Als2 (BC031479) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Als2cr6, MGC27807
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211215 representing BC031479 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCAAAGAAGAAAAGCTCAACAGAGGCAGAAGGATCCAAAGAAAGAGGCCTAGTCCATGTCTGGC AGGCAGGATCCTTTTCTCTAACACCAGAGAGGTTGCCAGGCTGGGGTGGAAAGACAGTTCTTCAGGCAGC CCTTGGTGTGAGGCATGGAGTTCTTCTGACTGAAGATGGTGAGGTCTACAGCTTTGGGACTCTTCCCTGG AAAAGTGAATCAGCAGAAATTTGTCCAAGCAGCCCCCTTCTAGAAAGTGCCCTGGTTGGGCATCATGTTA TTACTGTGGCAACAGGGAGCTTCCACAGTGGAGCAGTGACAGAGAGCGGGGTGGTGTACATGTGGGGAGA GAATGCTGCCGGGCAGTGTGCGGTAGCTAACCAGCAGTATGTGCCGGAGCCGAGTCCTGTCAGCATTTCT GACTCGGAGACCAGCCCGTCATTAGCAGTTAGGATTCTGCAATTGGCATGTGGCGAGGAACACACACTGG CATTGTCACTCAGCAGAGAGATCTGGGCATGGGGCACCGGCTGTCAGCTGGGCCTCATCACCACCACTTT CCCAGTGACAAAGCCACAGAAGGTGGAACACCTTGCTGGACGAGTGGTGCTCCAGGTGGCCTGCGGTGCA TTCCACAGCCTTGCACTTGTGCAGTGCCTCCCTCCTCAGGATCTGAAGCCAGTCCCAGAGAGATGCAATC AGTGCAGCCAGCTGCTCATCACCATGACAGACAAAGAGGACCATGTGATAATATCGGACAGCCATTGCTG CCCTTTAGGTGTGACATTGTCCGAGTCTCAAGCAGAAAAGCATGCCAGCCCTGCTCCCAGCCCTCACCCA GAGGCACTGGATGAGCAGGGAGAGGTGTTTGAGAACACGGTGGTAGAAGCTGAACTGAACATGGGAAGCA GTCAGACCACAAGTGGCAGTGCCATTTCCACCCAGCAGAACATCGTGGGAACAGCTGAAGTGTCTTCTGC CAGAACAGCTCCGTCATACCCAGACACCCATGCGGTAACTGCATACCTGCAGAAGCTGTCAGAGCATTCG ATGAGGGAGAACCATGAGCCTGGAGAAAAGCCACCCCAGGTCCAGCCTCTTGTAGAAGAAGCAGTTCCTG ATCTTCACAGTCCACCAACCACAAGCACCTCAGCCCTCAACAGCTTGGTGGTCTCCTGTGCATCTGCTGT TGGTGTCAGAGTGGCTGCCACCTATGAAGCTGGGGCCTTGTCTCTCAAGAAAGTTATGAACTTTTACAGC ACTGCCCCCTGCGAGACGGCAGCTCAGTCGGGCAGTGCCTCCACAGGCCCAGAAAGTCTGAAAGATCTCC GAGAAGAGCAGGTGAAACAGGAGTCACTGCAAGGGAAGAAAAGCTCAAGTCTCATGGACATCAGAGAGGA AGAGTCGGAGGGAGGGAGTCGAAGACTCTCCCTCCCAGGGTTGTTGTCGCAAGTTTCCCCCAGGCTCTTA AGGAAGGCTGCGAGGGTGAAAACTCGGACAGTGGTTCTGACTCCCACATACAGTGGAGAAGCAGATGCCC TTCTGCCTTCCCTGAGGACAGAGGTGTGGACCTGGGGGAAAGGCAAGGAAGGGCAGCTAGGGCACGGCGA CGTCCTGCCCAGGCTTCAGCCGTTGTGTGTCAAGTGTCTGGATGGTAAAGAGGTAATCCACCTGGAGGCG GGCGGCTCCCACTCCCTCGCACTCACTGCGAAATCTCAGGTTTACTCATGGGGCAGTAATACCTTTGGTC AGCTTGGGCATTCTGAGTTTCCAACAACGGTTCCTCGACTCTCAAAGGTTAGCAGTGAAAATGGAGTCTG GAGTGTAGCTGCAGGCCAAGATTATTCCTTGTTTTTAGTGGACACGGAAGACTTCCAGCCTGGGTTGTAT TACAGTGGCCGACAGGACCGTGCAGAAGGTGATACCCTGCCAGAGAATCCCAGTGGTACAAAGACTCCAG TACTTCTCTCCTGTAGTAAGCTTGGATACATAAGCAGAGTAACAGCAGGAAAAGATAGCTATCTAGCCTT GGTGGATAAGAACATCATGGGATACATCGCCAGTCTCCATGAGTTGGCTTCTACAGAAAGACGGTTTTAC TCAAAACTGAGCGAAATCAAATCACAGATACTTAGGCCTCTTCTCAGTTTAGAAAATTTGGGCACAGTGA CCACTGTCCAGCTGTTGCAGGAAGTTGCCAGCCGGTTCAGCAAGTTGTGTTACCTCATTGGGCAGCATGG AGCCTCACTAAGCAGCTACCTACAGGGTATGAAGGAAGCCAGCAGCCTGGTCATCATGAAGCACTCAAGT CTTTTCCTGGACAGCTACACAGAGTACTGCACATCAGTTTCAAATTTCCTGGTTATGGGAGGATTCCAGC TTCTTGCTAAGCCTGCCATTGATTTCCTAAATAAAAACCAAGAACTCTTGCAAGATTTGTCAGAAGTGAA TGATGAGAACACTCAGTTGATGGAAATCCTGAACATGCTGTTTTTCTTGCCAATCAGACGACTTCATAAT TATGCAAAAGTTTTGCTAAAGCTTGCCACTTGCTTTGAAGTGGTAGGTTTCGTGTGTGCCCCTCCAAATA CTCGGGAAGCCAAGAGTCAAAGTAGTTTGTTTGCATTAAGTCTCCTTAAGATGTTGGGCCCCGGGGCTGG TGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAAGGTCTGGAGTTCAAATCCCAGCAACCA CATGGTGGCTCACAACCACCCGTAATGAGATCTGATGCCCTCTTCTGGAGTGTC ACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211215 representing BC031479 Red=Cloning site Green=Tags(s) MDSKKKSSTEAEGSKERGLVHVWQAGSFSLTPERLPGWGGKTVLQAALGVRHGVLLTEDGEVYSFGTLPW KSESAEICPSSPLLESALVGHHVITVATGSFHSGAVTESGVVYMWGENAAGQCAVANQQYVPEPSPVSIS DSETSPSLAVRILQLACGEEHTLALSLSREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGA FHSLALVQCLPPQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEKHASPAPSPHP EALDEQGEVFENTVVEAELNMGSSQTTSGSAISTQQNIVGTAEVSSARTAPSYPDTHAVTAYLQKLSEHS MRENHEPGEKPPQVQPLVEEAVPDLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYS TAPCETAAQSGSASTGPESLKDLREEQVKQESLQGKKSSSLMDIREEESEGGSRRLSLPGLLSQVSPRLL RKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEA GGSHSLALTAKSQVYSWGSNTFGQLGHSEFPTTVPRLSKVSSENGVWSVAAGQDYSLFLVDTEDFQPGLY YSGRQDRAEGDTLPENPSGTKTPVLLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELASTERRFY SKLSEIKSQILRPLLSLENLGTVTTVQLLQEVASRFSKLCYLIGQHGASLSSYLQGMKEASSLVIMKHSS LFLDSYTEYCTSVSNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVNDENTQLMEILNMLFFLPIRRLHN YAKVLLKLATCFEVVGFVCAPPNTREAKSQSSLFALSLLKMLGPGAGEMAQWVRAPDCSSEGLEFKSQQP HGGSQPPVMRSDALFWSV TRPLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	BC031479
ORF Size	2784 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC031479, AAH31479
RefSeq Size	2955 bp
RefSeq ORF	2786 bp
Locus ID	74018
MW	100.1 kDa
Gene Summary	May act as a GTPase regulator. Controls survival and growth of spinal motoneurons.[UniProtKB/Swiss-Prot Function]

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