Zbed4 (BC052174) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC59429
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211740 representing BC052174 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATAAGCAAGAAACTTGTCCCAAGGGGGACAGTGATTTTGTTTCTGACAAAGTAAATTTCAAAA CGGAGGAAGATGATGATCAAACTCCTTGTCACAGTTTGGAACAAGTGGACTTTAAAAGTGAGTCAGAGGA TATGAGACAGACAGACAGTGGCGATGAACAGGCAGACATCAGGGCAGCGAGCTGTGCCTGTCAGCCCTCT GGGAAGTTCTTAGCTGCCGAGAGTGAGGATGACTATGGCTCACTCTTCTCCCAGTATAGTAGCACACTGT ACAGCGTAGCCATGGAAGCTGTGACCCAGAGCCTTCTTTCCAGTCGACACATAAGCTCCAGGAAGAAGTC TCCAGCCTGGAAGCATTTCTTTATTTCCCCTCGAGATAGCACTAAGGCTATCTGCACATACTGTATGAAA GAGTTCAGTAGGGGCAAGAATGAGAAAGACCTGAGTACAAGTTGCCTCATGAGACATGTGAGGCGGGCAC ACCCCACCAAGCTCATTCAAGAAAATGGAAGTCTATCGGCAGGGTCCTCCTTTTCCCCTCCCTCTCTGTT GCTTCCACCACAGCCTGCAGATGTGGGAGACCTTAGCACTGTGCTCTCGCCTGTCAGACTTGTCCAGAAA ATGGCTCCCAAGATCCCATCCCCTGACCAAATAATGGAAGAGTCTGTGACTGTAGTTTCTTCTGAAGAGT TGTCCTCTGATGTGTCTGTCACTGAAAAGTACAGCAGAGAGGAGGCCTTGGCAGGCTCCTCCCCCAACCT CTCCATGCTCCATTATGACGACGCTTCAGAAACCATGGCAGACAGAAACCTTTCTCTTCCCAAAAGCACA TCTGGGTCCAGGAGGAGGTCAGCCGTCTGGAAGCACTTCTACTTGTCACCCCTTGATAGCTCCAAGGCTG TCTGTGTCCACTGTATGAATGAGTTCAGTAGAGGGAAAAATGGGAAGGACCTGGGTACAAGCTGCCTCAT CAGGCACATGTGGAGAGCACATCGCTCCATTGTACTGCAGGAGAACGGGGGCAACACAGGCATCCCACCA TTGTACCCTATGCCCCCCACCCTGCTGCCTGCCCTGCTGCCCCCAGAAGGGGATCTCAACTCCGCATCGT TGTCTCCAGGCAAACAAGTCAAAGAGTCCCCTTCTGCCTCCTCATCCCCAGAAAGGCTTCCGGAGGACCT GTCATCACACACTAATCCTGGGGATGTATCCCGGGAAGATGTGTCCATGTTGTCATCCTCTGATGACTTG GGGGAGGCCTCTGTAGTGTCTTCTCCAGAGAAGCAGCCAGCGGACACGGTAAATCCAAGGTTTGAATCAG GTACTGTTTTCCAGCAAAATAAGAAGGTGATGAGAAGACTAAAGTCAGAAGTCTGGCATCACTTTTCCCT GGCCCCCATGGACAGTCTCAAAGCAGTGTGCCGGTACTGTAGCTGCGTTATTAGTCGGGGGAAGAAGGGA GATGTTGGCACCAGTTGTTTGATGCGACACCTGTACAGACGCCACCCTGAGGTGGTTGGGAACCAGAAGG ACTTTCTGGGTGCAAGTTTGGCAAACTCTCCATATGCCACTTTGGCTTCTGCAGAAAGCTCCTCCAAGTT AACTGACTTGCCAGCAGTGGTCAGAAAAAACCATCAAGGTGTGTTTCCTACTAATAGCAAGAAGACCTCA AAGCTGTGGAATCACTTTTCTATTTGTTCAGCAGACTCAACAAAGGTGGTGTGCCTGCACTGTGGCCGGA CCATCAGCAGGGGCAAGAAACCCACCAACCTGGGCACCAGCTGTCTCCTGAGACATCTGCAGCGGTTCCA TGGCCATGTACTCAAGAATGATGTCTCAGAGGCCACTCTGTCCCGTTCTCCAGGCATCCGGAGGCCTCTG GGCATAGAGCTCTCAGGGCCTTCCTCTTTCCGTGACTCCACAGAGAAGTTCTATGACTCTCATCCAGTTG CCAAAAAAATCACAAGTCTCATAGCTGAAATGATTGCACTTGACCTTCAGCCATATTCCCTTGTAGACAA CGTTGGCTTTAACAGGCTGCTCGAATACTTGAAACCTCAGTACTCTTTACCCTCCCCTTCCTACTTCTCT AGAACAGCTATCCCAGGTATGTATGACAATGTGAAACAGATAATTATGTCACATCTGAAGGAAGCTGAGA GTGGTGTGGTTCACTTCACCTCTGGAATATGGATGAGTAGCCAGACTCGTGAATACCTGACCCTTACAGC TCACTGGGTCACCTTCGCATCCGCTGTCCGACCACATTGTGAGGACCACCATTGCTCAGCACTTTTAGAT GTCTCACAAATCGACTGTGACTATAGTGGAAATAGCATTCAGAAGCAGCTGGAGTGTTGGTGGGAAGCCT GGGTGACTTCCATTGGCCTTCAGATTGGCATCACTGTCACTGACAATCCAAGCATAGGGAAGATGCTGAG TGAGGGTGAACACTCAAGTGTGCAGTGCTTCAGTCACACAGTGAATCTGATTGTGAGCGAGGCTATCAAG AGCCAGAGGATGGTGCAGAACTTACTGAGTATTGCCCGGAAGCTGTGTGAGCGGGTCCATCGGTCACCCA GAGCAAGAGAGAAGTTAGCAGAACTGCAGAAAGAGTACGAGCTGCCACAGCACCAGCTGATCCAGGATGT ACCGTCTAAGTGGAGCACATCGTTCCACATGCTCGAACGTCTGATTGAGCAGAAGAGGGCAGTTAATGAG GTATCCATTGAGTGTAACTTCAGAGAATTGATCAGCTGTGACCAGTGGGAGGTCATGCAGTCTGTGTGCC ATGTGCTGAGACCATTTGATGCTGCAAGCCGAGAGATGAGTGCCCACATGTCTACGCTGAGCCAGGTCAT CCCCATGATCCACATCCTCAGCAGAAAAGTGGAGATGCTGTTTGGGGAGACCATGGGCATTGACACCATG CTTAAGTCCCTGAAGGAAGCCATGGCAAGCCGCCTGTCTGCCACACTCCATGACCCCAGGTACATCTTTG CCACACTGCTGGACCCTCGCTACAAAGCTTCCCTGTTCACAGAGGAGGAGGCAGAGCAGTACAGGCAAGA CTTAATCAGGGAGCTAGAAATACTGAATTCTACCTCAGAGGACACTGCCACCTCCAATGGCTGTGACTCA GGGTCCCCACTTAAAGACACTGGCACAGAGGAGAGCCTGTGGTCACTCGCACCGATAAAGAGAGATCAGA GAGAGAAGCTACCTGAAGACATGGTGCTTGCGTATTTGGAGGAAGAGGTGCTGGAACACAGCTGTGACCC ACTCACCTACTGGAACCTAAAGCGGTCATCGTGGCCTGGGCTGTCTACCTTAGCAGTTCGATTTCTGGGA TGTCCCCCAAGTACAGTCCCCTCAGAAAAGCTGTTCAGCACACCCATGGATGCTGGCAGCTTTGGCCAGC CCAGGCTCATGATGGAGCATTTTGAAAAGCTCATCTTTTTAAAAGTGAATCTTCCTTTAATATGCTTTCA GTAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211740 representing BC052174 Red=Cloning site Green=Tags(s) MEDKQETCPKGDSDFVSDKVNFKTEEDDDQTPCHSLEQVDFKSESEDMRQTDSGDEQADIRAASCACQPS GKFLAAESEDDYGSLFSQYSSTLYSVAMEAVTQSLLSSRHISSRKKSPAWKHFFISPRDSTKAICTYCMK EFSRGKNEKDLSTSCLMRHVRRAHPTKLIQENGSLSAGSSFSPPSLLLPPQPADVGDLSTVLSPVRLVQK MAPKIPSPDQIMEESVTVVSSEELSSDVSVTEKYSREEALAGSSPNLSMLHYDDASETMADRNLSLPKST SGSRRRSAVWKHFYLSPLDSSKAVCVHCMNEFSRGKNGKDLGTSCLIRHMWRAHRSIVLQENGGNTGIPP LYPMPPTLLPALLPPEGDLNSASLSPGKQVKESPSASSSPERLPEDLSSHTNPGDVSREDVSMLSSSDDL GEASVVSSPEKQPADTVNPRFESGTVFQQNKKVMRRLKSEVWHHFSLAPMDSLKAVCRYCSCVISRGKKG DVGTSCLMRHLYRRHPEVVGNQKDFLGASLANSPYATLASAESSSKLTDLPAVVRKNHQGVFPTNSKKTS KLWNHFSICSADSTKVVCLHCGRTISRGKKPTNLGTSCLLRHLQRFHGHVLKNDVSEATLSRSPGIRRPL GIELSGPSSFRDSTEKFYDSHPVAKKITSLIAEMIALDLQPYSLVDNVGFNRLLEYLKPQYSLPSPSYFS RTAIPGMYDNVKQIIMSHLKEAESGVVHFTSGIWMSSQTREYLTLTAHWVTFASAVRPHCEDHHCSALLD VSQIDCDYSGNSIQKQLECWWEAWVTSIGLQIGITVTDNPSIGKMLSEGEHSSVQCFSHTVNLIVSEAIK SQRMVQNLLSIARKLCERVHRSPRAREKLAELQKEYELPQHQLIQDVPSKWSTSFHMLERLIEQKRAVNE VSIECNFRELISCDQWEVMQSVCHVLRPFDAASREMSAHMSTLSQVIPMIHILSRKVEMLFGETMGIDTM LKSLKEAMASRLSATLHDPRYIFATLLDPRYKASLFTEEEAEQYRQDLIRELEILNSTSEDTATSNGCDS GSPLKDTGTEESLWSLAPIKRDQREKLPEDMVLAYLEEEVLEHSCDPLTYWNLKRSSWPGLSTLAVRFLG CPPSTVPSEKLFSTPMDAGSFGQPRLMMEHFEKLIFLKVNLPLICFQY TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC052174
ORF Size	3504 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC052174, AAH52174
RefSeq Size	5307 bp
RefSeq ORF	3506 bp
Locus ID	223773
Gene Summary	Transcriptional regulator that binds to poly-guanine tracts in gene promoters and activates transcription (By similarity). Able to bind single- and double-stranded DNA and RNA (PubMed:22693546).[UniProtKB/Swiss-Prot Function]

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