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Sik3 (NM_027498) Mouse Tagged ORF Clone
CAT＃: MG211912
- TrueORF®
Sik3 (tGFP-tagged) - Mouse cDNA sequence BC033915 (BC033915)
ORF Plasmid: DDK
"NM_027498" in other vectors (4)
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CNY 11,880.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 5730525O22Rik; 9030204A07Rik; AI447252; AI846254; AI849445; mKIAA0999; Qsk; SIK-3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211912 representing NM_027498
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCGGCGGCGAGCGGAGCTGGCGGGGTTGCCGTGGCCGGAGCAGGGGGAGCCGGGCCGGCCG GCCGCCTGCTGCCTCCGCCCGCCGCGGGGCCCCCGGCAGCCCCCGCCGCAGTGCCCCCGGCCGCCGTCCC GGCGCGTCCCACAGCCCCGGCCTCCCGCGGGTCGATGGCCGCTCGCATCGGCTACTACGAGATCGACCGC ACCATCGGCAAGGGCAACTTCGCTGTGGTCAAGCGGGCCACGCACCTCGTCACCAAGGCCAAGGTTGCTA TTAAAATCATAGATAAGAGCCAGCTGGATGAAGAAAACTTGAAGAAGATTTTCCGGGAGGTTCAGATAAT GAAGATGCTTTGCCATCCACACATCATCAGACTCTACCAGGTCATGGAGACAGAGCGCATGATTTACCTG GTGACAGAATACGCTAGCGGAGGGGAGATATTTGACCACTTGGTAGCCCATGGAAGAATGGCAGAGAAGG AAGCTCGACGGAAGTTCAAACAGATCGTCACAGCGGTGTATTTTTGTCACTGTCGGAATATCGTTCATCG TGATTTAAAAGCCGAAAACTTACTTCTGGACGCCAATCTGAATATCAAAATAGCAGACTTTGGCTTCAGC AACCTCTTCACTCCAGGGCAGCTGCTGAAGACGTGGTGTGGCAGCCCTCCCTATGCCGCCCCAGAGCTCT TCGAAGGGAAGGAATATGATGGGCCCAAAGTGGACATATGGAGCCTTGGAGTTGTCCTCTATGTGCTGGT GTGTGGCGCCCTGCCGTTTGATGGGAGCACACTGCAGAATCTGCGGGCCCGCGTGCTGAGTGGCAAGTTC CGCATCCCGTTCTTTATGTCCACAGAGTGTGAGCACTTGATCCGCCACATGCTGGTGTTAGATCCAAATA AGCGCCTCTCAATGGAACAGATCTGCAGGCACAAGTGGATGAAGCTCGGAGATGCAGACCCCAACTTTGA CAGGTTAATAGCGGAGTGCCAGCAACTGAAGGAGGAAAGGCAGTCGGACCCCCTCAACGATGATGTCCTC TTGGCTATGGAAGACATGGGGCTGGACAAGGAGCGCACACTTCAGTCATTAAGGTCAGATGCCTATGACC ACTATAGTGCAATCTACAGCCTGCTGTGCGATCGACACAAGAAACATAAAACTCTGCGTCCCGGAGCACT TCCCAGCATGCCCCAAGCCATGACTTTCCAGGCACCAGTCAATCTCCAGGCGGAGCAGACAGGCACTGCT ATGAACCTTAGCGTCCCTCAAGTTCAGCTGATCAACCCAGAGAACCAAATTATAGAGCCTGACGGGGCTG TGAACTTGGACAGTGATGAGGGTGAAGAGCCTTCTCCAGAAGCCTTGGTTCGCTATTTGTCAATGAGGAG GCACACGGTGGGAGTGGCTGACCCACGCACGGAAGTTATGGAAGATCTGCAGAAGCTGCTGCCTGGCTTT CCTGGAGTAAACCCACAGGGTCCATTCCTACAAGTGGCCCCTAACATGAACTTCACGCACAACCTGTTGC CCATGCAGAGTCTGCAGCCAACTGGGCAGCTTGAGTACAAGGAACAGTCTCTGTTACAGCCGCCTACACT ACAGCTACTGAACGGAATGGGGCCCCTTGGCCGGAGAGCCTCAGATGGAGGCGCCAACATCCAACTGCAT GCCCAGCAGCTGCTCAAGCGCCCACGGGGACCGTCCCCACTTGTCACCATGACACCAGCAGTGCCAGCAG TTACCCCTGTGGATGAGGAGAGCTCGGATGGGGAGCCAGATCAGGAAGCTGTGCAGAGGTACTTGGCAAA TAGGTCCAAAAGGCACACACTGGCCATGACCAGCCCGACAGCTGAGATCCCACCGGACCTGCAACGGCAG CTAGGACAACAGTCTTTCCGTTCCCGGGTCTGGCCTCCTCACCTGGTACCTGACCAGCATCGCTCCACCT ACAAGGACTCCAACACCCTGCATCTCCCTACGGAGCGTTTCTCCCCCGTGCGCCGGTTCTCAGACGGGGC TGCAAGCATCCAGGCCTTCAAAGCTCACCTGGAAAAAATGGGCAACAGCAGCAGCATCAAGCAGTTGCAG CAGGAGTGTGAGCAGCTGCAGAAGATGTACGGGGGGCAGGTGGATGAGAGGACCCTGGAGAAGACCCAGC AGCAGCATATGTTGTACCAGCAGGAGCAGCACCATCAGATCCTCCAGCAGCAAATCCAAGATTCTATTTG TCCTCCTCAGCCCTCCCCACCTCTTCAGGTTGCCTGTGAAAACCAACCAGCCCTCCTCACCCACCAGCTC CAGAGGTTAAGGATTCAACCTTCAAGCCCACCCCCCAACCATCCCAGCAACCATCTCTTCAGACAGCCAA GTAACAGTCCTCCCCCGGTCAGCAGTGCCATGATCACGTCTCACGGTGCTACGTCTCCCTCCCAGTTTCA AGGCTTACCCTCCCATGGTGCAATCTTCCAGCAGCAACCCGAGAACTGTTCCCCGCCTCCCAGTGTAGCA CTAACCTGCCTGGGCCTACAGCAGGCTAGCCAGTCACAGCCAGTGACTATCCAGCTACAGGAGCCAGTTG ACATGCTCAGCAACATGGCCGGAACCGCGGCAGGCTCTGCAGGGCGGAGCATCCCCATCAGCCCCAGCGC CAGTCAGATTCAGATACAGCACCGAGCCAGCCTAATGGCTCCCTTCAGCTATGGGCACCGGCCCTTGTCC AAGCAGCTGAGCGCTGACAGTGCAGAGGCCCACAGCTTGAACATGAATCGGTTCTCCCCTGCCAACTACG ACCAGGCGCATTTACACCCCCATCTGTTTTCGGACCAGTCCCGAGGTTCCCCCAGCAGCTACAGCCCTTC AACAGGAGTGGGGTTTCCTCCAACTCAAGCCCTGAAAGTTCCTCCGCTTGACCAGTTCCCCACTTTCCCT CCCAGTGCCCAGCAGCAGCCACCCCACTATACCACGTCAGCACTACAGCAGGCCCTGCTATCTCCTACAC CGCCAGACTATCCCCGACACCAGCAGGTTCCCCATATCCTTCAAGGACTGCTCTCTCCCCGGCATTCACT CACCGGCCACTCGGACATTCGGCTGCCTCCGGCAGAGTTTGCACAGCTCATCAAAAGGCAGCAACAGCAT CGACAGCAGCAGCAGCAGCAGCAGCAGCAACAAGAATACCATGAATTGTTCAGGCACATGAACCAAGGGG ATGCTGTTAGCCTAGCTCCCAGCCTCGGGGGACAGAATATGACAGAGCAACAGGCTTTATCTTATCAAAA TGCTGACTCGTACCACCGCCACCACACCAGCCCCCAGCATATCTTACAGATCAGGGCGCAAGATTGTATC TCACAGGGTCCCTCGCCCACCCCCACCCATGGCTATGCCCATCAGCCACCACTAATGCATTCGGAGAGTA TGGAGGAAGACTGCTTGTGTGAGGGGCTCAAGGAGGGCTTCCCAGACAAGAGCTCAAGCACACTGACCAA AGGTTGCCACAACAGCCCTCTGCTCTTGTGTACCAGTGGGCCTGGGGACCCTGAGCCTTTGCTGGGAACT GTGAGTCAGGCCCGGGAGCTGGGGATCCATCCCTACGGACACCAGCCAACTGCCACCACGTTCAGTAGAA ATAAGGTGCCCAGCCGAGAGTCTGTCCTAGGGAACTGCCTGGAAAGAAGTTCTCCTGGACAAGCAATGGA GCTGCCGGATCACAACGGCCTTGGGTACCCAGTACGGCCCTTAGTCAGTGAGCACCTCAGGTCCCGGACG CTCCAGAGACACCACACGATCCAGAACAGCGACGATGCCTATGTACAGCTGGATACCTTGCCAGGAATGA GCCTGGTGGCAGGCAAGGCGCTGAGCTCTGCCCGGATGTCAGATGCAGTTCTTAGTCAGTCTTCACTCAT GGGCAGCCAACAGTTTCAGGATGAGGAAGATGAAGAATGTGGGGTGAGCCTGGGCCACGAGCATCCAGGC CTGGGTGATGGCAGCCAGCATCTCAACTCCTCTCGCTATCCAGCTACGTGTGTTACAGACATCATGCTCA GCCACAAGCACCCAGAGGTCTCCTTCAGCATGGAGCAAGCCGGCGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211912 representing NM_027498
Red=Cloning site Green=Tags(s) MAAAAASGAGGVAVAGAGGAGPAGRLLPPPAAGPPAAPAAVPPAAVPARPTAPASRGSMAARIGYYEIDR TIGKGNFAVVKRATHLVTKAKVAIKIIDKSQLDEENLKKIFREVQIMKMLCHPHIIRLYQVMETERMIYL VTEYASGGEIFDHLVAHGRMAEKEARRKFKQIVTAVYFCHCRNIVHRDLKAENLLLDANLNIKIADFGFS NLFTPGQLLKTWCGSPPYAAPELFEGKEYDGPKVDIWSLGVVLYVLVCGALPFDGSTLQNLRARVLSGKF RIPFFMSTECEHLIRHMLVLDPNKRLSMEQICRHKWMKLGDADPNFDRLIAECQQLKEERQSDPLNDDVL LAMEDMGLDKERTLQSLRSDAYDHYSAIYSLLCDRHKKHKTLRPGALPSMPQAMTFQAPVNLQAEQTGTA MNLSVPQVQLINPENQIIEPDGAVNLDSDEGEEPSPEALVRYLSMRRHTVGVADPRTEVMEDLQKLLPGF PGVNPQGPFLQVAPNMNFTHNLLPMQSLQPTGQLEYKEQSLLQPPTLQLLNGMGPLGRRASDGGANIQLH AQQLLKRPRGPSPLVTMTPAVPAVTPVDEESSDGEPDQEAVQRYLANRSKRHTLAMTSPTAEIPPDLQRQ LGQQSFRSRVWPPHLVPDQHRSTYKDSNTLHLPTERFSPVRRFSDGAASIQAFKAHLEKMGNSSSIKQLQ QECEQLQKMYGGQVDERTLEKTQQQHMLYQQEQHHQILQQQIQDSICPPQPSPPLQVACENQPALLTHQL QRLRIQPSSPPPNHPSNHLFRQPSNSPPPVSSAMITSHGATSPSQFQGLPSHGAIFQQQPENCSPPPSVA LTCLGLQQASQSQPVTIQLQEPVDMLSNMAGTAAGSAGRSIPISPSASQIQIQHRASLMAPFSYGHRPLS KQLSADSAEAHSLNMNRFSPANYDQAHLHPHLFSDQSRGSPSSYSPSTGVGFPPTQALKVPPLDQFPTFP PSAQQQPPHYTTSALQQALLSPTPPDYPRHQQVPHILQGLLSPRHSLTGHSDIRLPPAEFAQLIKRQQQH RQQQQQQQQQQEYHELFRHMNQGDAVSLAPSLGGQNMTEQQALSYQNADSYHRHHTSPQHILQIRAQDCI SQGPSPTPTHGYAHQPPLMHSESMEEDCLCEGLKEGFPDKSSSTLTKGCHNSPLLLCTSGPGDPEPLLGT VSQARELGIHPYGHQPTATTFSRNKVPSRESVLGNCLERSSPGQAMELPDHNGLGYPVRPLVSEHLRSRT LQRHHTIQNSDDAYVQLDTLPGMSLVAGKALSSARMSDAVLSQSSLMGSQQFQDEEDEECGVSLGHEHPG LGDGSQHLNSSRYPATCVTDIMLSHKHPEVSFSMEQAGV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027498 |
| ORF Size | 4107 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027498.3, NP_081774.3 |
| RefSeq Size | 6296 bp |
| RefSeq ORF | 4110 bp |
| Locus ID | 70661 |
| UniProt ID | Q6P4S6 |
| Gene Summary | Positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor (By similarity). Required for chondrocyte hypertrophy during skeletogenesis (PubMed:22318228). Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3 (By similarity). Prevents HDAC4 translocation to the nucleus (PubMed:22318228).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224338 | Sik3 (untagged) - Mouse SIK family kinase 3 (Sik3), (10ug) |
CNY 10,312.00 |
|
| MR211912 | Sik3 (Myc-DDK-tagged) - Mouse SIK family kinase 3 (Sik3) |
CNY 9,368.00 |
|
| MR211912L3 | Lenti ORF clone of Sik3 (Myc-DDK-tagged) - Mouse SIK family kinase 3 (Sik3) |
CNY 11,768.00 |
|
| MR211912L4 | Lenti ORF clone of Sik3 (mGFP-tagged) - Mouse SIK family kinase 3 (Sik3) |
CNY 11,768.00 |
