGSPSFCVGSLEDDS PFLSFAQMLRVGKAKADGWPKTAPKKDDNSLVPPAPVDSDGESDNSDRVPVPSFQNSFSQAIEAAFMKLD TPATSDPLSEDRGGKKRKRQKQKLLFSTSVVHTK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC010342
ORF Size	2412 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC010342, AAH10342
RefSeq Size	2840 bp
RefSeq ORF	2414 bp
Locus ID	50849
Gene Summary	The protein encoding this gene is a member of the really interesting new gene finger protein family. Members of this family contain protein motifs similar to zinc finger domains and are involved in many processes that include transcriptional regulation, DNA repair and signal transduction. Expression of this gene is upregulated during neuronal differentiation of cultured cells, and inhibition of its expression impairs differentiation and cell cycle exit, providing evidence for a function in neuronal differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

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