Efcab3 (BC056955) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	bM150J22.2, mKIAA1672, 4931407K02Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211311 representing BC056955 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGTGTATTCTGCCCATATCTATCAAATGAGCACTTAGTAAGATTCTCCAGTAAGTTCCAAGAAGCCG GCTCAGGAAGGATACTGTACAAGAAATTTCTGTTATCCATTGGTGTGGGTATCCCACCACCTACACCCCC GCTGTCTTCTCCGAAGGTTCAGCTAAGTGACCAGTTTCAAATTGAGGAGCCGGGCCAGCAGGATGAGAGA ACCCAGCCCTCCGGGGAAAAAACTTCGGAGATCAACAACATGACCAAGGAAGAAGTCATTGACGGTCTCA AGCACCGGATCCAGCAGAAGGACCCAGTATTCAGAAAGCAGTTTCTTAGCATCAGCAAGGAACCTGATGT AAAGATCAACCAAGAGGAATTCCGGAAGGTCCTAGAGAGAAGTGGAATGCCTATGAATGACTGCCAGTAT GCGATGCTGGCTTCCAAGTTAGGCTTCAAGAACGAAGAAGGCATGAGCTATCAGGACTTCACCATGGGAT TTGAAGACTGTATGCTGAGCGGACTGGAAACTGTCCCATTGCAGTCCCGCACGGCCTCAAGAACGAACAT GGATGAGCACTTCATATCTGCCGAGGAATGCCTGAGAATTTTCCCTAAGAAACTGAAGGAATCCTTCCGG GACGTGTACTCCGCCTTCTTTAGAATAGACTTGGACAGGGATGGCATTATAAGCATGCACGACTTTCACA GACTGCTGCAGTACCTACAGCTCAACATGGTCGACCTCGAGTTTGAGCGCTTCCTCAGCCTCCTGGGCCT GAGACTTAGTGTCACTCTAAACTTCCGAGAGTTCCAGAACCTGTGTGAGAAGAGGCCCTGGAAATCAGAT GAGGCACCTCAAAGGCTCATCAGATGTAAACAGAAAGTTGCAGATTCAGAGCTAGCCTGTGAGCAGGCTC ATCAGTACCTCATCATGAAAGCCAAAACCAGATGGGCAGACCTGTCCAAGAATTTCATAGAAACGGATAA TGAGGGCAACGGCATCCTCCGGCGAAGGGACATCAAGAACTCGCTCTATGGCTTTGACATCCCCCTCACC CCAAGAGAATTTGAGAAGCTTTGGCAAAACTATGACACCGAGGGAAGGGGTTATATCACGTACCAAGAGT TCCTGCACAGACTGGGCATTCGATACTCCCCGAAGGTCCACCGGCCCTACAAGGAAGACTACTTCAACTT CCTGGGTCACTTCACGAAGCCCAAGCAGGTCCAGGAGGAAATCCAGGAGCTACAGCAGATCAGTGAGAGG GAGAAACTCATGAACCATTATGAGGAGATCAGCAAGGCGTTCAACGCAATGGAAAAATCCAAGCCCGTCG CCCTTTGCAGGGTGCAGAAGGTGCTACAGGAATGTGGGTGTCCCTTGAAGGAGGAGGAGCTCATCAGCCT TCTGAAGAGCTTGGACGTCAGTGTGCATAACAATCACATCGACCCTGTCGAATTCCTGAGAGCGCTTGAG ATCAGCTGGGCCAGCAAAGCTCGCCCAAAGGAGAAGGAGGAAAGCTCGCCACCGCCCATCAGCTTCTCTA AGGTGACCCCAGATGAGGTGATAAAGACCATGCAGGAGGTGGTGGAGTCCTCCCAGCCTGCTTTGGTGGA GGCCTTCTCTGCACTGGATAAAGAGGACACAGGGTTCGTGAAGGCTATGGAATTTGGAGATGTCCTGCGG AGCGTATGCCAAAAGCTAACGGATAACCAATATCATTACTTCTTGAGGAGATTAAGGCTCCACCTAACAC CCAACATACACTGGAAATACTTCCTGGAGAACTTCAGCACCTTCCAGGACGAGACTGCTGACGACTGGGC AGAGAACATGCCCAAGGCCCCGCCCCCCATGTCCCCCAAAGAAACAGCCCACAGAGACATCGTGGCACGA GTGCAGAAGGCAGTAGCGTCTCACTACCACACGATTGTCCAGGAGTTTGAGAACTTCGACACCCTGAAAA GCAACACGGTTTCCAGAGACGAGTTCAGGTCCATCTGCACCCGCCACATACAGATCCTGACAGACGAGCA GTTTGACAGACTGTGGAGCGAGCTGCCTGTCAATGCCAAGGGGAGGCTAAAGTACCAGGACTTCCTCAGC AAGTTGAGCATCGAGAGGGTGCCCTCGCCGCCCATGGCCGCAGGCGACTCTGGAGAATCGACCATGGCCC AGCGGGGAAGCAGCGCCCCTGAGTTCTCCCAAGGGACCAGGTCCAACCTCTACTCGCCACCTCGGGACTC CAGAGTGGGGTTGAAATCACGGAGCCACCCCTGTACCCCAGTGGGCACCCCACCCCTACAGAACTGCGAG CCCATCGAGAGCCGGCTCCGCAAACAGATCCAGGGCTGCTGGCGGGAGCTGCTGAGGGAGTGCAAGGAGA AAGACACGGACAAGCAGGGGACCATCTCTGCAGCTGAGTTCCTGGCGCTCGTGGAGAAGTTCAAGCTGGA CATCAGCAGGGAGGAGAGCCAGCAGCTCATCGTCAAGTACGACCTGAAGAACGGCAAGTTCGCCTACTGC GACTTCATCCAGAGCTGCGTCCTGCTGCTGAAGGCCAAGGAGACCTCTCTGATGCGGAGGATGAGGATCC AGAATGCGGACAAGATGAAAGAAGCTGGCATGGAGACACCTTCCTTTTACTCGGCCTTGCTACGGATCCA GCCTAAGATCGTTCACTGCTGGAGGCCCATGCGGCGTTCCTTCAAGACCTACGACAAGAACGGAACGGGA CTCCTGAGTGTGGCTGACTTCAGGAAGGTGCTGCGGCAGTACAGCATTAATCTGTCAGAGGAGGAGTTCT TCCACGTGCTCGAGTACTATGACAAATCACTGTCTTCGAAGATTTCCTACAATGACTTCCTGCGTGCCTT CCTGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211311 representing BC056955 Red=Cloning site Green=Tags(s) MRVFCPYLSNEHLVRFSSKFQEAGSGRILYKKFLLSIGVGIPPPTPPLSSPKVQLSDQFQIEEPGQQDER TQPSGEKTSEINNMTKEEVIDGLKHRIQQKDPVFRKQFLSISKEPDVKINQEEFRKVLERSGMPMNDCQY AMLASKLGFKNEEGMSYQDFTMGFEDCMLSGLETVPLQSRTASRTNMDEHFISAEECLRIFPKKLKESFR DVYSAFFRIDLDRDGIISMHDFHRLLQYLQLNMVDLEFERFLSLLGLRLSVTLNFREFQNLCEKRPWKSD EAPQRLIRCKQKVADSELACEQAHQYLIMKAKTRWADLSKNFIETDNEGNGILRRRDIKNSLYGFDIPLT PREFEKLWQNYDTEGRGYITYQEFLHRLGIRYSPKVHRPYKEDYFNFLGHFTKPKQVQEEIQELQQISER EKLMNHYEEISKAFNAMEKSKPVALCRVQKVLQECGCPLKEEELISLLKSLDVSVHNNHIDPVEFLRALE ISWASKARPKEKEESSPPPISFSKVTPDEVIKTMQEVVESSQPALVEAFSALDKEDTGFVKAMEFGDVLR SVCQKLTDNQYHYFLRRLRLHLTPNIHWKYFLENFSTFQDETADDWAENMPKAPPPMSPKETAHRDIVAR VQKAVASHYHTIVQEFENFDTLKSNTVSRDEFRSICTRHIQILTDEQFDRLWSELPVNAKGRLKYQDFLS KLSIERVPSPPMAAGDSGESTMAQRGSSAPEFSQGTRSNLYSPPRDSRVGLKSRSHPCTPVGTPPLQNCE PIESRLRKQIQGCWRELLRECKEKDTDKQGTISAAEFLALVEKFKLDISREESQQLIVKYDLKNGKFAYC DFIQSCVLLLKAKETSLMRRMRIQNADKMKEAGMETPSFYSALLRIQPKIVHCWRPMRRSFKTYDKNGTG LLSVADFRKVLRQYSINLSEEEFFHVLEYYDKSLSSKISYNDFLRAFLQ TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC056955
ORF Size	2877 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC056955, AAH56955
RefSeq Size	3433 bp
RefSeq ORF	2879 bp
Locus ID	77627
Gene Summary	Negatively regulates the androgen receptor by recruiting histone deacetylase complex, and protein DJ-1 antagonizes this inhibition by abrogation of this complex.[UniProtKB/Swiss-Prot Function]

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