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Rapgef3 (NM_144850) Mouse Tagged ORF Clone
CAT＃: MG211040
- TrueORF®
Rapgef3 (tGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3)
ORF Plasmid: DDK
"NM_144850" in other vectors (3)
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CNY 8,930.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2310016P22Rik; 9330170P05Rik; Epac; Epac1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211040 representing NM_144850
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTTGAAGAGAATGCACCGTCCCCGCTGCTGCTCTTACCAGCTAGTGTTCGAGCACCGGCGCCCCA GCTGCATCCAGGGACTCCGCTGGACACCACTTACCAACAGCGAGGACTCCCTGGATTTCAGAGTGAGTCT GGAGCAGGCCACCACAGAGCATGTGCACAAGGCAGGGAAGCTCCTGCACCGCCATCTCTTGGCCACGTAC CCTACCCTCATCCGAGACAGAAAATACCATCTGCGACTATATCGGCATTGCTGCTCTGGCCGGGAGCTAG TGGATGGGATCTTGGCTCTGGGGCTTGGGGTCCACTCACGGAGCCAAGCCGTGGGCATCTGCCAAGTGTT GCTGGATGAGGGTGCCCTTTGTCATGTAAAACATGACTGGACCTTCCAGGACCGAGATGCCCAATTCTAC AGGTTCCCTGGACCGGAGCCCGAGCCTACAGGAACTCAAGATGTGGAAGAGGAGCTTGTTGAGGCTATGG CCCTCCTGTCCCAGCGAGGGCCTGATGCCCTACTCACCGTGGCGCTCCGGAAGCCCCCAGGTCAGCGTAC GGATGAAGAACTGGACCTGATCTTTGAAGAGCTGCTGCATATCAAGGCGGTGGCACACCTTTCTAACTCG GTGAAGCGGGAACTAGCTGCTGTTCTGCTCTTTGAACCACACAGCAAGGCAGGAACTGTGTTGTTCAGCC AGGGGGACAAGGGTACCTCGTGGTACATTATCTGGAAGGGATCTGTCAATGTGGTGACCCATGGCAAGGG GCTGGTGACCACGTTGCACGAGGGAGATGACTTTGGACAGCTGGCTCTGGTGAACGACGCACCTCGGGCA GCCACCATCATCCTTCGAGAAAATAACTGTCACTTTCTGCGTGTGGACAAGCAGGACTTCAACCGCATCA TCAAGGATGTGGAAGCAAAAACCATGAGACTGGAAGAACACGGCAAAGTGGTCTTAGTTCTGGAGAGAAC CTCTCAGGGTGCTGGCCCTTCCCGTCCCCCGACCCCAGGCAGGAACCGGTATACGGTCATGTCTGGCACC CCAGAGAAAATCCTAGAACTGCTGTTGGAGGCTATGAGACCGGATTCCAGTGCTCATGACCCAACAGAGA CGTTCCTCAGTGACTTCCTGCTGACCCACAGTGTCTTCATGCCCAGCACCCAGCTCTTCACTGCCCTCCT GCACCACTTCCACGTGGAGCCAGCAGACCCTGCTGGAGGCAGCGAGCAGGAGCACAGCACCTACATCTGC AACAAGAGGCAGCAGATCCTGCGGCTAGTTGGCCGATGGGTGGCCCTGTATAGCCCGATGCTCCACTCGG ATCCCGTGGCCACCAGCTTCCTCCAGAAACTCTCAGACCTGGTGAGCAGAGATGCCCGACTTAGCAACTT GCTGAGGGAACAGTATCCAGAGAGACGGCGACACCACAGGTTGGAAAATGGCTGTGGGAACGTATCTCCT CAGACCAAGGCCCGGAATGCACCTGTTTGGCTCCCTAACCAGGAGGAACCCCTCCCAAGCAGCGCGGGTG CCATCCGAGTTGGGGACAAAGTCCCCTACGACATCTGCAGACCTGACCACTCAGTGCTGACCCTGCACCT GCCGGTGACGGCCTCGGTGAGAGAAGTGATGGCAGCTTTGGCCCATGAGGACCACTGGACCAAGGGACAG GTGTTGGTGAAGGTCAATTCTGCCGGTGATGTCGTTGGCTTGCAGCCAGATGCCCGTGGTGTGGCCACAT CCCTGGGGCTCAACGAGCGGCTCTTTGTTGTCGACCCACAGGAAGTGCATGAGCTGACCCCACACCCTGA GCAGCTGGGCCCCACTCTGGGTTCTTCTGAGATGCTGGACCTAGTGAGCGCCAAGGACCTGGCAGGCCAG CTGACCGACCATGACTGGAACCTCTTCAACAGGATCCACCAGGTGCAGGAGCACCTGCGGGATGTCACCA CCGCGAACCTGGAGCGCTTCATGCGCCGCTTCAACGAGCTGCAGTACTGGGTGGCCACAGAGCTCTGTCT CTGCCCTGTTCCTGGCTCCCGGGCTCAGCTACTCAGGAAGTTCATCAAGCTGGCAGCCCACCTCAAGGAG CAGAAGAACCTCAACTCTTTCTTTGCGGTCATGTTTGGCCTCAGCAACTCGGCCATCAGCCGGCTGGCCC ACACCTGGGAGCGCCTGCCCCATAAAGTACGGAAGCTGTACTCAGCCCTGGAAAGGTTGCTGGACCCTTC CTGGAACCACCGAGTGTACCGATTGGCTCTCACCAAGCTCTCCCCTCCTGTCATCCCCTTCATGCCCCTG CTGCTCAAAGACGTGACCTTCATCCATGAGGGGAACCACACACTGGTAGAGAACCTCATCAACTTTGAGA AGATGCGGATGATGGCCAGAGCGGTGCGGATGCTTCACCACTGCCGAAGTCACAGTACCGCGCCTCTATC ACCACTCAGAAGCCGAGTGTCCCACATCCACGAGGACAGCCAGGGATCAAGGATCTCCACGTGTTCTGAG CAGTCCCTGAGCACCCGGAGTCCAGCCAGCACCTGGGCTTATGTCCAGCAGCTGAAGGTCATTGACAACC AGCGGGAACTGTCCCGCCTCTCCCGGGAGCTGGAACCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211040 representing NM_144850
Red=Cloning site Green=Tags(s) MVLKRMHRPRCCSYQLVFEHRRPSCIQGLRWTPLTNSEDSLDFRVSLEQATTEHVHKAGKLLHRHLLATY PTLIRDRKYHLRLYRHCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALCHVKHDWTFQDRDAQFY RFPGPEPEPTGTQDVEEELVEAMALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNS VKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRA ATIILRENNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGAGPSRPPTPGRNRYTVMSGT PEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPSTQLFTALLHHFHVEPADPAGGSEQEHSTYIC NKRQQILRLVGRWVALYSPMLHSDPVATSFLQKLSDLVSRDARLSNLLREQYPERRRHHRLENGCGNVSP QTKARNAPVWLPNQEEPLPSSAGAIRVGDKVPYDICRPDHSVLTLHLPVTASVREVMAALAHEDHWTKGQ VLVKVNSAGDVVGLQPDARGVATSLGLNERLFVVDPQEVHELTPHPEQLGPTLGSSEMLDLVSAKDLAGQ LTDHDWNLFNRIHQVQEHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGSRAQLLRKFIKLAAHLKE QKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSPPVIPFMPL LLKDVTFIHEGNHTLVENLINFEKMRMMARAVRMLHHCRSHSTAPLSPLRSRVSHIHEDSQGSRISTCSE QSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_144850 |
| ORF Size | 2628 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144850.1, NP_659099.1 |
| RefSeq Size | 3644 bp |
| RefSeq ORF | 2757 bp |
| Locus ID | 223864 |
| UniProt ID | Q8VCC8 |
| Gene Summary | Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR211040 | Rapgef3 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), transcript variant 2 |
CNY 6,424.00 |
|
| MR211040L3 | Lenti ORF clone of Rapgef3 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), transcript variant 2 |
CNY 9,980.00 |
|
| MR211040L4 | Lenti ORF clone of Rapgef3 (mGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), transcript variant 2 |
CNY 9,980.00 |
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