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Eif2ak3 (NM_010121) Mouse Tagged ORF Clone
CAT＃: MG211654
- TrueORF®
Eif2ak3 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 3 (Eif2ak3)
ORF Plasmid: DDK
"NM_010121" in other vectors (4)
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CNY 10,740.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PE; Pek; Perk |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211654 representing NM_010121
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGCGCCACCCGGCCCGGGCCGCGCGCGCTGCTGCTGCTTCTGTTCCTGCTGCTGGGCTGCGCGG CGGGGATCTCGGCGGTCGCGCCCGCCCGCAGTTTGCTTGCTCCCGCGTCGGAGACAGTGTTTGGCTTAGG GGCAGCGGCCGCCCCGACTTCGGCCGCGCGGGTGCCTGCGGTGGCAACGGCCGAAGTGACCGTGGAGGAC GCCGAGGCATTGCCGGCTGCCGCTGGCGAACCGGAGTCACGCGCGACGGAGCCCGATGACGACGTGGAAC TGCGGCCTCGCGGCAGGTCCTTGGTAATCATCAGCACTTTAGATGGACGAATCGCTGCACTGGATGCCGA GAATGATGGGAAAAAGCAGTGGGATTTGGACGTGGGGTCTGGTTCCTTGGTTTCATCTAGCCTCAGCAAG CCAGAGGTGTTTGGGAACAAGATGATCATCCCCTCCCTGGATGGAGACCTCTTCCAGTGGGACCGGGACC GAGAGAGCATGGAGGCCGTCCCCTTCACGGTGGAGTCCCTGCTCGAATCTTCCTACAAGTTTGGAGATGA TGTTGTTCTGGTTGGAGGGAAATCTCTGACTACATACGGACTCAGTGCTTACAGTGGAAAGCTGAGGTAT ATCTGTTCTGCCTTGGGATGTCGCCGATGGGATAGTGATGAAATGGAAGAAGAGGAAGACATCTTGCTTC TGCAGCGTACGCAGAAGACTGTGCGAGCTGTCGGGCCTCGAAGCGGCAGTGAGAAGTGGAATTTCAGTGT TGGCCACTTTGAACTTCGGTATATTCCAGACATGGAAACTAGAGCCGGATTCATTGAAAGCACCTTTAAA CCGGGTGGAAACAAAGAAGACTCTAAAATTATTTCAGATGTGGAAGAACAAGAAGCCACCATGCTGGACA CAGTGATAAAAGTTTCCGTTGCTGATTGGAAGGTCATGGCGTTTAGTAGGAAGGGAGGCCGCCTGGAATG GGAGTACCAGTTTTGTACTCCCATCGCGTCCGCCTGGCTGGTGAGGGATGGCAAGGTCATCCCCATCAGC CTGTTTGATGATACAAGTTACACAGCCAGCGAAGAAGCCTTGGGAGACGAAGAAGACATTGTAGAGGCTG CTCGGGGAGCCACAGAGAACAGCGTGTACTTAGGGATGTACAGAGGCCAGCTGTACCTGCAGTCGTCCGT CAGGGTCTCAGAAAAGTTCCCTACAAGCCCAAAGGCCTTGGAGTCTGTAAATGGCGAAAATGCAATTATT CCTCTGCCGACGATCAAATGGAAGCCCTTAATCCATTCTCCTTCTAGGACTCCTGTCTTGGTTGGGTCTG ATGAATTTGACAAATGTCTAAGTAATGATAAGTATTCCCACGAAGAATACAGTAATGGTGCACTTTCAAT CCTCCAGTATCCATACGATAACGGTTACTATCTGCCATACTACAAGAGAGAAAGGAATAAGCGGAGCACG CAGATCACAGTCAGGTTCCTGGACAGCCCCCACTACAGCAAGAACATCCGCAAGAAGGACCCTATCCTCC TGCTGCACTGGTGGAAGGAGATATTCGGGACGATCCTGCTTTGCATCGTAGCCACGACCTTCATCGTGCG CAGGCTTTTCCATCCTCAGCCCCACAGGCAGCGGAAGGAGTCTGAAACTCAGTGCCAGACTGAAAGTAAA TACGACTCCGTGAGTGCCGATGTCAGTGACAACAGCTGGAATGACATGAAGTACTCAGGATACGTATCCC GATATCTAACAGATTTTGAGCCAATTCAGTGCATGGGTCGTGGTGGCTTTGGCGTTGTCTTTGAAGCTAA AAACAAAGTAGATGACTGCAATTACGCTATCAAGAGGATCCGGCTCCCCAACAGGGAGTTGGCACGGGAG AAGGTAATGCGGGAAGTTAAAGCCTTGGCTAAGCTGGAACACCCAGGCATTGTGAGGTATTTCAACGCCT GGCTGGAAACCCCACCAGAGAAGTGGCAAGAAGAGATGGATGAGATCTGGCTCAAAGACGAAAGCACAGA CTGGCCGCTCAGCTCCCCTAGCCCGATGGATGCCCCATCTGTTAAGATCCGAAGGATGGATCCTTTCTCT ACAAAAGAGCAGATCGAAGTCATAGCTCCTTCTCCTGAAAGAAGTCGGTCTTTCTCGGTGGGCATTTCCT GTGGCCAGACAAGCTCATCGGAGAGCCAGTTCTCTCCCCTGGAGTTCTCAGGGACAGACTGCGGAGACAA CAGTGACTCAGCGGACGCAGCCTACAACCTCCAGGACAGTTGCCTGACGGACTGCGAGGACGTGGAAGAT GGCACCGTGGACGGCAATGACGAGGGACACTCCTTTGAACTTTGTCCGTCCGAAGCTTCTCCCTATACCC GGTCAAGGGAAGGAACGTCCTCCTCCATAGTGTTTGAGGACTCTGGCTGCGGCAACGCGTCCAGTAAGGA GGAGCCCAGAGGGAACCGGCTGCATGATGGCAACCATTATGTTAATAAGCTAACTGATCTCAAGTGCTCC AGCAGCAGGTCTTCTTCAGAAGCCACCACCTTGTCTACCTCCCCTACCAGGCCAACCACTCTAAGCTTGG ATTTCACCAAGAACACTGTGGGCCAGCTCCAGCCCAGCTCCCCCAAGGTGTATCTGTACATTCAGATGCA GCTGTGCAGGAAGGAGAACCTCAAAGACTGGATGAACCGGCGCTGCAGCTTGGAGGACCGGGAGCACGGC GTGTGCCTGCACATCTTCCTGCAGATCGCAGAGGCAGTGGAGTTCCTGCACAGCAAGGGACTCATGCACA GGGACCTCAAGCCTTCCAACATATTCTTCACAATGGATGATGTGGTCAAGGTTGGGGACTTTGGACTGGT GACTGCTATGGACCAAGATGAAGAAGAGCAGACTGTACTGACTCCAATGCCAGCCTATGCTACGCACACG GGACAAGTAGGGACCAAGCTATACATGAGCCCAGAGCAGATTCATGGAAACAACTACTCCCATAAAGTGG ACATCTTCTCTTTAGGCTTGATTCTGTTTGAACTCCTCTACCCATTCAGCACCCTGATGGAACGAGTCCG GATTTTAACTGATGTCAGAAATCTCAAGTTTCCTCTACTGTTCACTCAGAAATATCCCCAAGAGCATATG ATGGTTCAAGACATGCTCTCTCCATCCCCCACGGAGCGGCCTGAAGCCACAGACATCATTGAAAATGCCA TATTTGAGAACTTGGAGTTTCCCGGGAAAACGGTTCTGAGACAGCGGTCCCGCTCCATGAGTTCATCTGG AACAAAACATTCCAGACAGCCCAGCTGCTCGTACAGCCCACTGCCTGGCAAC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211654 representing NM_010121
Red=Cloning site Green=Tags(s) MERATRPGPRALLLLLFLLLGCAAGISAVAPARSLLAPASETVFGLGAAAAPTSAARVPAVATAEVTVED AEALPAAAGEPESRATEPDDDVELRPRGRSLVIISTLDGRIAALDAENDGKKQWDLDVGSGSLVSSSLSK PEVFGNKMIIPSLDGDLFQWDRDRESMEAVPFTVESLLESSYKFGDDVVLVGGKSLTTYGLSAYSGKLRY ICSALGCRRWDSDEMEEEEDILLLQRTQKTVRAVGPRSGSEKWNFSVGHFELRYIPDMETRAGFIESTFK PGGNKEDSKIISDVEEQEATMLDTVIKVSVADWKVMAFSRKGGRLEWEYQFCTPIASAWLVRDGKVIPIS LFDDTSYTASEEALGDEEDIVEAARGATENSVYLGMYRGQLYLQSSVRVSEKFPTSPKALESVNGENAII PLPTIKWKPLIHSPSRTPVLVGSDEFDKCLSNDKYSHEEYSNGALSILQYPYDNGYYLPYYKRERNKRST QITVRFLDSPHYSKNIRKKDPILLLHWWKEIFGTILLCIVATTFIVRRLFHPQPHRQRKESETQCQTESK YDSVSADVSDNSWNDMKYSGYVSRYLTDFEPIQCMGRGGFGVVFEAKNKVDDCNYAIKRIRLPNRELARE KVMREVKALAKLEHPGIVRYFNAWLETPPEKWQEEMDEIWLKDESTDWPLSSPSPMDAPSVKIRRMDPFS TKEQIEVIAPSPERSRSFSVGISCGQTSSSESQFSPLEFSGTDCGDNSDSADAAYNLQDSCLTDCEDVED GTVDGNDEGHSFELCPSEASPYTRSREGTSSSIVFEDSGCGNASSKEEPRGNRLHDGNHYVNKLTDLKCS SSRSSSEATTLSTSPTRPTTLSLDFTKNTVGQLQPSSPKVYLYIQMQLCRKENLKDWMNRRCSLEDREHG VCLHIFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAMDQDEEEQTVLTPMPAYATHT GQVGTKLYMSPEQIHGNNYSHKVDIFSLGLILFELLYPFSTLMERVRILTDVRNLKFPLLFTQKYPQEHM MVQDMLSPSPTERPEATDIIENAIFENLEFPGKTVLRQRSRSMSSSGTKHSRQPSCSYSPLPGN SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010121 |
| ORF Size | 3342 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010121.2, NP_034251.2 |
| RefSeq Size | 4512 bp |
| RefSeq ORF | 3345 bp |
| Locus ID | 13666 |
| Gene Summary | The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in a similar gene in human are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203473 | Eif2ak3 (untagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 3 (Eif2ak3), (10ug) |
CNY 7,768.00 |
|
| MR211654 | Eif2ak3 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 3 (Eif2ak3) |
CNY 7,760.00 |
|
| MR211654L3 | Lenti ORF clone of Eif2ak3 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 3 (Eif2ak3) |
CNY 11,690.00 |
|
| MR211654L4 | Lenti ORF clone of Eif2ak3 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 3 (Eif2ak3) |
CNY 11,690.00 |
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