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Mcm6 (NM_008567) Mouse Tagged ORF Clone
CAT＃: MG210820
- TrueORF®
Mcm6 (tGFP-tagged) - Mouse minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae) (Mcm6)
ORF Plasmid: DDK
"NM_008567" in other vectors (4)
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CNY 7,980.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ASP-l1; D1Wsu22e; Mcmd6 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210820 representing NM_008567
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCTCGCAGCGGCCGCGGAGCCCGGCGCCGGAAGCCAGCACCCGGAGGTGCGCGACGAGGTGGCCG AGAAATGCCAGAAGCTGTTCCTAGACTTCCTGGAAGAGTTCCAGGGTAGTGATGGAGAAATTAAATACTT GCAGTTTGCAGAGGAGCTAATTCGTCCTGAGAGAAACACGCTGGTTGTGAGTTTTGCAGACCTGGAACAA TTTAACCAACAACTTTCTACCACCATTCAGGAAGAGTTCTATAGAGTCTACCCTTACCTGTGTCGAGCCT TGAAGACCTTTGTCAAAGACCGAAAGGAGATCCCTTTTGCTAAGGATTTTTATGTTGCATTCCAAGACCT ACCTACTAGACACAAGATTCGTGAGCTCACTTCATCCAGGATTGGGCTGCTCACCCGAATCAGTGGCCAG GTGGTGCGCACTCACCCAGTTCACCCTGAGCTTGTGAGTGGAACTTTCCTGTGCCTTGACTGTCAAACAG TGATTAAGGACGTAGAGCAGCAGTTCAAATACACACAGCCGAATATCTGCCGAAATCCAGTGTGTGCCAA CAGGAAGAGGTTCCTTCTAGACACTAATAAATCAAGATTTGTTGATTTTCAAAAGGTTCGCATTCAAGAG ACTCAAGCGGAACTTCCCCGGGGAAGTATTCCCCGGAGTTTAGAGGTTATCCTGAGAGCTGAAGCTGTGG AGTCAGCCCAAGCTGGTGACAGATGTGATTTCACAGGGGCACTGATTGTTGTGCCTGATGTCTCCAAGCT TAGCACACCAGGAGCACGTGCAGAGACTAACTCCCGAGTCAGTGGAGCTGATGGGTATGAGACAGAAGGC ATTCGAGGGCTCCGGGCCCTTGGTGTCAGAGATCTGTCATACAGGCTGGTCTTCCTTGCCTGCCATGTTG CACCAACCAACCCAAGGTTTGGAGGGAAAGAACTCAGAGATGAGGAACAGACCGCTGAGAGCATTAAGAA CCAAATGACGGTGAAGGAATGGGAAAAGGTGTTTGAAATGAGTCAGGACAAAAACCTGTACCACAATCTC TGCACGAGCCTCTTCCCTACTATACATGGCAATGATGAAGTAAAACGCGGTGTCTTACTGATGTTGTTCG GTGGTGTTCCTAAGACAACAGGGGAAGGAACCTCTCTTCGTGGGGATATAAATGTTTGCATTGTTGGTGA TCCAAGTACAGCTAAGAGCCAATTTCTCAAGCACGTGGACGAGTTCAGTCCCAGAGCTGTCTATACCAGT GGCAAAGCCTCCAGCGCTGCCGGCTTAACAGCAGCTGTGGTGAGAGATGAAGAATCTCACGAATTTGTCA TTGAGGCTGGAGCGTTGATGCTGGCTGATAATGGTGTCTGTTGTATTGATGAATTTGATAAGATGGACAT GCGGGACCAAGTTGCTATTCACGAAGCTATGGAGCAGCAGACCATCTCTATCACGAAAGCAGGAGTGAAG GCAACTCTGAATGCCAGGACATCCATTTTAGCTGCAGCAAACCCTGTCAGCGGACACTATGACAGATCAA AATCTTTGAAACAGAACATCAATCTGTCAGCGCCCATCATGTCCCGCTTTGATCTCTTCTTCATTCTGGT GGATGAATGCAATGAGGTGACAGATTATGCTATTGCCAGGCGCATAGTAGACTTGCATTCAAGAATTGAA GAATCGATCGATCGAGTTTATTCCCTTGATGATATAAGAAGGTACCTTCTCTTTGCCCGACAGTTTAAAC CCAAGATTTCCAAAGAGTCAGAAGACTTCATTGTGGAGCAGTACAAACGTCTCCGCCAGAGGGATGGCTC TGGGGTCACCAAGTCCTCGTGGAGAATCACCGTGCGACAGCTTGAGAGCATGATCCGACTCTCAGAATCA ATGGCCCGCATGCACTGCTGTGACGAGGTTCAGCCTAAACATGTGAAGGAAGCTTTTCGATTACTGAATA AATCAATCATCCGTGTAGAGACGCCTGATGTCAATCTAGATCAAGAGGAAGAGATCCAGATGGAGACGGA TGAGGGACAAGGAGGCGTCAATGGTCATGCCGACAGCCCTGCCCCTGTGAATAGGTTCAACGGCTCCAGT GAAGATGCCAGCCAGGAGACTGTTTCCAAGCCCTCCCTGAGGCTGGGCTTTGCTGAATACTGCCGAATCT CGAACCTCATCGTGCTCCACCTCAGGAAAATGGAAGAAGAGGAGGACGAGTCGGCACTAAAGAGAAGCGA GCTTGTCAACTGGTACCTGAAGGAAATCGAATCAGAGATAGACTCTGAAGAAGAGCTCATAAATAAAAAG ACGATCATTGAGAAGGTTGTTCATCGTCTCACACACTACGATCACGTTCTGATTGAGCTCACCCAGGCTG GGCTGAAAGGCTCCAGTGAAGGAAGCGAGAGCTATGAGGAAGACCCCTACCTGGTAGTCAACCCTAACTA CTTGCTTGAAGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210820 representing NM_008567
Red=Cloning site Green=Tags(s) MDLAAAAEPGAGSQHPEVRDEVAEKCQKLFLDFLEEFQGSDGEIKYLQFAEELIRPERNTLVVSFADLEQ FNQQLSTTIQEEFYRVYPYLCRALKTFVKDRKEIPFAKDFYVAFQDLPTRHKIRELTSSRIGLLTRISGQ VVRTHPVHPELVSGTFLCLDCQTVIKDVEQQFKYTQPNICRNPVCANRKRFLLDTNKSRFVDFQKVRIQE TQAELPRGSIPRSLEVILRAEAVESAQAGDRCDFTGALIVVPDVSKLSTPGARAETNSRVSGADGYETEG IRGLRALGVRDLSYRLVFLACHVAPTNPRFGGKELRDEEQTAESIKNQMTVKEWEKVFEMSQDKNLYHNL CTSLFPTIHGNDEVKRGVLLMLFGGVPKTTGEGTSLRGDINVCIVGDPSTAKSQFLKHVDEFSPRAVYTS GKASSAAGLTAAVVRDEESHEFVIEAGALMLADNGVCCIDEFDKMDMRDQVAIHEAMEQQTISITKAGVK ATLNARTSILAAANPVSGHYDRSKSLKQNINLSAPIMSRFDLFFILVDECNEVTDYAIARRIVDLHSRIE ESIDRVYSLDDIRRYLLFARQFKPKISKESEDFIVEQYKRLRQRDGSGVTKSSWRITVRQLESMIRLSES MARMHCCDEVQPKHVKEAFRLLNKSIIRVETPDVNLDQEEEIQMETDEGQGGVNGHADSPAPVNRFNGSS EDASQETVSKPSLRLGFAEYCRISNLIVLHLRKMEEEEDESALKRSELVNWYLKEIESEIDSEEELINKK TIIEKVVHRLTHYDHVLIELTQAGLKGSSEGSESYEEDPYLVVNPNYLLED TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008567 |
| ORF Size | 2463 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008567.2, NP_032593.1 |
| RefSeq Size | 2901 bp |
| RefSeq ORF | 2466 bp |
| Locus ID | 17219 |
| UniProt ID | P97311 |
| Gene Summary | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221835 | Mcm6 (untagged) - Mouse minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae) (Mcm6), (10ug) |
CNY 10,550.00 |
|
| MR210820 | Mcm6 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae) (Mcm6) |
CNY 6,024.00 |
|
| MR210820L3 | Lenti ORF clone of Mcm6 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae) (Mcm6) |
CNY 9,120.00 |
|
| MR210820L4 | Lenti ORF clone of Mcm6 (mGFP-tagged) - Mouse minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae) (Mcm6) |
CNY 9,120.00 |
