Tsc22d1 (BC058660) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	TSC, TSC-22
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211553 representing BC058660 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCACCCGGCAATGTTCCCTCGAAGGGGCAGCGGTGGTGGCAGCGCCTCTGCTCTCAATGCAGCAG GTACGGGCGTCAGTGGTGCTGCTCCATCTTCTGAAGATTTTCCTCCTCCTTCGCTGCTCCAGCCGCCGCC TCCTGCTGCATCTTCCACGCAGGGACCCCAGCCTCCGCCTCCACAAAGCCTGAACCTCCTCTCGCAGGCT CAGCTGCAGGGACAGCCTCTTGCGCCAGGCGGAACTCAGATGAAAAAGAAAAGTGGCTTCCAGATAACTA GCGTTACTCCGGCTCAGATCTCTGCCAGCATCAGCTCCAACAACAGCATCGCGGAGGACACCGAGAGCTA CGACGATCTGGATGAGTCGCACACGGAAGATCTCTCCTCTTCCGAGATCCTCGACGTCTCCCTTTCCAGG GCCACTGACTTAGGTGAGCCTGAACGAAGCTCCTCAGAGGAGACTCTGAATAACTTCCAGGAAGCTGAGA CACCTGGGGCAGTCTCTCCTAACCAGCCCCACCTTCCTCAGCCTCATTTGCCTCACCTTCCACAACAGAA CGTTGTGATCAATGGGAATGCCCATCCCCACCACCTCCATCATCACCATCACCCTCATCATGGGCACCAC CTTCACCATGGGCACCATCATTCATCACATGCTGCTGTGGCCGGTCCATCCATTCCCGGAGGGCCACCCT CGAGCCCAGTGTCCAGGAAACTCTCTACAACTGGAAGTTCTGATGGTGGTGTGCCAGTCGCACCTCCTCC TGCAGTACCATCGAGTGGCTTACCAGCATCAGTGATGACGAACATCCGTACTCCAAGTACTACAGGAAGC CTAGGTATAAATTCGGTTACTGGCACGAGCGCTACGAATAATGTTAACATTGCTGCTGTGGGTAGTTTCA GTCCCAGTGTGACGAACAGCGTGCATGGTAATGCTAATATAAATACAAGCAATATCCCTAATGCTGCTAG CATAAGTGGTGGGCCTGGAGTTACCAGTGTTGTTAATTCGAGTATCTTGAGTGGCATGGGCAATGGTACC GTTTCTTCCTCTCCTGTTGCTAACAGTGTCCTTAATGCAGCTGCAGGTATCACTGTGGGAGTGGTTTCCA GTCAGCAGCAGCAGCAGCAGCAGCAGCAACCAACAGTTAACACATCGAGGTTCAGGGTTGTGAAGTTAGA CTCTACTTCTGAACCCTTTAAAAAAGGTCGATGGACTTGCACAGAATTCTATGAGAAGGAAAACGCTGTG CCAGCTACAGAAGGCGTGGCCGTCAATAAAGTGGTGGAGACGGTGAAGCAAACCCCCACGGAAGCATCGT CCTCGGAGAGGGAGAGCACTAGTGGGAGTTCTGTGAGCAGTAGTGTGAGCACACTGAGTCACTACACGGA GAGTGTGGGAAGTGGAGAGATGATGGGAGCCCCGGCTGTGGTGGCGCCGCAGCAGCCGCCGCTACCACCA GCGCCTCCAGGTCTTCAAGGTGTGGCTCTCCAACAGCTAGAGTTCAGTAGCCCTGCTCCACAGAGTATTG CGGCGGTTAGTATGCCACAGAGTATTTCTCAGTCACAGATGTCACAAGTACAGTTACAGCCTCAAGAGTT GAGCTTTCAGCAGAAGCAGACTCTTCAGCCTGTCCCTCTGCAAGCCACCATGAGTGCCGCAACTGGTATC CAGCCTTCCCCTGTCAGCGTGGTCGGCATCACGGCGGCTGTAGGTCAGCAGCCTTCTGTTTCCAGCCTGG CTCAACCGCAGCTGCCATATTCTCAGACAGCTCCTCCCGTGCAAACTCCTCTTCCAGGGGCACCACCCCA GCAGTTACAATATGGGCAGCAGCAACCAATGGTTCCTGCGCAGATAGCCCCAGGCCATGGCCAGCCAGTG ACTCAAAATCCAACTTCAGAGTATGTGCAGCAGCAGCAGCAGCCAATATTTCAAGCAGCATTGTCCTCTG GGCAGTCCAGTTCCACGGGCACGGGGGCAGGAATATCAGTGATTCCTGTGGCTCAGGCACAGGGGATCCA GCTGCCAGGGCAGCCCACAGCAGTACAAACACAACCTGCAGGGGCGGCTGGGCAGCCCATTGGCCAGGCT CAAACAGCAGTGTCCACTGTACCAACTGGCGGTCAGATTGCAAGTATCGGTCAACAGGCAAACATACCCA CTGCAGTACAGCAGCCCTCTACCCAAGTTACACCTTCAGTTATTCAGCAAGGTGCTCCTCCATCTTCACA AGTAGTCCTACCTGCTCCAACCGGGATCATTCATCAGGGAGTTCAAACCCGTGCTTCAAGCCTTCCACAA CAATTGGTCATTGCACCCCAGAGTACCTTGGTAACTGTGCCTCCCCAGCCACAGGGAGTAGAAACCGTGG CCCAAGGGGTTGTTTCCCAGCAGTTGCCCACAGGCAGTCCTCTGCCCTCTGCTAGCACTATTTCTGTTAC AAATCAGGTTAGTTCAGCCGCTCCTTCTGGAATGCCTTCTGTCCCAACAAACTTAGTTCCACCACAGAAT ATAGCACAACCCCCAGCCACCCAAAATGGCAGTTTGGTTCAAAGTGTTAGTCAATCTCCCTTGATAGCCA CTAACATAAATTTGCCTTTGGCACAACAGATACCACTAAGTTCTACTCAGTTCTCTACACAATCATTAGC TCAGGCCATTGGAAGCCAAATGGAAGATGCCAGGCGCCCAGCGGAGCCCTCCTTAGGCGGCTTACCTCAG ACTATGAGTGGTGACAGTGGGGGAATGTCAGCAGTTTCAGATGGGAGTAGCAGCAGCCTAGCAGCCCCTG CTTCTCTCTTCCCGTTGAAGGTGCTACCGCTGACAACACCCCTGGTGGATGGCGAGGACGAGAGCTCTGG TGCAAGTGTGGTAGCTATCGACAACAAAATAGAGCAAGCTATGGATCTGGTGAAAAGCCATTTGATGTAT GCGGTGAGGGAGGAAGTGGAAGTTCTGAAGGAGCAGATCAAAGAACTAATAGAGAAAAACTCCCAGCTGG AGCAGGAGAACAATCTGCTGAAGACGCTGGCCAGTCCGGAGCAGCTCGCCCAGTTTCAGGCCCAGCTGCA GACTGGCTCCCCTCCGGCCACCACGCAGCCACAGGGGACCACACAGCCCCCTGCACAGCCAGCATCCCAG GGCTCAGGATCAACCGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211553 representing BC058660 Red=Cloning site Green=Tags(s) MAHPAMFPRRGSGGGSASALNAAGTGVSGAAPSSEDFPPPSLLQPPPPAASSTQGPQPPPPQSLNLLSQA QLQGQPLAPGGTQMKKKSGFQITSVTPAQISASISSNNSIAEDTESYDDLDESHTEDLSSSEILDVSLSR ATDLGEPERSSSEETLNNFQEAETPGAVSPNQPHLPQPHLPHLPQQNVVINGNAHPHHLHHHHHPHHGHH LHHGHHHSSHAAVAGPSIPGGPPSSPVSRKLSTTGSSDGGVPVAPPPAVPSSGLPASVMTNIRTPSTTGS LGINSVTGTSATNNVNIAAVGSFSPSVTNSVHGNANINTSNIPNAASISGGPGVTSVVNSSILSGMGNGT VSSSPVANSVLNAAAGITVGVVSSQQQQQQQQQPTVNTSRFRVVKLDSTSEPFKKGRWTCTEFYEKENAV PATEGVAVNKVVETVKQTPTEASSSERESTSGSSVSSSVSTLSHYTESVGSGEMMGAPAVVAPQQPPLPP APPGLQGVALQQLEFSSPAPQSIAAVSMPQSISQSQMSQVQLQPQELSFQQKQTLQPVPLQATMSAATGI QPSPVSVVGITAAVGQQPSVSSLAQPQLPYSQTAPPVQTPLPGAPPQQLQYGQQQPMVPAQIAPGHGQPV TQNPTSEYVQQQQQPIFQAALSSGQSSSTGTGAGISVIPVAQAQGIQLPGQPTAVQTQPAGAAGQPIGQA QTAVSTVPTGGQIASIGQQANIPTAVQQPSTQVTPSVIQQGAPPSSQVVLPAPTGIIHQGVQTRASSLPQ QLVIAPQSTLVTVPPQPQGVETVAQGVVSQQLPTGSPLPSASTISVTNQVSSAAPSGMPSVPTNLVPPQN IAQPPATQNGSLVQSVSQSPLIATNINLPLAQQIPLSSTQFSTQSLAQAIGSQMEDARRPAEPSLGGLPQ TMSGDSGGMSAVSDGSSSSLAAPASLFPLKVLPLTTPLVDGEDESSGASVVAIDNKIEQAMDLVKSHLMY AVREEVEVLKEQIKELIEKNSQLEQENNLLKTLASPEQLAQFQAQLQTGSPPATTQPQGTTQPPAQPASQ GSGSTA TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC058660
ORF Size	3168 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC058660, AAH58660
RefSeq Size	4601 bp
RefSeq ORF	3170 bp
Locus ID	21807
Gene Summary	Transcriptional repressor. Acts on the C-type natriuretic peptide (CNP) promoter.[UniProtKB/Swiss-Prot Function]

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