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Plcg1 (NM_021280) Mouse Tagged ORF Clone
CAT＃: MG211903
- TrueORF®
Plcg1 (tGFP-tagged) - Mouse phospholipase C, gamma 1 (Plcg1)
ORF Plasmid: DDK
"NM_021280" in other vectors (4)
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CNY 10,550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI894140; Cded; Plc-1; Plc-gamma1; Plcg-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211903 representing NM_021280
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCGTCGCGACCCCCTGCGCCAACGGCTGCGGGCCTGGCGCACCCTCCGAAGCCGAGGTGCTGC ACCTCTGCCGCAGCCTCGAGGTGGGCACCGTCATGACTTTGTTCTACTCCAAGAAGTCGCAGCGGCCAGA ACGGAAGACCTTCCAGGTCAAGTTGGAGACGCGCCAGATCACATGGAGCCGCGGCGCGGACAAAATCGAG GGGTCCATTGATATCCGAGAAATCAAGGAGATCCGTCCAGGGAAGACTTCTCGGGACTTTGACCGCTACC AAGAAGACCCTGCCTTCCGGCCAGATCAATCACACTGCTTTGTCATCCTCTATGGAATGGAATTCCGCTT GAAGACCCTGAGCCTGCAAGCCACATCTGAGGATGAAGTGAACATGTGGATCAAGGGCTTAACTTGGCTA ATGGAGGATACACTGCAGGCAGCCACACCCCTGCAAATTGAGAGGTGGCTCCGGAAGCAGTTCTACTCAG TGGATCGTAACCGAGAGGATCGTATATCAGCCAAGGACTTGAAGAACATGCTGTCTCAGGTCAACTACCG AGTCCCCAATATGCGCTTCCTCCGAGAGCGGCTGACGGACCTTGAACAGCGCAGCGGGGACATCACCTAC GGGCAGTTTGCTCAGCTGTACCGCAGCCTCATGTACAGCGCCCAGAAGACGATGGACCTTCCCTTCTTGG AAACCAACGCTTTGAGGACTGGAGAGCGTCCGGAGCATTGCCAGGTGTCCCTTTCTGAGTTCCAGCAGTT CCTCCTTGAATACCAGGGGGAGCTGTGGGCTGTCGACCGGCTCCAGGTGCAGGAATTCATGCTCAGCTTC CTTCGAGACCCCTTGCGAGAGATTGAGGAGCCATACTTCTTCTTGGATGAGCTTGTCACCTTCCTGTTCT CCAAAGAGAACAGCGTGTGGAACTCACAGCTGGATGCTGTGTGCCCGGACACCATGAACAACCCACTCTC TCACTATTGGATCTCCTCCTCACATAATACGTACCTGACTGGGGACCAGTTCTCCAGTGAGTCCTCCCTG GAAGCCTATGCTCGCTGCCTGAGGATGGGCTGTCGCTGCATTGAGTTGGACTGCTGGGATGGGCCAGATG GGATGCCAGTCATTTACCATGGGCACACTCTCACCACCAAGATCAAGTTCTCTGACGTCCTGCACACCAT CAAGGAGCATGCTTTCGTAGCCTCAGAGTACCCAGTCATCCTGTCCATCGAGGACCACTGCAGCATTGCC CAGCAGAGAAACATGGCTCAGCACTTCAGAAAGGTGCTTGGCGACACACTCCTTACCAAGCCTGTGGACA TTGCTGCTGATGGCCTTCCTTCTCCCAACCAGCTCAGGAGGAAGATCCTTATTAAGCACAAGAAGCTGGC TGAGGGCAGTGCCTATGAGGAGGTGCCTACCTCTGTGATGTACTCCGAGAATGACATCAGTAACTCCATC AAGAATGGTATCCTCTACTTGGAGGACCCCGTGAATCATGAGTGGTATCCCCACTACTTCGTTCTGACTA GCAGCAAGATCTACTACTCTGAGGAGACCAGCAGTGACCAGGGCAATGAGGATGAAGAGGAGCCGAAGGA GGCCAGCAGCAGCACAGAGCTGCACTCGAGTGAGAAGTGGTTCCACGGGAAGCTCGGGGCTGGACGTGAC GGGCGGCACATTGCTGAGCGCCTGCTCACTGAGTACTGCATAGAGACTGGGGCTCCTGATGGCTCCTTCC TAGTGCGAGAAAGTGAGACCTTCGTGGGTGACTACACGCTGTCTTTCTGGCGGAATGGGAAAGTCCAGCA CTGCCGCATCCACTCCCGGCAGGATGCTGGGACTCCTAAGTTCTTCTTGACAGATAACCTTGTCTTTGAC TCTCTCTATGACCTCATCACACATTATCAGCAAGTACCCCTGCGCTGCAATGAGTTTGAGATGCGCCTTT CAGAGCCTGTTCCACAGACGAATGCCCATGAGAGCAAAGAGTGGTACCACGCAAGCCTGACTAGAGCTCA GGCTGAACATATGCTGATGCGAGTGCCCCGGGATGGGGCCTTCCTGGTGCGGAAACGCAATGAGCCTAAC TCATATGCCATCTCTTTCCGGGCTGAGGGAAAGATCAAGCACTGCCGAGTACAGCAGGAAGGCCAGACAG TGATGCTGGGGAACTCTGAGTTTGACAGCCTGGTTGACCTCATCAGCTACTATGAGAAGCACCCCCTGTA CCGCAAAATGAAGCTACGCTACCCCATCAACGAGGAGGCACTGGAGAAGATCGGGACAGCTGAACCCGAT TATGGGGCACTATACGAGGGCCGCAACCCTGGTTTCTATGTGGAGGCAAACCCTATGCCAACTTTCAAGT GTGCAGTAAAAGCCCTCTTCGACTACAAGGCCCAGAGAGAGGATGAGCTGACCTTCACCAAGAGTGCCAT CATCCAGAATGTGGAAAAGCAAGATGGTGGCTGGTGGCGAGGGGACTATGGTGGGAAGAAGCAGCTGTGG TTCCCCTCAAACTATGTGGAAGAGATGATCAATCCAGCAGTCCTAGAGCCTGAGAGGGAGCACCTGGATG AGAACAGCCCACTGGGGGACTTGCTGCGAGGGGTCTTAGATGTGCCAGCTTGTCAGATCGCCATCCGTCC TGAGGGCAAAAACAACCGGCTCTTCGTCTTCTCCATCAGCATGCCATCAGTGGCTCAGTGGTCCCTGGAT GTTGCAGCTGACTCACAGGAGGAGTTACAGGACTGGGTGAAAAAGATCCGTGAAGTTGCCCAGACTGCAG ATGCCAGGCTCACTGAGGGAAAGATGATGGAGAGGAGGAAGAAGATCGCCTTGGAGCTCTCCGAGCTTGT GGTCTACTGCCGGCCCGTTCCCTTTGATGAAGAGAAGATTGGCACAGAACGTGCTTGTTACCGGGACATG TCCTCCTTTCCGGAAACCAAGGCTGAGAAGTATGTGAACAAGGCCAAAGGCAAGAAGTTCCTCCAGTACA ACCGGCTGCAGCTCTCGCGCATCTACCCTAAGGGCCAGAGGCTAGACTCCTCCAATTATGACCCTCTGCC CATGTGGATCTGCGGTAGCCAGCTTGTAGCACTCAATTTCCAGACCCCAGACAAGCCTATGCAGATGAAC CAGGCCCTCTTCATGGCTGGTGGGCATTGTGGCTATGTGCTGCAGCCAAGCACCATGAGAGACGAAGCCT TTGACCCCTTTGATAAGAGCAGTCTCCGAGGTCTGGAACCCTGTGTCATTTGCATTGAGGTGCTGGGGGC CAGGCATCTGCCGAAGAATGGCCGGGGTATTGTGTGTCCTTTTGTGGAGATTGAGGTGGCTGGGGCTGAG TACGACAGCACCAAGCAAAAGACGGAGTTTGTAGTGGACAACGGACTGAACCCTGTGTGGCCTGCTAAGC CCTTCCACTTCCAGATCAGTAACCCAGAGTTTGCCTTTCTGCGCTTTGTGGTGTATGAGGAAGACATGTT TAGTGACCAGAACTTCTTGGCTCAGGCTACTTTCCCAGTAAAAGGCCTGAAGACAGGATATAGAGCAGTG CCTTTGAAGAACAACTACAGTGAAGACCTGGAGTTGGCCTCCCTGCTCATCAAGATTGACATTTTCCCTG CTAAGGAGAACGGTGACCTCAGTCCTTTCAGTGGCATATCCCTAAGGGAACGGGCCTCAGATGCCTCCAG CCAGCTGTTCCATGTCCGGGCCCGGGAAGGGTCCTTTGAAGCCAGATACCAGCAGCCATTTGAAGATTTC CGCATCTCGCAGGAGCATCTAGCAGACCATTTTGACAGTCGGGAACGAAGTACTTCAGATGGTCCTTCCT CAGCCACTAACCTTATTGAGGATCCCTTACATGACAAGCTGTGGAAGTGCTCTCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211903 representing NM_021280
Red=Cloning site Green=Tags(s) MAGVATPCANGCGPGAPSEAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADKIE GSIDIREIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWL MEDTLQAATPLQIERWLRKQFYSVDRNREDRISAKDLKNMLSQVNYRVPNMRFLRERLTDLEQRSGDITY GQFAQLYRSLMYSAQKTMDLPFLETNALRTGERPEHCQVSLSEFQQFLLEYQGELWAVDRLQVQEFMLSF LRDPLREIEEPYFFLDELVTFLFSKENSVWNSQLDAVCPDTMNNPLSHYWISSSHNTYLTGDQFSSESSL EAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIA QQRNMAQHFRKVLGDTLLTKPVDIAADGLPSPNQLRRKILIKHKKLAEGSAYEEVPTSVMYSENDISNSI KNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEASSSTELHSSEKWFHGKLGAGRD GRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLTDNLVFD SLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPN SYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPD YGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFTKSAIIQNVEKQDGGWWRGDYGGKKQLW FPSNYVEEMINPAVLEPEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMPSVAQWSLD VAADSQEELQDWVKKIREVAQTADARLTEGKMMERRKKIALELSELVVYCRPVPFDEEKIGTERACYRDM SSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQMN QALFMAGGHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCVICIEVLGARHLPKNGRGIVCPFVEIEVAGAE YDSTKQKTEFVVDNGLNPVWPAKPFHFQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAV PLKNNYSEDLELASLLIKIDIFPAKENGDLSPFSGISLRERASDASSQLFHVRAREGSFEARYQQPFEDF RISQEHLADHFDSRERSTSDGPSSATNLIEDPLHDKLWKCSL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021280 |
| ORF Size | 3906 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021280.3, NP_067255.2 |
| RefSeq Size | 4435 bp |
| RefSeq ORF | 3909 bp |
| Locus ID | 18803 |
| UniProt ID | Q62077 |
| Gene Summary | Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224181 | Plcg1 (untagged) - Mouse phospholipase C, gamma 1 (Plcg1), (10ug) |
CNY 16,720.00 |
|
| MR211903 | Plcg1 (Myc-DDK-tagged) - Mouse phospholipase C, gamma 1 (Plcg1) |
CNY 9,064.00 |
|
| MR211903L3 | Lenti ORF clone of Plcg1 (Myc-DDK-tagged) - Mouse phospholipase C, gamma 1 (Plcg1) |
CNY 11,500.00 |
|
| MR211903L4 | Lenti ORF clone of Plcg1 (mGFP-tagged) - Mouse phospholipase C, gamma 1 (Plcg1) |
CNY 11,500.00 |
