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Wrn (NM_011721) Mouse Tagged ORF Clone
CAT＃: MG211966
- TrueORF®
Wrn (tGFP-tagged) - Mouse Werner syndrome homolog (human) (Wrn)
"NM_011721" in other vectors (7)
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CNY 15,390.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI846146 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211966 representing NM_011721
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAACCACTTCACTACAGCGGAAATTTCCAGAATGGATGTCTATGCAGAGTCAAAGATGTGCTACAG AAGAAAAGGCCTGCGTTCAGAAGAGTGTTCTTGAAGATAACCTCCCATTCTTAGAATTCCCTGGATCCAT TGTTTACAGTTATGAAGCTAGTGATTGCTCCTTCCTGTCTGAAGACATTAGCATGCGTCTGTCTGATGGC GATGTGGTGGGATTTGACATGGAATGGCCGCCCATATACAAGCCAGGGAAAAGAAGCAGAGTCGCAGTGA TCCAGTTGTGTGTGTCTGAGAGCAAATGTTACTTGTTTCACATTTCTTCCATGTCAGTTTTCCCCCAGGG ATTAAAAATGTTACTAGAAAACAAATCAATTAAGAAGGCAGGGGTTGGGATTGAAGGGGACCAGTGGAAA CTTCTGCGTGATTTTGACGTCAAGTTGGAGAGTTTTGTGGAGCTGACGGATGTTGCCAATGAAAAGTTGA AGTGCGCAGAGACCTGGAGCCTCAATGGTCTGGTTAAACACGTCTTAGGGAAACAACTTTTGAAAGACAA GTCCATCCGCTGCAGCAATTGGAGTAATTTCCCCCTCACTGAGGACCAGAAACTGTATGCAGCCACTGAT GCTTATGCTGGTCTTATCATCTATCAAAAATTAGGAAATTTGGGTGATACTGCGCAAGTGTTTGCTCTAA ATAAAGCAGAGGAAAACCTACCTCTGGAGATGAAGAAACAGTTGAATTCAATCTCCGAAGAAATGAGGGA CCTAGCCAATCGTTTTCCTGTCACTTGCAGAAATTTGGAAACTCTCCAGAGGGTTCCTGTAATATTGAAG AGTATTTCAGAAAATCTCTGTTCATTGAGAAAAGTGATCTGTGGTCCTACAAACACTGAGACTAGACTGA AGCCGGGCAGTAGTTTTAATTTACTGTCATCAGAGGATTCAGCTGCTGCTGGAGAAAAAGAGAAACAGAT TGGAAAACATAGTACTTTTGCTAAAATTAAAGAAGAACCATGGGACCCAGAACTTGACAGTTTAGTGAAG CAAGAGGAGGTTGATGTATTTAGAAATCAAGTGAAGCAAGAAAAAGGTGAATCTGAAAATGAAATAGAAG ACAATCTGTTGAGAGAAGATATGGAAAGAACTTGTGTGATTCCTAGTATTTCAGAAAATGAACTCCAAGA TTTGGAACAGCAAGCTAAAGAAGAAAAATATAATGATGTTTCTCACCAACTTTCTGAGCATTTATCTCCC AATGATGATGAGAATGACTCCTCCTATATAATTGAAAGTGATGAAGATTTGGAAATGGAGATGCTGAAGT CTTTAGAAAACCTAAATAGTGACGTGGTGGAACCCACTCACTCTACATGGTTGGAAATGGGAACCAATGG GCGTCTTCCTCCTGAGGAGGAAGATGGACACGGAAATGAAGCCATCAAAGAGGAGCAGGAAGAAGAGGAC CATTTATTGCCGGAACCCAACGCAAAGCAAATTAATTGCCTCAAGACCTATTTCGGACACAGCAGTTTTA AACCGGTTCAGTGGAAAGTCATCCATTCTGTATTAGAAGAGAGAAGAGATAATGTTGTTGTCATGGCAAC TGGATATGGGAAGAGTCTGTGCTTCCAGTATCCGCCTGTTTATACAGGCAAGATTGGCATTGTCATTTCA CCTCTCATTTCCTTAATGGAAGACCAAGTCCTCCAGCTTGAGCTGTCCAATGTTCCAGCCTGTTTACTTG GATCTGCACAGTCAAAAAATATTCTAGGAGATGTTAAATTAGGCAAATATAGGGTCATCTACATAACTCC AGAGTTCTGTTCTGGTAACTTGGATCTACTCCAGCAACTTGACTCTAGTATTGGCATCACTCTCATTGCT GTGGATGAGGCTCACTGCATTTCAGAGTGGGGCCATGATTTCAGAAGTTCATTCAGGATGCTGGGCTCTC TTAAAACAGCGCTCCCATTGGTTCCAGTCATTGCACTCTCCGCTACTGCAAGCTCTTCCATCCGGGAAGA CATTATAAGCTGCTTAAACCTGAAAGACCCTCAGATCACCTGCACTGGATTTGATCGGCCAAATCTGTAC TTAGAAGTTGGACGGAAAACAGGGAACATCCTTCAGGATCTAAAGCCGTTTCTCGTCCGAAAGGCAAGTT CTGCCTGGGAATTTGAAGGTCCAACCATCATCTATTGTCCTTCGAGAAAAATGACAGAACAAGTTACTGC TGAACTTGGGAAACTGAACTTAGCCTGCAGAACATACCACGCTGGCATGAAAATTAGCGAAAGGAAGGAC GTTCATCATAGGTTCCTGAGAGATGAAATTCAGTGTGTTGTAGCTACTGTAGCTTTTGGAATGGGCATTA ATAAAGCTGACATTCGCAAAGTTATTCATTATGGTGCGCCTAAGGAAATGGAATCCTATTACCAGGAAAT TGGTAGAGCTGGCCGGGATGGACTTCAGAGTTCCTGTCACTTGCTCTGGGCTCCAGCAGACTTTAACACA TCCAGGAATCTCCTTATTGAGATTCACGATGAAAAGTTCCGGTTATATAAATTAAAGATGATGGTAAAGA TGGAAAAATACCTTCACTCCAGTCAGTGTAGGCGACGAATCATCTTGTCCCATTTTGAGGACAAATGTCT GCAGAAGGCCTCCTTGGACATTATGGGAACTGAAAAATGCTGTGATAATTGCAGGCCCAGGCTGAATCAT TGCCTTACTGCTAACAACTCAGAGGACGCATCCCAAGACTTTGGGCCACAAGCATTCCAGCTACTGTCTG CTGTGGACATCCTGCAGGAGAAATTTGGAATTGGGATTCCGATCTTATTTCTCCGAGGATCTAATTCTCA GCGTCTTCCTGATAAATATCGGGGTCACAGGCTCTTTGGTGCTGGAAAGGAGCAAGCAGAAAGTTGGTGG AAGACCCTTTCTCACCATCTCATAGCTGAAGGATTCTTGGTAGAAGTTCCCAAGGAAAACAAATATATAA AGACATGTTCCCTCACAAAAAAGGGTAGAAAGTGGCTTGGAGAAGCCAGTTCGCAGTCTCCTCCGAGCCT TCTCCTTCAAGCTAATGAAGAGATGTTTCCAAGGAAAGTTCTGCTACCAAGTTCTAATCCTGTATCTCCA GAAACGACGCAACATTCCTCTAATCAAAACCCAGCTGGATTAACTACCAAGCAGTCTAATTTGGAGAGAA CGCATTCTTACAAAGTGCCTGAGAAAGTTTCTTCTGGGACTAACATTCCTAAAAAAAGTGCCGTGATGCC GTCACCAGGAACATCTTCCAGCCCCTTAGAACCTGCCATCTCAGCCCAAGAGCTGGACGCTCGGACTGGG CTATATGCCAGGCTGGTGGAAGCAAGGCAGAAACACGCTAATAAGATGGATGTACCTCCAGCTATTTTAG CAACAAACAAGGTTCTGCTGGACATGGCTAAAATGAGACCGACTACTGTTGAAAACATGAAACAGATCGA CGGTGTCTCTGAAGGCAAAGCTGCTCTGTTGGCCCCTCTGTTGGAAGTCATCAAACATTTCTGTCAAGTA ACTAGTGTTCAGACAGACCTCCTTTCCAGTGCCAAACCTCACAAGGAACAGGAGAAAAGTCAGGAGATGG AAAAGAAAGACTGCTCACTCCCCCAGTCTGTGGCCGTCACATACACTCTATTCCAGGAAAAGAAAATGCC CTTACACAGCATAGCTGAGAACAGGCTCCTGCCTCTCACAGCAGCCGGCATGCACTTAGCCCAGGCGGTG AAAGCCGGCTACCCCCTGGATATGGAGCGAGCTGGCCTGACCCCAGAGACTTGGAAGATTATTATGGATG TCATCCGAAACCCTCCCATCAACTCAGATATGTATAAAGTTAAACTCATCAGAATGTTAGTTCCTGAAAA CTTAGACACGTACCTCATCCACATGGCGATTGAGATTCTTCAGAGTGGTTCCGACAGCAGAACCCAGCCT CCTTGTGATTCCAGCAGGAAGAGGCGTTTCCCCAGCTCTGCAGAGAGTTGTGAGAGCTGTAAGGAGAGCA AAGAGGCGGTCACCGAGACCAAGGCATCATCTTCAGAGTCAAAGAGAAAATTACCCGAGTGGTTTGCCAA AGGAAATGTGCCCTCAGCTGATACCGGCAGCTCATCATCAATGGCCAAGACCAAAAAGAAAGGTCTCTTT AGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211966 representing NM_011721
Red=Cloning site Green=Tags(s) METTSLQRKFPEWMSMQSQRCATEEKACVQKSVLEDNLPFLEFPGSIVYSYEASDCSFLSEDISMRLSDG DVVGFDMEWPPIYKPGKRSRVAVIQLCVSESKCYLFHISSMSVFPQGLKMLLENKSIKKAGVGIEGDQWK LLRDFDVKLESFVELTDVANEKLKCAETWSLNGLVKHVLGKQLLKDKSIRCSNWSNFPLTEDQKLYAATD AYAGLIIYQKLGNLGDTAQVFALNKAEENLPLEMKKQLNSISEEMRDLANRFPVTCRNLETLQRVPVILK SISENLCSLRKVICGPTNTETRLKPGSSFNLLSSEDSAAAGEKEKQIGKHSTFAKIKEEPWDPELDSLVK QEEVDVFRNQVKQEKGESENEIEDNLLREDMERTCVIPSISENELQDLEQQAKEEKYNDVSHQLSEHLSP NDDENDSSYIIESDEDLEMEMLKSLENLNSDVVEPTHSTWLEMGTNGRLPPEEEDGHGNEAIKEEQEEED HLLPEPNAKQINCLKTYFGHSSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYTGKIGIVIS PLISLMEDQVLQLELSNVPACLLGSAQSKNILGDVKLGKYRVIYITPEFCSGNLDLLQQLDSSIGITLIA VDEAHCISEWGHDFRSSFRMLGSLKTALPLVPVIALSATASSSIREDIISCLNLKDPQITCTGFDRPNLY LEVGRKTGNILQDLKPFLVRKASSAWEFEGPTIIYCPSRKMTEQVTAELGKLNLACRTYHAGMKISERKD VHHRFLRDEIQCVVATVAFGMGINKADIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHLLWAPADFNT SRNLLIEIHDEKFRLYKLKMMVKMEKYLHSSQCRRRIILSHFEDKCLQKASLDIMGTEKCCDNCRPRLNH CLTANNSEDASQDFGPQAFQLLSAVDILQEKFGIGIPILFLRGSNSQRLPDKYRGHRLFGAGKEQAESWW KTLSHHLIAEGFLVEVPKENKYIKTCSLTKKGRKWLGEASSQSPPSLLLQANEEMFPRKVLLPSSNPVSP ETTQHSSNQNPAGLTTKQSNLERTHSYKVPEKVSSGTNIPKKSAVMPSPGTSSSPLEPAISAQELDARTG LYARLVEARQKHANKMDVPPAILATNKVLLDMAKMRPTTVENMKQIDGVSEGKAALLAPLLEVIKHFCQV TSVQTDLLSSAKPHKEQEKSQEMEKKDCSLPQSVAVTYTLFQEKKMPLHSIAENRLLPLTAAGMHLAQAV KAGYPLDMERAGLTPETWKIIMDVIRNPPINSDMYKVKLIRMLVPENLDTYLIHMAIEILQSGSDSRTQP PCDSSRKRRFPSSAESCESCKESKEAVTETKASSSESKRKLPEWFAKGNVPSADTGSSSSMAKTKKKGLF S TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011721 |
| ORF Size | 6380 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011721.4, NP_035851.3 |
| RefSeq Size | 6476 bp |
| RefSeq ORF | 4206 bp |
| Locus ID | 22427 |
| UniProt ID | O09053 |
| Gene Summary | Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224398 | Wrn (untagged) - Mouse Werner syndrome homolog (human) (Wrn), transcript variant 1, (10ug) |
CNY 18,050.00 |
|
| MG226496 | Wrn (tGFP-tagged) - Mouse Werner syndrome homolog (human) (Wrn) transcript variant 1, (10ug) |
CNY 15,390.00 |
|
| MR226496 | Wrn (Myc-DDK-tagged) - Mouse Werner syndrome homolog (human) (Wrn), transcript variant 1 |
CNY 14,376.00 |
|
| MR226496L1 | Lenti ORF clone of Wrn (Myc-DDK-tagged) - Mouse Werner syndrome homolog (human) (Wrn), transcript variant 1 |
CNY 16,776.00 |
|
| MR226496L2 | Lenti ORF clone of Wrn (mGFP-tagged) - Mouse Werner syndrome homolog (human) (Wrn), transcript variant 1 |
CNY 15,870.00 |
|
| MR226496L3 | Lenti ORF clone of Wrn (Myc-DDK-tagged) - Mouse Werner syndrome homolog (human) (Wrn), transcript variant 1 |
CNY 15,870.00 |
|
| MR226496L4 | Lenti ORF clone of Wrn (mGFP-tagged) - Mouse Werner syndrome homolog (human) (Wrn), transcript variant 1 |
CNY 15,870.00 |
