Ahi1 (BC055400) Mouse Tagged ORF Clone
CAT＃: MG211435
- TrueORF®
Ahi1 (tGFP-tagged) - Mouse Abelson helper integration site (cDNA clone MGC:62786 IMAGE:6467369)
ORF Plasmid: DDK
"BC055400" in other vectors (4)
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CNY 11120.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1700015F03Rik; Ahi-1; D10Bwg0629e |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211435 representing BC055400
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCAGAAACTCCAGAGAAGGTGGATTCTGCACAAGAGAAAGTCAGAGGCAAGACCCCGACAGCAG ATGATTCAGATGACAGCCGAGAAAAGACTGGCATAGAAGAGAAAGGAGAACTGACCGACGCCTATCAGCT GCAGGTAGCTGAAGAAATGGCAAAGGAGATCAAGAAGAAAATAAGAAAGAAGCTAAAGGAACAGCTGACC TACTTTCCTCCAGACACTCTATTGCATGACGACAAGCTGGCCAGTGAAAAAAGAAAGAAGAAGAAGAAGA AAGTGCCAGTGCCCACTAAGCCTGAGTCAAGTCCCTCAGATGTCTGTGACAGTGCAGTTGAAGGGGAACA AAAGAAAGAAGGTACCCCTGAGGACTCTCAGCACATGGAGGGAATCTGCTCGAGAGAGCAGGATGTGGAT GCCACTGTGCCAGAGAACGCAAAGCCCAAACCCAAGAAGACAAAGAAGAAGACTAAAGCAGTTTCAAATG ATAATGAAGACACTAATGGAGATGGTGTTCATGAGATAACAAGCCGAGACAGCCCAGTTCATCCCAAGTG CTTGCTGGATGATGACCTCGTCATGGGAGTCTACATTCACCGAACCGATAGACTTAAATCTGACTTTATG ATTTCTCACCCAATGGTAAAGATCCATGTGGTTGATGAGCACACTGGCCAGTACGTCAAGAAAGATGACA GTGAACGTCCTGTTTCATCTTATTATGAGAAAGACAACGTGGACTATATTCTCCCTATTATGACACAGCC ATATGACTTTAAAAAGCTAAAGTCAAGGCTTCCGGAGTGGGAGGAGCAAGTTATTTTTAATGAAAATTTT CCCTATTTGCTTCGAGAGTTTGAAGAATGTCCAAAAGTCATCCTGTTCTTTGAGATTCTTGACTTTTTAA GCATGGATGAAATCAAGAATAACTCTGAGGTTCAAAACCAAGAGTGTGGCTTTCGGAAAATTGCCTGGGC GTTTCTTAAGCTTCTGGGAGCCAATGGGAATGCAAACATCAACTCAAAACTTCGCCTGCAGCTCTACTAC CCACCGACTAAGCCTCGATCCCAGCTAAATGTCGTCGAGGTTTTTGAATGGTGGTCCAAGTGTCCAAGAA ATCGTTATCCATCAACATTGTATGTAACCGTACGAGGATTGAAAGTCCCGGACTGTATAAAGCCATCTTA CCGCTCTATGATGGCTCTCCAGGAGGAAAGGGGTACACCAGTGTACTGTGAACGTCACCGTGAAACAAGT TCCGTGGACACAGAACCTGGACTAGAAGATTCAAAGGAGGAAGTGAAGTGGAAACGTCTGCCTGGCCAGG CCTGTCGTATCCCAAACAAGCATCTCTTCTCACTGAATGCTGGAGAACGCGGCTGTTTCTGTCTTGATTT CTCTCACAATGGAAGGATATTAGCAGCAGCCTGTGCCAGCCGAGACGGATATCCGATTATATTATATGAA ATTCCTTCTGGACGTTTTATGAGAGAATTGTGTGGCCACCTCAATATCATTTATGATCTCGACTGGTCAA AAGACGATCGCTATCTCGTTACTTCATCCTCTGACGGCACTGCCAGAGTTTGGAAGAATGAAATCAATAG CACGAGCACGTTCAGAGTCTTACCTCACCCCTCCTTCGTCTACACGGCTAAATTCCACCCAGCCACACGG GAGCTGGTGGTTACAGGATGCTACGACTCTATGATAAGGATTTGGAAAATTGATGCAAGGGAGGATGCTG CCATATTAGTGCGCCAGTTGGATGTGCACAAGAGCTTTGTCAACTCCATCTGTTTTGACGATGAAGGTCA CCACATGTACTCAGGAGACTGCATCGGGGTCATCGTTGTCTGGGACACGTACGTCAAAGTGAATGATGTG CAACACTCCGTGCGCCACTGGACGATAAATAAGGAAATTAAGGAAACTGAATTCAGGGGAGTTCCAATAA GTTACCTGGAGGTTCATCCCAATGGAAAGCGTTTGCTAATCCACACCAAAGACAGTACTCTGAGGATTAT GGACCTGCGGATATTGGCAGCCAGGAAATTTGTGGGTGCAGCAAATTACCGTGAGAAGATCCATAGCACC TTGACGCCGTGCGGGACTCTGCTCTTTTCTGGGAGTGAGGATGGGATAGTATACGTTTGGAACCCAGAGA CAGGAGAACAAGTGGCAATGTACTCTGACCTGCCATTCAAGTCCACAATCCGAGACATCTCTTACCACCC CCTGGAGAATATGGTTGCCTTCTGTGCCTTTGGACAGAGCGAGCCGATTCTTCTTTATATTTATGATTTC CAGGTAGCCCAGCAGGAGGCTGAAATGTTGAAACGCTATAGTGGGACACTCCCGTTGCCTGGGATACATC AGAGTGAGGATGCTTTATGCACCTGCCCTAAGCTGCCCCAGCAGGGCTCTTTTCAGATTGATGAATTTGT CAACACTGAAAATAGTTCATCAAGAAAGATTCAACTTGTGAAACAGAGACTCGAAACTGTCACAGAGGTG ATACGATCCTGTGCTGCAAAAGTCAACAAAAATCTCTCAATGACTTCGCCACCACCAGGCCCTGCAAAGA AGCCCAGGGTAAAGCAGTCGTTCGTGCTGACCACTGATGAGATCATACATCAGTTTGGACTCCCCCAGAC TGCATTTATCAGCATAGAGAGAGGGCCTTTTGTGCGCCATGTTGATCCACCACCAATGGTAGTGGCTCTT TATGACTACACAGCCAGCCGATCAGATGAACTAACCATCCATCGTGGAGACATTATCCGAGTGTATTTCA AAGATAATGAAGACTGGTGGTACGGCAGCGTAAGAAAGGGGCAGGAAGGGTTCTTTCCAGCTAATCATGT GGCCAGTGAAACACTGTATCGAGACTCCCCTCCGAAGGTAAAGGAGCGCTCCCCTCCTTTAACTCCCAAG GAGAAAACTAAACCAGAAAAGCCTCTGGCTTCTCAAAAGGGTGGCCATGAAGAGGAGACGAAGTCACAAA CCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211435 representing BC055400
Red=Cloning site Green=Tags(s) MEPETPEKVDSAQEKVRGKTPTADDSDDSREKTGIEEKGELTDAYQLQVAEEMAKEIKKKIRKKLKEQLT YFPPDTLLHDDKLASEKRKKKKKKVPVPTKPESSPSDVCDSAVEGEQKKEGTPEDSQHMEGICSREQDVD ATVPENAKPKPKKTKKKTKAVSNDNEDTNGDGVHEITSRDSPVHPKCLLDDDLVMGVYIHRTDRLKSDFM ISHPMVKIHVVDEHTGQYVKKDDSERPVSSYYEKDNVDYILPIMTQPYDFKKLKSRLPEWEEQVIFNENF PYLLREFEECPKVILFFEILDFLSMDEIKNNSEVQNQECGFRKIAWAFLKLLGANGNANINSKLRLQLYY PPTKPRSQLNVVEVFEWWSKCPRNRYPSTLYVTVRGLKVPDCIKPSYRSMMALQEERGTPVYCERHRETS SVDTEPGLEDSKEEVKWKRLPGQACRIPNKHLFSLNAGERGCFCLDFSHNGRILAAACASRDGYPIILYE IPSGRFMRELCGHLNIIYDLDWSKDDRYLVTSSSDGTARVWKNEINSTSTFRVLPHPSFVYTAKFHPATR ELVVTGCYDSMIRIWKIDAREDAAILVRQLDVHKSFVNSICFDDEGHHMYSGDCIGVIVVWDTYVKVNDV QHSVRHWTINKEIKETEFRGVPISYLEVHPNGKRLLIHTKDSTLRIMDLRILAARKFVGAANYREKIHST LTPCGTLLFSGSEDGIVYVWNPETGEQVAMYSDLPFKSTIRDISYHPLENMVAFCAFGQSEPILLYIYDF QVAQQEAEMLKRYSGTLPLPGIHQSEDALCTCPKLPQQGSFQIDEFVNTENSSSRKIQLVKQRLETVTEV IRSCAAKVNKNLSMTSPPPGPAKKPRVKQSFVLTTDEIIHQFGLPQTAFISIERGPFVRHVDPPPMVVAL YDYTASRSDELTIHRGDIIRVYFKDNEDWWYGSVRKGQEGFFPANHVASETLYRDSPPKVKERSPPLTPK EKTKPEKPLASQKGGHEEETKSQTN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC055400 |
| ORF Size | 3015 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC055400.1 |
| RefSeq Size | 4843 bp |
| RefSeq ORF | 3017 bp |
| Locus ID | 52906 |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223087 | Ahi1 (untagged) - Mouse Abelson helper integration site (cDNA clone MGC:62786 IMAGE:6467369), (10ug) |
CNY 12920.00 |
|
| MR211435 | Ahi1 (Myc-DDK-tagged) - Mouse Abelson helper integration site (cDNA clone MGC:62786 IMAGE:6467369) |
CNY 10070.00 |
|
| MR211435L3 | Lenti ORF clone of Ahi1 (Myc-DDK-tagged) - Mouse Abelson helper integration site (cDNA clone MGC:62786 IMAGE:6467369) |
CNY 11970.00 |
|
| MR211435L4 | Lenti ORF clone of Ahi1 (mGFP-tagged) - Mouse Abelson helper integration site (cDNA clone MGC:62786 IMAGE:6467369) |
CNY 11970.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
Mouse monoclonal turboGFP antibody, clone OTI2H8
