Pcdhgb6 (BC054556) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211226 representing BC054556 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGGGAGCAGCGCGCGGAGGAAGCGCCCCGACCGCCCGCAGGTACTGTTTACCCTGCTGCTGCCTT TGTTCTGTCCCGCGCTGGGCCAGCCGGTCCGCTACTCCATCCCCGAGGAGCTGGACCGCGGATCGGTGGT GGGGAAGCTCGCCAAGGACCTGGGGCTCAGTGTCCTGGAAGTGTCGTCTCGGAAGCTGCGGGTCAGCGCT GAGAAGCTGCACTTCAGCGTGGATTCGGAGAGCGGGGACTTACTGGTGAAGGACCGCATAGATCGCGAGC AGATATGCAAAGGGAGGAGAAAGTGTGAGCTGCAGTTGGAAGCTGTGCTGGAAAACCCTTTAAATATTTT TCATGTCGTTGTGGGAATTGAGGATGTTAATGATAATGCTCCTCAATTCGAGAAGAAAGAAACGCGTTTA GAAATCTTAGAAACTGTGGCGGTTGGTACACGAATACCCCTTGAGCCTGCCACTGACCCCGACATAAATT TGAATTCGGTTAAAGATTACCAGATAAGCTCCAACCCTTATTTCTCATTAATGGTTAGAGTTAATCCTGA TGGTGGCAAAACCCCAGAGTTGTCTCTGGAGAAACTCCTAGATAGGGAAGAACAGAGGTCTCATCGCTTG ATACTGACTGCCTTGGATGGAGGGGACCCACCCCGGAGTGCCACCACTCAGATAGAAATATCTGTAAAGG ACAACAATGACAACCCTCCAGTGTTTAGCAAAGAGGAATATTGGGTCAGTGTTAGTGAAAATCTGTCCCC AGGATCCTCTGTGTTGCAAGTGACAGCCACTGATGAGGATGAGGGCGTCAATGCTGAGATACTTTACTAC TTTCGGAGCACTGCGCAGAGCACAAGACACGTGTTCTCACTAGATGAGAAAACAGGCGTGATCAAGAATA ACCAGTCTCTGGATTTTGAAGACATAGAGAGGTACACCATGGAAGTAGAAGCGAAGGACGGCGGTGGTCT CTCTACACGGTGTAAAATCATCATAGAAGTCCTAGATGAAAATGACAACAGTCCAGAAATCACCATCACC TCCCTTTCTGATCATATCTTGGAGAATTCTCCTCCGGGAGTGGTGGTTGTCCTCTTCAAAACGAGAGATC GGGATTTCGGAGGAAATGGGGAAGTCACGTGTGATATAGGCAAAGACCTGCCTTTCAAGATCCAAGCTTC TTCTAGCAACTACTACAAGTTAGTAACAGATGGAGCCCTAGACCGAGAGCAGAACCCACAGTACAATGTC ACCATCACCGCCACGGACAAAGGCAAGCCAGCACTCTCTTCCAGTACAACCATCGTTCTGCACATCACTG ACATTAACGACAACGCTCCGGCTTTCCAGAAATCTTCATACATAGTCCATGTGGCAGAGAACAATCCGCC TGGAGCCTCCATCGCTCAAGTCAGCGCCTCCGACCCGGATCTGGGGGCCAATGGTCATGTCTCCTACTCC ATCATAGCCAGTGACCTGGAGCCTAAATCGCTGTGGTCCTACGTGACCGTGAATGCGCAGAGCGGAGTGG TGTTCGCACAGCGCGCCTTCGACCACGAACAGCTGCGCTCCTTCCAGCTGACACTGCAGGCGCGCGACCA GGGAAAGCCCTCGCTCAGCGCCAACGTGAGCATGCGCGTGTTGGTGGGTGACCGCAACGACAACGCGCCA CGCGTGCTGTATCCCGCGCTCGAGCCCGACGGCTCTGCGCTCTTCGACATGGTGCCACGCGCTGCCGAGC CTGGATACCTGGTAACCAAAGTGGTGGCGGTGGACGCAGACTCAGGACACAATGCCTGGCTGTCTTACCA CGTGCTGCAGGCCAGCGATCCCGGGCTCTTCAGCCTGGGACTGCGCACAGGCGAGGTGCGCACAGCGCGT GCCTTGGGCGAAAAGGATGCTGCGCGGCAGCGCCTGTTGGTTGGTGTTCGCGATGGTGGACAGCCACCCC TCTCGGCCACCGCCACGCTGCTTCTAGTATTCGCTGATAGCTTGCAGGAGGCCCTGCCAGACCTCAGTGA CCGCTCACTGCCCCCTGATCCGCAAGCCGAGCTGCAGTTCTACTTGGTGGTGGCCTTGGCCTTGATTTCT GTGCTTTTCCTCCTGGCTGTGATTCTAGCCATTGCACTTCGACTGCGACACTCTTCCAGCCCCTCGGTCT GGGGCTGCTTTCAGTCTGGTCTTTGTTCTAAGGCTAGACCAGGGGTTTCTCTCAACTATAGCGAGGGCAC TCTACCCTATTCCTATAATCTGTGCGTTGGGCAAACGGGAAAGGCGGAGTTAAATTTTCTGAAATGTAGC GCACCTTTGCCTTCTTCTCAAGAGATCGTTTCCGGTAGTTCTCCTGGGGCCTTAATTCCGCTTCACCTTG GAGATGATTTGACAACACATCCTGAGACTCTAACACCGCAAGCCCCGCCCAACACTGACTGGCGTTTCTC TCAAGCCCAGAGACCCGGCACGAGCGGATCCCAAAATGGTGATGAAACTGGCACCTGGCCCAACAACCAG TTTGATACAGAGATGCTGCAAGCCATGATCTTGGCCTCTGCCAGCGAAGCTGCTGATGGGAGCTCTACCC TGGGAGGGGGCGCTGGCACCATGGGTCTGAGCGCTCGATACGGACCCCAGTTTACCCTGCAGCACGTGCC TGACTACCGCCAGAACGTGTACATCCCTGGCAGCAATGCCACGCTGACCAATGCCGCTGGCAAACGAGAT GGCAAGGCTCCAGCAGGTGGTAATGGCAACAAGAAGAAATCGGGCAAGAAAGAGAAGAAG ACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211226 representing BC054556 Red=Cloning site Green=Tags(s) MGGSSARRKRPDRPQVLFTLLLPLFCPALGQPVRYSIPEELDRGSVVGKLAKDLGLSVLEVSSRKLRVSA EKLHFSVDSESGDLLVKDRIDREQICKGRRKCELQLEAVLENPLNIFHVVVGIEDVNDNAPQFEKKETRL EILETVAVGTRIPLEPATDPDINLNSVKDYQISSNPYFSLMVRVNPDGGKTPELSLEKLLDREEQRSHRL ILTALDGGDPPRSATTQIEISVKDNNDNPPVFSKEEYWVSVSENLSPGSSVLQVTATDEDEGVNAEILYY FRSTAQSTRHVFSLDEKTGVIKNNQSLDFEDIERYTMEVEAKDGGGLSTRCKIIIEVLDENDNSPEITIT SLSDHILENSPPGVVVVLFKTRDRDFGGNGEVTCDIGKDLPFKIQASSSNYYKLVTDGALDREQNPQYNV TITATDKGKPALSSSTTIVLHITDINDNAPAFQKSSYIVHVAENNPPGASIAQVSASDPDLGANGHVSYS IIASDLEPKSLWSYVTVNAQSGVVFAQRAFDHEQLRSFQLTLQARDQGKPSLSANVSMRVLVGDRNDNAP RVLYPALEPDGSALFDMVPRAAEPGYLVTKVVAVDADSGHNAWLSYHVLQASDPGLFSLGLRTGEVRTAR ALGEKDAARQRLLVGVRDGGQPPLSATATLLLVFADSLQEALPDLSDRSLPPDPQAELQFYLVVALALIS VLFLLAVILAIALRLRHSSSPSVWGCFQSGLCSKARPGVSLNYSEGTLPYSYNLCVGQTGKAELNFLKCS APLPSSQEIVSGSSPGALIPLHLGDDLTTHPETLTPQAPPNTDWRFSQAQRPGTSGSQNGDETGTWPNNQ FDTEMLQAMILASASEAADGSSTLGGGAGTMGLSARYGPQFTLQHVPDYRQNVYIPGSNATLTNAAGKRD GKAPAGGNGNKKKSGKKEKK TRPLE - GFP Tag - V
Restriction Sites	SgfI-NotI      Cloning Scheme for this gene
ACCN	BC054556
ORF Size	2790 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC054556, AAH54556
RefSeq Size	4773 bp
RefSeq ORF	2792 bp
Locus ID	93703

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